Addgene

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pDR-AmTrac

Plasmid

#47770

Purpose

expresses AmTrac in yeast

Depositor

Wolf Frommer

Article

De Michele et al Elife. 2013 Jul 2;2:e00800.

Insert

AmTrac

Use

Tags

mcpGFP

Expression

Yeast

Mutation

Promoter

PMA

Available since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pWS939

Plasmid

#123051

Purpose

Pheromone responsive transcription factor

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

Ste12 [3]

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 8, 2019

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 YWHAB

Plasmid

#67063

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

YWHAB (YWHAB Human)

Use

Cell-free expression

Tags

EGFP and His

Expression

Mutation

Promoter

T7

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pCellFree_G03 TYK2

Plasmid

#67133

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

TYK2 (TYK2 Human)

Use

Cell-free expression

Tags

EGFP and His

Expression

Mutation

A435S

Promoter

T7

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
HS-H2B-RFP

Plasmid

#105953

Purpose

heat shock inducible transgenesis

Depositor

Caren Norden

Article

Strzyz et al Dev Cell. 2015 Jan 26;32(2):203-19. doi:

Insert

H2B

Use

Tags

RFP

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 19, 2018

Availability

Academic Institutions and Nonprofits only

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UBE2G2_pCSdest

Plasmid

#53938

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

UBE2G2 (UBE2G2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

SP6 and CMV

Available since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits
Nano-lantern(ATP1)/pENTR1A

Plasmid

#51993

Purpose

Gateway entry vector for Nano-lantern-based luminescent ATP indicator

Depositor

Takeharu Nagai

Article

Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

Insert

Nano-lantern_ATP1

Use

Entry vector

Tags

Expression

Mutation

S257G in Rluc8

Promoter

Available since

March 24, 2014

Availability

Academic Institutions and Nonprofits only
pWS067

Plasmid

#123044

Purpose

_-factor GPCR

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

Ste2 [3]

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pWS053

Plasmid

#123025

Purpose

Pheromone responsive promoter

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

pFIG1 [2]

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pWS060

Plasmid

#123024

Purpose

Pheromone responsive promoter

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

pFUS1 [2]

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 3, 2019

Availability

Academic Institutions and Nonprofits only

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pCM3.06

Plasmid

#19602

Depositor

Geraldine Seydoux

Article

Lee et al PLoS Genet. 2007 Dec 28. 3(12):e233.

Insert

fbf-1 ORF+3' in pDONR201 (fbf-1 Nematode)

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Nov. 10, 2008

Availability

Academic Institutions and Nonprofits only

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CH42: Para_lac_bD

Plasmid

#104868

Purpose

CH42: Para_lac_bD

Depositor

Matthew Bennett

Article

Chen et al Nat Commun. 2018 Jan 4;9(1):64. doi: 10.

Insert

Hybrid Promoter : Para_lac_bD

Use

Tags

Expression

Bacterial

Mutation

Promoter

Hybrid Promoter : Para_lac_bD

Available since

Jan. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pUAST td tomato Rab35 CA

Plasmid

#58383

Purpose

expression of drosophila tdtomato-Rab35 constitutively active mutant

Depositor

Matthew Scott

Article

Zhang et al Science. 2009 Sep 4;325(5945):1250-4. do

Insert

Rab35 (Rab35 Fly)

Use

Tags

td tomato

Expression

Insect

Mutation

Q67L (constitutive active)

Promoter

GAL4

Available since

July 21, 2014

Availability

Academic Institutions and Nonprofits only
pDR-AmTrac1;2

Plasmid

#47765

Purpose

expresses AmTrac1;2 in yeast

Depositor

Wolf Frommer

Article

De Michele et al Elife. 2013 Jul 2;2:e00800.

Insert

AmTrac1;2

Use

Tags

mcpGFP

Expression

Yeast

Mutation

Promoter

PMA

Available since

Sept. 16, 2013

Availability

Academic Institutions and Nonprofits only
pDR-AmTrac-LS

Plasmid

#47767

Purpose

expresses AmTrac-LS in yeast

Depositor

Wolf Frommer

Article

De Michele et al Elife. 2013 Jul 2;2:e00800.

Insert

AmTrac-LS

Use

Tags

mcpGFP

Expression

Yeast

Mutation

Promoter

PMA

Available since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only
TPTE_pCSdest

Plasmid

#53788

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

TPTE (TPTE Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

SP6 and CMV

Available since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits
pLSLZ

Plasmid

#51495

Purpose

pLSL with Zif268:FokI coding sequence followed by approximately 3kb 'filler' sequence, Rep is not included on this plasmid

Depositor

Daniel Voytas

Article

Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:

Inserts

Zif268:FokI
3kb filler sequence

Use

Plant t-dna plasmid

Tags

SV40 NLS

Expression

Mutation

Promoter

virion-sense LIR and 2x35S

Available since

May 1, 2014

Availability

Academic Institutions and Nonprofits only

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NHA-Delta 11

Plasmid

#36926

Depositor

Edward Chan

Article

Yao et al Nucleic Acids Res. 2011 Apr;39(7):2534-4

Insert

TNRC6A (TNRC6A Human)

Use

Tags

NHA

Expression

Mammalian

Mutation

aa 722-941 only

Promoter

Available since

May 24, 2012

Availability

Academic Institutions and Nonprofits only
pDEST47-SARA

Plasmid

#67473

Purpose

Mammalian expression of hSara with stop codon (native expression)

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

SARA (SAR1B Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
TU#1448_unc-73b_cDNA

Plasmid

#82362

Purpose

express unc-73b cDNA

Depositor

Martin Chalfie

Article

Zheng et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

Insert

unc-73 b isoform (unc-73 Nematode)

Use

Tags

Expression

Worm

Mutation

Promoter

none

Available since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

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