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Showing: 10581 - 10600 of 14800 results

  1. pICE-HA-NLS-I-PpoI

    Plasmid
    #46963
    Purpose
    For constitutive or doxycycline-inducible expression of the site specific homing nuclease I-PpoI (human codon optimized). Confers resistance to puro. Use T-REx cells for doxy-inducible expression.
    Depositor
    Steve Jackson
    Article
    Britton et al J Cell Biol. 2013 Jul 29.
    Insert
    NLS-I-PpoI
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    CMV-TET
    Available since
    Aug. 20, 2013
    Availability
    Academic Institutions and Nonprofits only
    46963-plasmid-map-sequence-id-66854

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  9. pBabe TIR1-9myc

    Plasmid
    #47328
    Purpose
    For retroviral expression of E3 ubiquitin ligase osTIR1 with N-term NES and C-term 9x myc tag
    Depositor
    Don Cleveland
    Article
    Holland et al Proc Natl Acad Sci U S A. 2012 Dec 4;109
    Insert
    TRANSPORT INHIBITOR RESPONSE 1
    Use
    Retroviral
    Tags
    9x Myc and NES (nuclear export signal - ALQKKLEEL…
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 29, 2013
    Availability
    Academic Institutions and Nonprofits only
    47328-plasmid-map-sequence-id-108204

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  10. pEGFP mCherry-ApoB-EGFP

    Plasmid
    #112859
    Purpose
    Plasmid bearing the editing target of APOBEC1 fused between the mCherry cds and the EGFP one. In presence of APOBEC1, the CAA codon in ApoB is edited to a Stop codon (UAA), leading to loss of EGFP
    Depositor
    Silvestro Conticello
    Article
    Severi et al RNA Biol. 2015;12(4):389-97. doi: 10.108
    Insert
    mCherry-apoB-EGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV promoter
    Available since
    Oct. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    112859-plasmid-map-sequence-id-224731

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  13. ITCH

    Plasmid
    #36197
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    ITCH (ITCH Human)
    Use
    Tags
    His tag with TEV cleavage
    Expression
    Bacterial
    Mutation
    contains HECT domain
    Promoter
    Available since
    June 19, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  19. pET-6His-MBP-MpAgo

    Plasmid
    #79249
    Purpose
    This plasmid encodes the Argonaute protein from Marinitoga Piezophila and is codon optimized for E. coli expression
    Depositor
    Jennifer Doudna
    Article
    Kaya et al Proc Natl Acad Sci U S A. 2016 Mar 30. p
    Insert
    Marinitoga piezophila Argonaute
    Use
    CRISPR
    Tags
    6XHis-tag + MBP + PreScission Cleavage Site
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    July 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    79249-plasmid-map-sequence-id-151486

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Showing: 10581 - 10600 of 14800 results