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Plasmid#55631PurposeCre-Dependent Chloride ChannelDepositorInsertiC1C2-TS-EYFP
UseAAV and Cre/LoxTagsEYFPExpressionMammalianMutationC128APromoterEf1aAvailable sinceAug. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDB070.iodo.5
Plasmid#99397PurposeExpression plasmid encoding the evolved MjTyrRS variant Iodo.5RS for amber suppression with p-iodophenylalanineDepositorInsertPN25 TetR; Ptet Iodo.5RS; PproK tyrT(Opt,CUA)
UseTagsExpressionBacterialMutationPromoterPN25, Ptet, and PproKAvailable sinceNov. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yoEGFP-SpHis5
Plasmid#44836PurposeYeast tagging vector to create gene fusion with yeast optimized EGFP with SpHis5 selectionDepositorInsertyoEGFP
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
pS3
Plasmid#50596PurposeBackbone: pBbB5c Origin Of Replication:pBBR1 Selection Markers: Chloramphenicol Promoters: PLacUV5DepositorInsertsaroE
aroD
aroB
aroG*
ppsA
tktA
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterlacUV5Available sinceOct. 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNO183
Plasmid#101068PurposeppsR2-bphP1 bicistronic operon plasmidDepositorInsertsUseSynthetic BiologyTagsExpressionBacterialMutationinsert guanine at position 430 to fix frameshift …PromoterAvailable sinceNov. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTK297
Plasmid#59566PurposeSIN SP6 P1234 (nsP2 P726S) SGP(14) Kozak mKate-G8-L7Ae 4xFF4DepositorInsertSGP(14) Kozak mKate-G8-L7Ae 4xFF4
UseTagsExpressionMutationPromoterAvailable sinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
2xNLS-pMJ915v2
Plasmid#88916PurposeFor expression of modified SpyCas9 in e.coli.DepositorInsertsCas9
T7
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
1xNLS-pMJ915v2
Plasmid#88915PurposeFor expression of modified SpyCas9 in e.coli.DepositorInsertCas9
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCBL101.1
Plasmid#199724PurposeA pVS1-based T-DNA binary vector with NPTII as a plant selectable marker gene. BsaI site was removed.DepositorInsertMultiple Cloning Site, NPTII
UseTagsExpressionPlantMutation"GAGACC" to "GATACC"PromoterAvailable sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
p3E-mScarlet
Plasmid#140875PurposeMultisite Gateway middle entry vector for fusing mScarlet to the 3' end of an ORF.DepositorInsertmScarlet
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceFeb. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
4xNLS-pMJ915v2-sfGFP
Plasmid#88921PurposeFor expression of modified SpyCas9 in e.coli.DepositorInsertsCas9
T7
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceMay 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTiger-OptoSOS
Plasmid#86439PurposeSingle-construct blue light optogenetic Ras activator for use in DrosophilaDepositorInsertOptoSOS
UseTagstagRFPExpressionInsectMutationPromoterGal4-UASAvailable sinceFeb. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTR47m4
Plasmid#104068PurposeLuciferase Reporter for Formaldehyde SensingDepositorInsertsxluxAB
frmR
frm Promoter m4
UseLuciferase and Synthetic BiologyTagsExpressionBacterialMutation23r and 25th nucleotides in sequence mutated from…PromoterAvailable sinceMarch 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
psiCheck2-SV40p-3xFLAG-HSVTKp-mCherry
Plasmid#182377PurposeDual expression construct encoding 3xFLAG and mCherry from separate promotersDepositorInserts3xFlag
mCherry
UseTagsExpressionMammalianMutationPromoterHSV TK promoter and SV40 promoterAvailable sinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJAT77
Plasmid#204295PurposepJAT plasmid with multiple cloning site for introducing custom payload. No U6 promoter. Restriction sites present to introduce flanking U6 promoters.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
SuperNova-N1
Plasmid#131853PurposeExpresses SuperNova with C-terminal Myc-tag and N-terminal multiple cloning siteDepositorInsertSuperNova
UseTagsMyc tagExpressionMammalianMutationPromoterCMVAvailable sinceOct. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEN_Tmcs
Plasmid#25751PurposeEntry vector with TRE promoter followed by multiple cloning site.DepositorTypeEmpty backboneUseEntry vectorTagsExpressionMutationPromoterAvailable sinceJuly 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
pLSLGFP.R
Plasmid#51501PurposeSingle component LSL vector with GFP. Rep is expressed from the complementary sense LIR promoter. GFP is between LIR and SIR and is driven from a 2X35S promoter. Rep is within the repliconDepositorInsertGFP
UsePlant t-dna plasmidTagsExpressionMutationPromoter2x35SAvailable sinceMay 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBad-Xis/Int Reset Flipper
Plasmid#38207DepositorInsertsBxb1-Excisionase
Bxb1 Integrase
UseSynthetic Biology; Biobrick cloning vectorTagsExpressionMutationPromoterpBADAvailable sinceJuly 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAM5411
Plasmid#132663PurposeRSF1010-based broad host-range plasmid with RK2 bom site and pUC origin expressing YFP and Sp/Sm resistanceDepositorInsertsaadA
YFP
UseTagsExpressionBacterialMutationPromoterPaadA and PconIIAvailable sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only