Addgene

We narrowed to 14,149 results for: LEA

Showing: 10661 - 10680 of 14149 results

Results per page:

H2A-mTurquoise-RHOG-QL-deltaCAAX

Plasmid

#176096

Purpose

Constitutively active, nuclear localized RhoG

Depositor

Dorus Gadella

Article

Mahlandt et al J Cell Sci. 2023 May 15;136(10):jcs26080

Insert

RHOG (RHOG Human)

Use

Tags

Expression

Mammalian

Mutation

QL

Promoter

Availability

Academic Institutions and Nonprofits only

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pBH31(gfcD)

Plasmid

#176199

Purpose

Encodes pelC signal sequence followed by His6-GfcD. Should secrete to periplasm

Depositor

Mark Saper

Article

Larson et al PLoS One. 2021 Nov 15;16(11):e0259900. d

Insert

gfcD

Use

Tags

pelC leader-6xHis

Expression

Bacterial

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pBH31(gfcD-10xHis)

Plasmid

#176200

Purpose

Encodes pelC signal sequence followed by His10-GfcD. Should secrete to periplasm

Depositor

Mark Saper

Article

Larson et al PLoS One. 2021 Nov 15;16(11):e0259900. d

Insert

gfcD

Use

Tags

pelC leader-10xHis

Expression

Bacterial

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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hPCNA-S228I

Plasmid

#190938

Purpose

untagged human PCNA with a hypomorphic disease-associated serine to isoleucine mutation at residue 228

Depositor

Brian Kelch

Article

Duffy et al J Mol Biol. 2016 Mar 27;428(6):1023-1040

Insert

Proliferating Cell Nuclear Antigen (PCNA Human)

Use

Tags

Expression

Bacterial

Mutation

changed serine 228 to isoleucine

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pET-28a(+)-His(6)-AtPCO1(wt)

Plasmid

#191798

Purpose

Full length wt AtPCO1 with Nt-His tag

Depositor

Emily Flashman

Article

White et al J Biol Chem. 2018 Jul 27;293(30):11786-1

Insert

AtPCO1

Use

Tags

6xHis, Thrombin cleavage site

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pET-28a(+)-His(6)-AtPCO2(wt)

Plasmid

#191799

Purpose

Full length wt AtPCO2 with Nt-His tag

Depositor

Emily Flashman

Article

White et al J Biol Chem. 2018 Jul 27;293(30):11786-1

Insert

AtPCO2

Use

Tags

6xHis, Thrombin cleavage site

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pET-28a(+)-His(6)-AtPCO3(wt)

Plasmid

#191800

Purpose

Full length wt AtPCO3 with Nt-His tag

Depositor

Emily Flashman

Article

White et al J Biol Chem. 2018 Jul 27;293(30):11786-1

Insert

AtPCO3

Use

Tags

6xHis, Thrombin cleavage site

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pET-28a(+)-His(6)-TEV-AtPCO4-1(wt)

Plasmid

#191801

Purpose

Full length wt AtPCO4 with Nt-His tag

Depositor

Emily Flashman

Article

White et al J Biol Chem. 2018 Jul 27;293(30):11786-1

Insert

AtPCO4-1

Use

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pET-28a(+)-His(6)-TEV-AtPCO5(wt)

Plasmid

#191803

Purpose

Full length wt AtPCO5 with Nt-His tag

Depositor

Emily Flashman

Article

White et al J Biol Chem. 2018 Jul 27;293(30):11786-1

Insert

AtPCO5

Use

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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Clomeleon

Plasmid

#203723

Purpose

A genetically encoded ratiometric optical indicator for chloride ions, to detect changes in intracellular chloride concentration

Depositor

George Augustine

Article

Kuner et al Neuron. 2000 Sep;27(3):447-59. doi: 10.1

Insert

cyan fluorescent protein linked yellow florescent protein

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Cyan Fluorescent Protein, Topaz Fluorescent Prote…

Promoter

Availability

Academic Institutions and Nonprofits only

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PB-NES-ZapCV2-Zinc dead

Plasmid

#203850

Purpose

Expressing zinc dead sensor NES-ZapCV2-Ala in cytoplasm of mammalian cells

Depositor

Amy Palmer

Article

Rakshit et al Cell Rep. 2023 Jun 16;42(6):112656. doi:

Insert

ZapCV2-Ala zinc dead sensor

Use

Tags

Nuclear Export Signal

Expression

Mammalian

Mutation

Cys and His in Zap1 is mutated with Ala

Promoter

CMV promoter

Availability

Academic Institutions and Nonprofits only

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pDGE1109

Plasmid

#202773

Purpose

CRISPR-KO with TRE2 exonuclease co-expression in Arabidopsis thaliana

Depositor

Johannes Stuttmann

Article

Ordon et al Plant Methods. 2023 Mar 28;19(1):30. doi

Inserts

FAST marker
pRPS5a:TREX2-zCas9_ttriple
ccdB cassette (BsaI-excisable)

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pDGE1177

Plasmid

#202775

Purpose

CRISPR-KO with TRE2 exonuclease co-expression in Arabidopsis thaliana

Depositor

Johannes Stuttmann

Article

Ordon et al Plant Methods. 2023 Mar 28;19(1):30. doi

Inserts

FAST marker
pRPS5a:TREX2-zCas9_tNbEU
ccdB cassette (BsaI-excisable)

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_Lyn11-STf

Plasmid

#182008

Purpose

CMV overexpression of SNAP-tag fast at the inner leaflet of the plasma membrane of mammalian cell lines

Depositor

Kai Johnsson

Article

Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

Insert

Lyn11-STf

Use

Tags

Expression

Mammalian

Mutation

Fast mutation on SNAP-tag E30R

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_IgKchL_HA_STf_myc_PDGFRtmb

Plasmid

#182009

Purpose

CMV overexpression of SNAP-tag fast at the outer leaflet of the plasma membrane of mammalian cell lines

Depositor

Kai Johnsson

Article

Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

Insert

IgKchL_HA_STf_myc_PDGFRtmb

Use

Tags

Expression

Mammalian

Mutation

Fast mutation on SNAP-tag E30R

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Dronpa-Kras

Plasmid

#182329

Purpose

Plasma membrane-targeted Dronpa. Fluctuating green-fluorescent label for live-cell super-resolution imaging.

Depositor

Jin Zhang

Article

Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951

Insert

Dronpa-Kras

Use

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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FnRPR9-10

Plasmid

#182568

Purpose

Human fibronectin fragment, type-III repeats 9-10, R1374A, P1376A, R1379A mutant

Depositor

Timothy Springer

Article

Su et al J Biol Chem. 2022 Sep;298(9):102323. doi

Insert

Human fibronectin fragment, type-III repeats 9-10

Use

Tags

His and TEV cleavage site after His tag

Expression

Bacterial

Mutation

R1374A, P1376A, R1379A

Promoter

Availability

Academic Institutions and Nonprofits only

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FnRPR8-10

Plasmid

#182570

Purpose

Human fibronectin fragment, type-III repeats 8-10, R1374A, P1376A, R1379A mutant

Depositor

Timothy Springer

Article

Su et al J Biol Chem. 2022 Sep;298(9):102323. doi

Insert

Human fibronectin fragment, type-III repeats 8-10

Use

Tags

His and TEV cleavage site after His tag

Expression

Bacterial

Mutation

R1374A, P1376A, R1379A

Promoter

Availability

Academic Institutions and Nonprofits only

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p3xnls-mScarlet3_N1

Plasmid

#189775

Purpose

Construct for labelling cell nuclei in mammalian cells using nuclear targeted mScarlet3

Depositor

Dorus Gadella

Article

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Insert

3xnls-mScarlet3

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pmScarlet3-LaminB_C1

Plasmid

#189776

Purpose

Construct for labelling nuclear envelopes in mammaiian cells using mScarlet3-LaminB fusion

Depositor

Dorus Gadella

Article

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Insert

mScarlet3-LaminB

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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