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Plasmid#50920PurposeU6 driven sgRNA cloning vector where guide sequences are inserted between BfuAI sitesDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceJan. 22, 2014AvailabilityAcademic Institutions and Nonprofits only
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pEGFPC3-C2cd3
Plasmid#128871PurposeFor mammalian expression of mouse C2cd3DepositorInsertC2cd3 (C2cd3 Mouse)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHiUGE HA-epitope donor ORF1
Plasmid#200372PurposeTagging endogenously expressed proteins with HA epitope, C-terminalDepositorInsertHA epitope
UseTagsExpressionMammalianMutationNAPromoterAvailable sinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-ADP-MCS-FLAG
Plasmid#192360PurposeAdipocyte-specific overexpression of genesDepositorTypeEmpty backboneUseAAVTags3xflagExpressionMammalianMutationPromoteradiponectin promoterAvailable sinceNov. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-DU422gp160
Plasmid#100926PurposeMammalian expression plasmid for Env from the DU422.1 HIV-1 isolateDepositorInsertHIV-1 (DU422.1) Env
UseTagsCD5 leader sequenceExpressionMammalianMutationCodon-optimized synthetic genePromoterCMVAvailable sinceMarch 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-FLAG-CaMKIID
Plasmid#186827PurposeExpresses FLAG-CaMKIID in mammalian cellsDepositorInsertCamk2d (Camk2d Mouse)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceApril 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR_PGK_antiHer24D5-3_synNotch_Gal4VP64
Plasmid#85422PurposeLentiviral vector for expression of anti-Her2 scFv 24D5-3 Gal4VP64 synthetic NotchDepositorInsertanti-Her_4D5-3 synNotch
UseLentiviralTagsExpressionMutationPromoterPGKAvailable sinceNov. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-B41gp160
Plasmid#123247PurposeMammalian expression plasmid for Env from the B41 HIV-1 isolateDepositorInsertHIV-1 (B41) Env
UseTagsCD5 leader peptideExpressionMammalianMutationCodon-optimized synthetic genePromoterCMVAvailable sinceApril 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCS4333 yeTadA1.0-T7 RNAP
Plasmid#137735PurposepCS4333 CEN/ARS vector, PGAL1-yeTadA1.0-100AA linker-T7 RNAP-TCYC1, TRP1 selectable markerDepositorInsertyeTadA1.0-T7 RNAP
UseTagsExpressionBacterial and YeastMutationE366G (See depositor comment below)PromoterAvailable sinceJan. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHAGE TRE dCas9
Plasmid#50915PurposeTet-regulatable dCas9 lentiviral expression vectorDepositorInsertdCas9
UseCRISPR and LentiviralTagsExpressionMammalianMutationD10A, H840APromoterTREAvailable sinceFeb. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUB_smFLAG_KDM5B_MS2
Plasmid#81084PurposeTranslation reporter: Encodes KDM5B (1544 aa) fused to smFLAG (Spaghetti Monster FLAG: 10 copies of an epitope tag FLAG).DepositorInsertsmFLAG_KDM5B_24xMS2
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pI3-MTU-DO-G418
Plasmid#160023PurposeIntegrative vector for K. marxianus, targeting integration site I3 described in (Rajkumar et al.,2019). G418 selection.DepositorTypeEmpty backboneUseKluyveromyces marxianusTagsExpressionYeastMutationPromotern/aAvailable sinceNov. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-HaloTag N58-(GGS)X6-Lifeact
Plasmid#171436PurposeExpression of HaloTag N58-(GGS)X6-Lifeact in mammalian cellsDepositorInsertHaloTag N58-(GGS)X6-Lifeact
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
Halo-TNFa-RUSH
Plasmid#166901PurposeRUSH cargo: Str-KDEL_IRES_Tumor Necrosis Factor alpha (TNFa)-SBP-HaloDepositorInsertHaloTag
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLVE-eGFP
Plasmid#52581PurposeLentiviral transfer vector driving expression of eGFP in mammalian cellsDepositorInserteGFP
UseLentiviralTagsExpressionMammalianMutationPromoterEF-1alphaAvailable sinceJan. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PE2-SpG
Plasmid#159978PurposePrime editing in mammalian cellsDepositorInsertPE2-SpG
UseTagsBpSV40 NLSExpressionMammalianMutationPromoterCMVAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBMN(CMV-copGFP-Puro-SMAR)
Plasmid#80391Purposeexpression copGFP and Puromycine, with an S/MAR element for episomal maintainceDepositorInsertcopGFP-T2A-Puromycine
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHBintT
Plasmid#48134PurposeShuttle vector E. coli/B.meg., carrying a temperature sensitive origin of replication for Bacillus megaterium, selection marker tetracycline for B. megaterium, integration vectorDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEx-4T-1-YTHDF2
Plasmid#52301PurposeEncode YTHDF2 isoform 2 with N-terminal GST tag for E. coli expressionDepositorInsertYTHDF2 (YTHDF2 Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceApril 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHR_PGK_antiHer24D5-5_synNotch_Gal4VP64
Plasmid#85423PurposeLentiviral vector for expression of anti-Her2 scFv 24D5-5 Gal4VP64 synthetic NotchDepositorInsertanti-Her2_4D5-5 synNotch
UseLentiviralTagsExpressionMutationPromoterPGKAvailable sinceFeb. 14, 2018AvailabilityAcademic Institutions and Nonprofits only