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Plasmid#117728PurposeIntegration vector at an attB site in Bacteroides; contains 1kb cassette of aTC-regulatable promoter (RBS GH023) upstream of the NanoLuc geneDepositorInsertNanoLuc
UseLuciferaseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYPQ153
Plasmid#69304PurposeGateway entry vector with pco-dCas9-3X(SRDX)DepositorInsertpco-dCas9-3X(SRDX) (Plant codon-optimized)
UseCRISPRTags2XFLAGExpressionMutationSRDX (X3) repressor fusion; NLS fusionPromoterAvailable sinceOct. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDual-eGFP
Plasmid#63215PurposeExpression of eGFP in bacteria and in mammalian cells. Used as a reporter for quantifying gene targeting and recombineering in mammalian cells.DepositorInsertHis-T7-eGFP
UseLentiviralTagsHis6 and T7ExpressionBacterial and MammalianMutationPromoterCMV-EF1α hybrid (CEF)Available sinceApril 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCE059-SiT-Cas12a-[Activ]
Plasmid#128136PurposeExpress both AsCas12a fused to [Activ] activator domain and CRISPR arrays on a single transcriptDepositorInsertAsCas12a-[Activ]-Triplex
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceOct. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFH2.30_Gag-MCP-dpol-T2A-eGFP
Plasmid#205538PurposeExpress HIV Gag-MCP-dpol-T2A-eGFPDepositorInsertHIV Gag-MCP-dpol
UseTagsExpressionMammalianMutationpol frameshift sequence recodedPromoterCMVAvailable sinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBT137-splitC
Plasmid#122605Purposeclone M13 phage by SapI Golden GateDepositorInsertM13 bacteriophage genes
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCI118-SiT-Cas12a-[Ind]
Plasmid#128406PurposeInducible expression of both AsCas12a and CRISPR arrays on single transcriptsDepositorInsertAsCas12a-Triplex
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceOct. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDF0387 pcmv-hudiscas7-11-r978-gggs-r1294
Plasmid#186984Purposemammalian expression of cas7-11-r978-gggs-r1294DepositorInsertcas7-11-r978-gggs-r1294
UseTagsExpressionMammalianMutationr978-gggs-r1294 deletionPromoterAvailable sinceAug. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBT100.106b
Plasmid#122601Purposeclone APOBEC1 phage by SapI Golden GateDepositorInsertAPOBEC1
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-miniGFP1-P2A-FusionRed
Plasmid#188732PurposeExpress miniGFP1 and FusionRed in mammalian cellsDepositorInsertminiGFP1
UseAAVTagsFusionRedExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV hUbC-dCas9 VP64-T2A-GFP
Plasmid#53192PurposeCo-expresses human optimized S. pyogenes dCas9-VP64 and GFPDepositorInserthumanized dead Cas9 VP64 T2A GFP
UseCRISPR and LentiviralTagsFlagExpressionMammalianMutationD10A and H840APromoterhUbCAvailable sinceJune 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
Sar1b-YFP
Plasmid#166897Purposemammalian expression of Sar1b tagged with YFPDepositorInsertSar1b
UseTagsYFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG_smFP FLAG
Plasmid#59756Purposeencodes multiple copies of an epitope tag FLAGDepositorInsertsmFP _FLAG
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceOct. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHR_Gal4UAS_ Pembrolizumab_heavychain_T2A_ Pembrolizumab_lightchain_P2A_antiCTLA4scFv_PGK_mCherry
Plasmid#85436PurposeLentiviral vector for Gal4 inducible Pembrolizumab/anti-CTLA4 scFv and constitutive mCherry expressionDepositorInsertPembrolizumab and anti-CTLA4 scFv
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
Synapsin-cyto-mRuby3-iATPSnFR1.0
Plasmid#102557Purposeexpresses cytoplasmic ATP sensor in neurons with red reference proteinDepositorInsertmRuby3-iATPSnFR1.0
UseAAVTagsRuby3ExpressionMutationPromoterAvailable sinceJan. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
Cas9 sgRNA vector
Plasmid#68463PurposeCas9 sgRNA vector for cloningDepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceNov. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pVEGFR1_TEV(C)_TCS(Q'G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1
Plasmid#101103PurposeEncodes dCas9(C)-VP64 with SAM components fused to VEGFR1DepositorInsertVEGFR1-dCas9(C)
UseCRISPR and Synthetic BiologyTagsHAExpressionMammalianMutationPromoterAvailable sinceNov. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
(302) pcDNA3.1(+)-HA-LaG6-(EAAAK)4-OGT(4.5)
Plasmid#168192PurposeHA-tagged GFP nanobody Lag6 (310 nM affinity) fused to a truncated OGT of 4 TPRsDepositorInsertHA-LaG6-(EAAAK)4-OGT(4)
UseTagsHA-TagExpressionMammalianMutationPromoterT7/CMVAvailable sinceMay 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/cyto-iAspSnFR
Plasmid#201394PurposeCMV overexpression of cyto-iAspSnFR in mammalian cell linesDepositorInsertcyto-iAspSnFR
UseTagsExpressionMammalianMutationgltI5-253: Y42F, S72A, T92D, G111T, K192R, Y211W,…PromoterCMVAvailable sinceMay 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR_PGK_antiHer24D5-8_synNotch_Gal4VP64
Plasmid#85425PurposeLentiviral vector for expression of anti-Her2 scFv 24D5-8 Gal4VP64 synthetic NotchDepositorInsertanti-Her2_4D5-8_synNotch
UseLentiviralTagsExpressionMutationPromoterPGKAvailable sinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only