Addgene

We narrowed to 15,057 results for: grna

Showing: 10661 - 10680 of 15057 results

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THI VP64 pALD4 T3

Plasmid

#161484

Purpose

CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pALD4_THI11_T3_gRNA_MS2

Depositor

Brigitte Gasser, Diethard Mattanovich

Article

Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

Inserts

dCAS9
MS2-VP64
gRNA (targeting T3 of pTHI11 promoter)

Use

Tags

Expression

Yeast

Mutation

Promoter

pALD4, pPOR1, and pTEF2

Available since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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THI VP64 pALD4 T1

Plasmid

#161482

Purpose

CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pALD4_THI11_T1_gRNA_MS2

Depositor

Brigitte Gasser, Diethard Mattanovich

Article

Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

Inserts

dCAS9
MS2-VP64
gRNA (targeting T1 of pTHI11 promoter)

Use

Tags

Expression

Yeast

Mutation

Promoter

pALD4, pPOR1, and pTEF2

Available since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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pBbdCas9H(-10TC)_arr2

Plasmid

#149587

Purpose

parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

PflaB, PpQE30

Available since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pLKO.1 Puro shRNA PIK3C2G KD1

Plasmid

#162008

Purpose

PIK3C2gamma shRNA

Depositor

David Bryant

Article

Roman-Fernandez et al Nat Commun. 2018 Nov 28;9(1):5041. doi:

Insert

PIK3C2G (PIK3C2G Canis Lupus)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pLKO.1 Puro shRNA SNX9 KD1

Plasmid

#162014

Purpose

SNX9 shRNA

Depositor

David Bryant

Article

Roman-Fernandez et al Nat Commun. 2018 Nov 28;9(1):5041. doi:

Insert

SNX9 (SNX9 Canis Lupus)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO.1 Puro shRNA SNX9 KD2

Plasmid

#162015

Purpose

SNX9 shRNA

Depositor

David Bryant

Article

Roman-Fernandez et al Nat Commun. 2018 Nov 28;9(1):5041. doi:

Insert

SNX9 (SNX9 Canis Lupus)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO.1 Puro shRNA PIK3C2A KD5

Plasmid

#162006

Purpose

PIK3C2alpha shRNA

Depositor

David Bryant

Article

Roman-Fernandez et al Nat Commun. 2018 Nov 28;9(1):5041. doi:

Insert

PIK3C2A (PIK3C2A Canis Lupus)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO.1 Puro shRNA PTEN KD4

Plasmid

#162010

Purpose

PTEN shRNA

Depositor

David Bryant

Article

Roman-Fernandez et al Nat Commun. 2018 Nov 28;9(1):5041. doi:

Insert

PTEN (PTEN Canis Lupus)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S(-10TC)_arr2

Plasmid

#149582

Purpose

parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

PflaB, PpQE30

Available since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pBbdCas9G(-10TC)_arr2

Plasmid

#149585

Purpose

parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

PflaB, PpQE30

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBbdCas9B(-10TC)_arr2

Plasmid

#149586

Purpose

parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

PflaB, PpQE30

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBbdCas9B_arr2

Plasmid

#149572

Purpose

parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

PflaB, PpQE30

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBbdCas9H_arr2

Plasmid

#149573

Purpose

parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

PflaB, PpQE30

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S(RBSmut)_arr2

Plasmid

#149575

Purpose

parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

PflaB, PpQE30

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBbdCas9B(RBSmut)_arr2

Plasmid

#149579

Purpose

parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

PflaB, PpQE30

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pBbdCas9H(RBSmut)_arr2

Plasmid

#149580

Purpose

parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

PflaB, PpQE30

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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sg2

Plasmid

#113967

Purpose

Single short guide RNA targeting TACCACATTTGTAGAGGTT

Depositor

Jordan Green

Article

Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6

Insert

sg2

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

May 28, 2020

Availability

Academic Institutions and Nonprofits only

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sg3

Plasmid

#113968

Purpose

Single short guide RNA targeting CAATGTATCTTATCATGTC

Depositor

Jordan Green

Article

Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6

Insert

sg3

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

May 28, 2020

Availability

Academic Institutions and Nonprofits only

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sg2+sg3

Plasmid

#113970

Purpose

Double short guide RNA targeting TACCACATTTGTAGAGGTT & CAATGTATCTTATCATGTC

Depositor

Jordan Green

Article

Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6

Insert

sg2+sg3

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

May 28, 2020

Availability

Academic Institutions and Nonprofits only

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pX300A-G+Nanog

Plasmid

#140280

Purpose

CRISPR/Cas9 plasmid encoding Cas9 and sgRNA against gBait and Nanog locus

Depositor

Yusuke Miyanari

Article

Kurihara et al Mol Cell. 2020 May 7;78(3):493-505.e8. d

Insert

Cas9

Use

Tags

Expression

Mammalian

Mutation

Promoter

CBH

Available since

May 4, 2020

Availability

Academic Institutions and Nonprofits only

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