Addgene

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pCx55.5-TrpR

Plasmid

#86373

Purpose

for zebrafish transgenesis, expression of TrpR from Cx55.5 promoter, also expresses mCherry

Depositor

Rachel Wong

Article

Yoshimatsu et al Nat Commun. 2016 Feb 3;7:10590. doi: 10.

Insert

TrpR

Use

Zebrafish

Tags

Expression

Mutation

Promoter

cx55.5

Available since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pWS932

Plasmid

#123055

Purpose

Pheromone responsive domain

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

PRD [3b]

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pWS2119

Plasmid

#123048

Purpose

PbPeptide GPCR

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

PbSte2 [3]

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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SH3BGR_pCSdest

Plasmid

#53932

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

SH3BGR (SH3BGR Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

SP6 and CMV

Available since

Sept. 8, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pE1B-C-F/Sox10-MCS9

Plasmid

#20248

Depositor

William Pavan

Article

Antonellis et al PLoS Genet. 2008 . 4(9):e1000174.

Insert

Sox10-MCS9

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2009

Availability

Academic Institutions and Nonprofits only

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PCBP3_pCSdest

Plasmid

#53957

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

PCBP3 (PCBP3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

SP6 and CMV

Available since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits
pYPQ159

Plasmid

#69308

Purpose

Gateway entry vector with hSpCas9D10A

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Insert

hSpCas9D10A (Human codon optimized)

Use

CRISPR

Tags

3XFLAG

Expression

Mutation

D10A mutation; NLS fusion

Promoter

Available since

Nov. 4, 2015

Availability

Academic Institutions and Nonprofits only

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pAW-GFP-2xmiR-34 (pAW-AS-25)

Plasmid

#40833

Purpose

miRNA sensor used in Drosophila cell lines

Depositor

Phillip Zamore

Article

Ameres et al Science. 2010 Jun 18;328(5985):1534-9.

Insert

Two target sites perfectly complementary to miR-34

Use

Mirna reporter

Tags

EGFP

Expression

Insect

Mutation

Promoter

Actin5C

Available since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only
SUN2delSUN

Plasmid

#130835

Purpose

Expression of plant LINC fluorescent fusion protein for cell biology

Depositor

Hank Bass

Article

Gumber et al J Cell Sci. 2019 Feb 11;132(3). pii: jcs

Insert

SUN2

Use

Tags

mCherry-FLAG-HA

Expression

Plant

Mutation

Deletion of SUN domain

Promoter

CaMV 35S

Available since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pE1B-C-F/Sox10-MCS2

Plasmid

#20241

Depositor

William Pavan

Article

Antonellis et al PLoS Genet. 2008 . 4(9):e1000174.

Insert

Sox10-MCS2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2009

Availability

Academic Institutions and Nonprofits only

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pE1B-C-F/Sox10-MCS6

Plasmid

#20245

Depositor

William Pavan

Article

Antonellis et al PLoS Genet. 2008 . 4(9):e1000174.

Insert

Sox10-MCS6

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2009

Availability

Academic Institutions and Nonprofits only

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pT2-cryR;clec14aCE1-basEgfp

Plasmid

#90140

Purpose

contains clec14a enhancer sequence and a basal promoter driving expression of eGFP

Depositor

Nathan Lawson

Article

Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

Insert

clec14aE1basEgfp

Use

Tags

EGFP

Expression

Bacterial

Mutation

Promoter

Available since

May 22, 2017

Availability

Academic Institutions and Nonprofits only

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PDE9A_pCSdest

Plasmid

#53944

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

PDE9A (PDE9A Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

SP6 and CMV

Available since

Sept. 8, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pCSDest2-SpCas9-NLS-3XHA-NLS

Plasmid

#69220

Purpose

Expresses wild type SpCas9 in mammalian cells

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

Insert

SpCas9

Use

CRISPR

Tags

NLS-3XHA-NLS

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pWS1806

Plasmid

#123047

Purpose

Melatonin GPCR

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

MTNR1A [3]

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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RWDD2B_pCSdest

Plasmid

#53866

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

RWDD2B (RWDD2B Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

SP6 and CMV

Available since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits
pWS033

Plasmid

#123056

Purpose

Green fluorescent protein

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

sfGFP [3]

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

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pE1B-C-F/Sox10-MCS3

Plasmid

#20242

Depositor

William Pavan

Article

Antonellis et al PLoS Genet. 2008 . 4(9):e1000174.

Insert

Sox10-MCS3

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2009

Availability

Academic Institutions and Nonprofits only

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TU#1450_unc-53a_cDNA

Plasmid

#82361

Purpose

express unc-53a cDNA

Depositor

Martin Chalfie

Article

Zheng et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

Insert

unc-53 a isoform (unc-53 Nematode)

Use

Tags

Expression

Worm

Mutation

Promoter

none

Available since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 GTF2H2D

Plasmid

#67081

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

GTF2H2D (GTF2H2C_2 Human)

Use

Cell-free expression

Tags

EGFP and His

Expression

Mutation

Promoter

T7

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only