Showing: 10621 - 10640 of 20620 results
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Plasmid#102440PurposeFor PCR amplification to create paired gRNAs to clone into Golden Gate gRNA expression vectors (e.g. pSB700 Plasmid #64046)DepositorInsert7SK paired gRNA insert
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pSLQ1870-1 pHR: U6-SpsgTRE3G CMV-mCherry
Plasmid#84248PurposeExpresses Sp sgTRE3G gRNA with a mCherry fluorescent markerDepositorInsertSp sgTRE3G
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromotermouse U6AvailabilityAcademic Institutions and Nonprofits only -
pSLQ1869-1 pHR: U6-SpsgSV40 CMV-mCherry
Plasmid#84249PurposeExpresses Sp sgSV40 gRNA with a mCherry fluorescent markerDepositorInsertSp sgSV40
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromotermouse U6AvailabilityAcademic Institutions and Nonprofits only -
pSLQ2806-2 pHR: U6-Sasgv2TRE3G CMV-mCherry
Plasmid#84250PurposeExpresses optimized Sa sgTRE3G gRNA with a mCherry fluorescent markerDepositorInsertSa sgTRE3G
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromotermouse U6AvailabilityAcademic Institutions and Nonprofits only -
pTMt_TCS(Q’G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1
Plasmid#101102PurposeEncodes dCas9(C)-VP64 with SAM components fused to transmembrane tetherDepositorInsertTMt-dCas9(C)-VP64
UseCRISPR and Synthetic BiologyTagsHA and MycExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pBDKRB2_TCS(Q’G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1
Plasmid#101107PurposeEncodes dCas9(C)-VP64 with SAM components fused to BDKRB2DepositorInsertBDKRB2-dCas9(N)
UseCRISPR and Synthetic BiologyTagsFlag and HAExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pX330-NEAT1pr_v1
Plasmid#97081PurposeEncodes an sgRNA that creates a DSB at the promoter of human NEAT1 geneDepositorInsertsgRNA NEAT1pr_v1
UseCRISPRTagsExpressionMutationPromoterU6AvailabilityAcademic Institutions and Nonprofits only -
pX330-NEAT1pr_v2
Plasmid#97082PurposeEncodes an sgRNA that creates a DSB at the promoter of human NEAT1 geneDepositorInsertsgRNA NEAT1pr_v2
UseCRISPRTagsExpressionMutationPromoterU6AvailabilityAcademic Institutions and Nonprofits only -
pX330-NEAT1m_v1
Plasmid#97083PurposeEncodes an sgRNA that creates a DSB immediately downstream the poly(A) signal of human NEAT1_1 isoformDepositorInsertsgRNA NEAT1m_v1
UseCRISPRTagsExpressionMutationPromoterU6AvailabilityAcademic Institutions and Nonprofits only -
pX330-GFP-SFPQ
Plasmid#97084PurposeEncodes an sgRNA that creates a DSB at the promoter of human SFPQ gene.DepositorInsertsgRNA GFP-SFPQ
UseCRISPRTagsExpressionMutationPromoterU6AvailabilityAcademic Institutions and Nonprofits only -
pX335-NEAT1_IS_v1
Plasmid#97085PurposeEncodes an sgRNA that creates a SSB near the poly(A) site of human NEAT1_1 isoform.DepositorInsertsgRNA NEAT1_IS_v1
UseCRISPRTagsExpressionMutationPromoterU6AvailabilityAcademic Institutions and Nonprofits only -
pX335-NEAT1_IS_v2
Plasmid#97086PurposeEncodes an sgRNA that creates a SSB near the poly(A) site of human NEAT1_1 isoform.DepositorInsertsgRNA NEAT1_IS_v2
UseCRISPRTagsExpressionMutationPromoterU6AvailabilityAcademic Institutions and Nonprofits only -
pXPR_124
Plasmid#133461PurposeEF1a promoter expresses SpyoCas9 and P2A provides EGFPDepositorInsertSpyoCas9
UseCRISPR and LentiviralTagsEGFPExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pCFD5-frame_selector_0,1,2
Plasmid#131152PurposeDrosophila expression of frame selector sgRNAs 0,1, and 2 that target the CRISPaint site for homology-independent knock-inDepositorInsertCRISPaint frame selector sgRNAs 0,1,2
UseCRISPRTagsExpressionInsectMutationPromoterdU6:3AvailabilityAcademic Institutions and Nonprofits only -
pCAG-T3-HypaCAS9-pA
Plasmid#131467PurposeExpresses HypaCas9 nuclease in mammalian cells. Used to modify genome of mouse embroysDepositorInsertCodon optimized HypaCas9
UseCRISPRTagsFlag, NLS, and polyAExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
FTO-dCas9
Plasmid#134781PurposeExpression fusion protein FTO-dCas9 in Mammalian cellsDepositorInsertFTO and inactive dCas9
UseTagsExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
ALKBH5-dCas9
Plasmid#134783PurposeExpression fusion protein ALKBH5-dCas9 in Mammalian cellsDepositorInsertALKBH5 and inactive dCas9
UseTagsExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
ALKBH5H204A-dCas9
Plasmid#134784PurposeExpression fusion protein ALKBH5H204A-dCas9 in Mammalian cellsDepositorInsertALKBH5 and inactive dCas9
UseTagsExpressionMammalianMutationH204APromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
N-4-BE
Plasmid#135019PurposeC-T base editing using LjCDA1L1_4 fused to N terminal of nCas9DepositorInsertLjCDA1L1_4
UseCRISPRTagsExpressionMammalianMutationPromoterU6 with EF-1alpha intronAvailabilityAcademic Institutions and Nonprofits only -
N-8-BE
Plasmid#135023PurposeC-T base editing using LpCDA1L1_1 fused to N terminal of nCas9DepositorInsertLpCDA1L1_1
UseCRISPRTagsExpressionMammalianMutationPromoterU6 with EF-1alpha intronAvailabilityAcademic Institutions and Nonprofits only
Showing: 10621 - 10640 of 20620 results