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We narrowed to 48,743 results for: plasmid

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pGLIS3.1.0-gDNA

Plasmid

#113780

Purpose

CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor GLIS3

Depositor

Kevin White

Insert

GLIS3 (GLIS3 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pDEAF1.2.1-gDNA

Plasmid

#112449

Purpose

CRISPR plasmid for expression of Cas9 nickase (D10A) and gRNA targeting human transcription factor DEAF1

Depositor

Kevin White

Insert

DEAF1 (DEAF1 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pE2F5.1.0-gDNA

Plasmid

#112447

Purpose

CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor E2F5

Depositor

Kevin White

Insert

E2F5 (E2F5 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pDLX1.1.0-gDNA

Plasmid

#112439

Purpose

CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor DLX1

Depositor

Kevin White

Insert

DLX1 (DLX1 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pHBP1.1.0-gDNA

Plasmid

#112432

Purpose

CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor HBP1

Depositor

Kevin White

Insert

HBP1 (HBP1 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pATF1.1.0-gDNA

Plasmid

#112424

Purpose

CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ATF1

Depositor

Kevin White

Insert

ATF1 (ATF1 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pELK3.1.0-gDNA

Plasmid

#112418

Purpose

CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ELK3

Depositor

Kevin White

Insert

ELK3 (ELK3 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 6, 2018

Availability

Academic Institutions and Nonprofits only

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LIMK2 (LIMK2A-c052)

Plasmid

#110264

Purpose

Baculovirus expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

LIMK2 (LIMK2 Human)

Use

Tags

His-TEV

Expression

Insect

Mutation

Promoter

polyhedrin promoter

Available since

June 7, 2018

Availability

Academic Institutions and Nonprofits only

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pICE ADE2-MET17

Plasmid

#122885

Purpose

ADE2-MET17 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

Depositor

Randy Hampton

Article

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Tags

Expression

Yeast

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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pICE LYS2-LEU2

Plasmid

#122888

Purpose

LYS2-LEU2 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

Depositor

Randy Hampton

Article

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Tags

Expression

Yeast

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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pICE TRP1-HIS3

Plasmid

#122891

Purpose

TRP1-HIS3 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

Depositor

Randy Hampton

Article

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Tags

Expression

Yeast

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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pICE TRP1-MET17

Plasmid

#122893

Purpose

TRP1-MET17 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

Depositor

Randy Hampton

Article

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Tags

Expression

Yeast

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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EcNGRfEfC-R10S1B9

Bacterial Strain

#48895

Purpose

diversity was introduced via homologous recombination to 40 sites to improve fatty acid production. Sequencing of the strains at these sites is ongoing.

Depositor

George Church

Bacterial Resistance

Spectinomycin

Available since

Dec. 3, 2014

Availability

Academic Institutions and Nonprofits only
pAAV-hSyn-EGFP-KASH

Plasmid

#209201

Purpose

Neuronal expression of EGFP tagged KASH protein domain

Depositor

Larry Zweifel

Insert

eGFP-KASH

Use

AAV

Tags

KASH localization domain

Expression

Mammalian

Mutation

Promoter

hsyn

Available since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEXfrt-mCherry-KASH

Plasmid

#209200

Purpose

Flp-dependent expression of mCherry tagged KASH protein domain

Depositor

Larry Zweifel

Insert

mCherry-KASH

Use

AAV

Tags

KASH localization domain

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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SV40-eGFP-Z1

Plasmid

#127638

Purpose

Minimal backbone plasmid for mammalian eGFP expression from SV40 promoter with zeocin/bleocin bacterial resistance

Depositor

Jordan Green

Insert

eGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

SV40

Available since

May 28, 2020

Availability

Academic Institutions and Nonprofits only

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pCW57.1_TStrp_HA_Ct

Plasmid

#194066

Purpose

3rd generation, Inducible lentiviral expression, TRE-gateway; PGK-rtTA-2A-blasti

Depositor

Giulio Superti-Furga, RESOLUTE Consortium

Type

Empty backbone

Use

Lentiviral

Tags

Twin_Strep-HA

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pCW57.1_V5_FLAG_Ct

Plasmid

#194075

Purpose

3rd generation, Inducible lentiviral expression, TRE-gateway; PGK-rtTA-2A-blasti

Depositor

Giulio Superti-Furga, RESOLUTE Consortium

Type

Empty backbone

Use

Lentiviral

Tags

FLAG-V5

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 13, 2023

Availability

Academic Institutions and Nonprofits only

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FUW-hTie1-FLAG

Plasmid

#119233

Purpose

Plasmid encodes FLAG-tagged full-length human Tie1 gene cloned in lentiviral vector.

Depositor

Kari Alitalo

Insert

TIE1 (TIE1 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mutation

Promoter

P hUbC

Available since

Dec. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pUC119-MCS

Plasmid

#58807

Purpose

contains multiple cloning sites (MCSs) for cloning and transient expression of single or multiple guide RNA (gRNA) expression cassettes.

Depositor

Jen Sheen

Type

Empty backbone

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

July 22, 2014

Availability

Academic Institutions and Nonprofits only

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