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Plasmid#194013PurposeThis plasmid is designed for use in a procedure to synthesize single-stranded DNA containing only the insert.DepositorInsertC16 Ex3 XTR f1
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pIS003
Plasmid#194014PurposeThis plasmid is designed for use in a procedure to synthesize single-stranded DNA containing only the insert.DepositorInsertC16 Ex4 XTR f1
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUCIDT-Washington
Plasmid#132758PurposeEncodes an iconic representation of a portrait of George WashingtonDepositorInsertWashington1
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUCIDT-Lincoln
Plasmid#132757PurposeEncodes an iconic representation of a portrait of Abraham LincolnDepositorInsertLincoln1
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEX-SaCas9-U6-sgKcna2
Plasmid#192796PurposeEncodes SaCas9 and an sgRNA targeting mouse Kcna2DepositorInsertsgKcna2
UseAAV and CRISPRTagsExpressionMutationPromoterU6Available sinceDec. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEX-SaCas9-U6-sgKcna6
Plasmid#192797PurposeEncodes SaCas9 and an sgRNA targeting mouse Kcna6DepositorInsertsgKcna6
UseAAV and CRISPRTagsExpressionMutationPromoterU6Available sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSiMPls_N + pSiMPls_C
Plasmid#167203PurposeSiMPl plasmid pair (pSiMPls) for use with spectinomycin/streptomycinDepositorInsertsEGFP
SmR 1-75 + gp41-1 N-intein
mRuby3
gp41-1 C-intein + SmR 76-263
UseTagsExpressionBacterialMutationPromoterAmpR, CAT, pBAD, and pTrcAvailable sinceOct. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGWT35S-sfGFP-SKL-Target2 (Mpphot)
Plasmid#186722PurposeTransient expression vector for SKLPT imaging for target 2 (Mpphot) in plant cells (Negative control vector).DepositorInsertsfGFP-SKL-Mphot
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMpGE010-Target2 (Mpphot)
Plasmid#186729PurposeBinary vector for CRISPR/Cas9 (target 2: Mpphot [negative control]) in plants (for Agrobacterium-mediated genetic transformation).DepositorInsertMphot
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGWT35S-sfGFP-SKL-Target1 (Mpphot)
Plasmid#186721PurposeTransient expression vector for SKLPT imaging for target 1 (Mpphot) in plant cells (Positive control vector).DepositorInsertsfGFP-SKL-Mphot
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMpGE_En03-sgRNA_Target2 (Mpphot)
Plasmid#186727PurposeGateway entry vector for sgRNA (target 2: Mpphot [negative control]). Transient expression of sgRNA (target 2: Mpphot) in plant cells.DepositorInsertsgRNA_Mphot
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMpGE_En03-sgRNA_Target1 (Mpphot)
Plasmid#186726PurposeGateway entry vector for sgRNA (target 1: Mpphot [positive control]). Transient expression of sgRNA (target 1: Mpphot) in plant cells.DepositorInsertsgRNA_Mphot
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR/Zeo-sfGFP-SKL
Plasmid#186720PurposeGateway entry vector encoding sfGFP with the two peroxisome-targeting signals (out of frame).DepositorInsertsfGFP-SKL
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR2_wt
Plasmid#149386PurposeP. aeruginosa PA14 CRISPR2 locusDepositorInsertPseudomonas aeruginosa PA14 CRISPR2 locus
UseTagsExpressionBacterialMutationPromoterpBADAvailable sinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
H2B-HT-emiRFP703
Plasmid#183985PurposeExpresses fluorescent HaloTag7 fusion protein in mammalian cells with nuclear localization.DepositorInsertH2B (H2BC11 Human)
UseTagsHaloTag, emiRFP703ExpressionMammalianMutationPromoterAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
H2B-HT-mGold
Plasmid#183988PurposeExpresses fluorescent HaloTag7 fusion protein in mammalian cells with nuclear localization.DepositorInsertH2B (H2BC11 Human)
UseTagsHaloTag, mGoldExpressionMammalianMutationPromoterAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCS2+-wbp2nlT45A
Plasmid#172464Purposeexpresses protein with one aa substitution to affect potential phosphorylationDepositorInsertwbp2nlT45A
UseXenopus expressionTagsnoneExpressionMutationchanged Threonine at aa45 to AlaninePromoterSP6Available sinceSept. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA_GFP_hTLN2-FL-LBD
Plasmid#164841PurposeExpression of GFP tagged FL human talin2 mutantDepositorInsertTLN2 (TLN2 Human)
UseTagsHis10-GFPExpressionMammalianMutationLipid binding deficient mutationsPromoterAvailable sinceApril 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28_hTLN2-head-LBD
Plasmid#164837PurposeExpression of human talin2 head mutantDepositorInsertTLN2 (TLN2 Human)
UseTagsHis8ExpressionBacterialMutationLipid binding deficient mutationsPromoterAvailable sinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSKB3SaGGR I206F (JBEI-3678)
Plasmid#110149PurposeSaGGR site-directed mutation of I206FDepositorInsertGGR
UseTagsExpressionBacterialMutationSaGGR site-directed mutation of I206FPromoterT7Available sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only