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Plasmid#102829PurposeBacterial expression of Cysteine-modified SpyCatcher002 allowing reaction with thiol coupling reagentsDepositorInsertCys-SpyCatcher002
UseTagsGSSGS, His6, and TEV cleavage siteExpressionBacterialMutationCysteine residue in N-terminal regionPromoterT7Available sinceDec. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-DU422gp160
Plasmid#100926PurposeMammalian expression plasmid for Env from the DU422.1 HIV-1 isolateDepositorInsertHIV-1 (DU422.1) Env
UseTagsCD5 leader sequenceExpressionMammalianMutationCodon-optimized synthetic genePromoterCMVAvailable sinceMarch 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-mt-ro1GFP
Plasmid#82407PurposeExpression of redox sensitive GFP with a mitochondrial targeting sequence in mammalian cellsDepositorInsertro1GFP
UseTagsleader sequence of the E1α subunit of pyruvate de…ExpressionMammalianMutationC48S/F64L/T65S/S147C/Q204CPromoterCMVAvailable sinceSept. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
mApple-hCOL1A1
Plasmid#140113PurposeExpresses fluorescently-tagged human Type I procollagen α1 chain with mApple replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage siteDepositorInsertType I procollagen α1 (COL1A1 Human)
UseTagsmAppleExpressionMammalianMutationCOL1A1 exons 2-3 replaced by fluorescent tagPromoterCMVAvailable sinceMay 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMEH280 NLS dTomato 3x high affinity MS2 loops
Plasmid#168211PurposeMammalian expression of nuclear-localized dTomato with 3 high affinity MS2 loopsDepositorInsertNLS dTomato
UseLentiviralTagsNLSExpressionMammalianMutationPromoterCMVAvailable sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046809
Plasmid#145685PurposeBacterial Expression plasmid for SARS-CoV-2 2'-O-ribose methyltransferaseDepositorInsertSARS-CoV-2 2'-O-ribose methyltransferase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;6xHISExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
cr3Ec
Plasmid#184860Purposecr3 helper plasmid for recombination and Cas9 induction; harbors the Cas9 endonuclease and an E.coli-specific recombination systemDepositorInsertCas9, gam, bet, exo
UseTagsCas9 ssrA degradation tagExpressionBacterialMutationPromoterAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pX330-Flag-wtSpCas9-H840A
Plasmid#80453PurposeSpCas9 mammalian expression vectorDepositorInsertSpCas9_H840A
UseCRISPRTags3xFLAG and NLSExpressionMammalianMutationPromoterCBhAvailable sinceAug. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAG-nDsRedFL-intron
Plasmid#177804PurposeAn intron is inserted into the DsRed gene with a nuclear transfer signal and a 3xFlag tag. The intron can be digested with EcoRV and any pre-miRNA can be inserted.DepositorInsertintron
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-B41gp160
Plasmid#123247PurposeMammalian expression plasmid for Env from the B41 HIV-1 isolateDepositorInsertHIV-1 (B41) Env
UseTagsCD5 leader peptideExpressionMammalianMutationCodon-optimized synthetic genePromoterCMVAvailable sinceApril 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mCherry-LKB1(WT)
Plasmid#84639PurposemCherry-tagged, wild-type LKB1DepositorInsertmCherry-LBK1 (STK11 Human, Synthetic)
UseTags6xHis, T7 tag (gene 10 leader), Xpress (TM) tag, …ExpressionMammalianMutationPromoterCMVAvailable sinceMay 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
PEP110E
Plasmid#97396Purposebinary construct of nuclear mCherry with sfGFP11 tag for plant expressionDepositorInsertmCherry-sfGFP11
UseTagsNucleus targetExpressionPlantMutationPromoterAvailable sinceSept. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_IgKchL_HA_STf_myc_PDGFRtmb
Plasmid#182009PurposeCMV overexpression of SNAP-tag fast at the outer leaflet of the plasma membrane of mammalian cell linesDepositorInsertIgKchL_HA_STf_myc_PDGFRtmb
UseTagsExpressionMammalianMutationFast mutation on SNAP-tag E30RPromoterCMVAvailable sinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046760
Plasmid#145600PurposeBacterial Expression plasmid for SARS-CoV-2 helicaseDepositorInsertSARS-CoV-2 helicase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;6xHISExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pNES-iRFP-C1
Plasmid#116863Purposecytosolic iRFPDepositorInsertiRFP713
UseTagsX.leavis map2k1.L(32-44)ExpressionMammalianMutationPromoterAvailable sinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKL1-222
Plasmid#219409PurposeNuclear expression of Synrophin PDZ clamp proteinDepositorInsertpTEF1-NLS-(Syntrophin-PDZ)x4-tADH1
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLENTI_RPS10
Plasmid#127129DepositorInsertRPS10 (RPS10 Human)
UseLentiviralTagsExpressionMutationK24R- please see depositor commentsPromoterAvailable sinceAug. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046814
Plasmid#145601PurposeBacterial Expression plasmid for SARS-CoV-2 E (envelope)DepositorInsertSARS-CoV-2 E (envelope) (E Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;6xHISExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 28, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046979
Plasmid#145611PurposeBacterial Expression plasmid for SARS-CoV-2 nsp7DepositorInsertSARS-CoV-2 nsp7 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;6xHISExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 28, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
Nuc-ABKAR
Plasmid#61511PurposeExpression of nuclear AMPK biosensorDepositorInsertAMPK Biosensor
UseTagsNLS (see comments)ExpressionMammalianMutationPromoterCMVAvailable sinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only