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Addgene
Showing: 1041 - 1060 of 20514 results
  1. JDS246

    Plasmid
    #43861
    Purpose
    Expresses mammalian codon optimized Cas9 nuclease with C-term 3X FLAG from CMV and T7 promoters
    Depositor
    Insert
    mammalian codon-optimized streptococcus pyogenes Cas9 - 3X Flag
    Use
    CRISPR
    Tags
    3X FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
  2. PX458-AAVS1

    Plasmid
    #113194
    Purpose
    Encodes gRNA for human AAVS1
    Depositor
    Insert
    AAVS1 (PPP1R12C, AAVS1 Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Availability
    Academic Institutions and Nonprofits only
  3. pARID4B-donor

    Plasmid
    #132481
    Purpose
    CRISPR donor plasmid to create GFP fusion proteins
    Depositor
    Insert
    ARID4B (ARID4B Human)
    Use
    CRISPR
    Tags
    GFP
    Expression
    Mutation
    none
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  4. pCREB5-donor

    Plasmid
    #132521
    Purpose
    CRISPR donor plasmid to create GFP fusion proteins
    Depositor
    Insert
    CREB5 (CREB5 Human)
    Use
    CRISPR
    Tags
    GFP
    Expression
    Mutation
    none
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  5. pGRHL2-donor

    Plasmid
    #176351
    Purpose
    CRISPR donor plasmid to create GFP fusion proteins
    Insert
    GRHL2 (GRHL2 Human)
    Use
    CRISPR
    Tags
    GFP
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  6. pHDX-donor

    Plasmid
    #176353
    Purpose
    CRISPR donor plasmid to create GFP fusion proteins
    Insert
    HDX (HDX Human)
    Use
    CRISPR
    Tags
    GFP
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  7. pGMC00026 (aka pRC0462)

    Plasmid
    #195318
    Purpose
    Lentiviral guide RNA backbone derived from pSB700 with neomycin/G418
    Depositor
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  8. pGMC00018 (aka pRC0082)

    Plasmid
    #195320
    Purpose
    Minimal plasmid backbone with multiple cloning site for HDR donor DNA construction
    Depositor
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  9. pUC18T-mini-Tn7T-hph-dCas9

    Plasmid
    #218371
    Purpose
    Expression of dCas9 for CRISPR-interference
    Depositor
    Insert
    dCas9
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PtetA
    Availability
    Academic Institutions and Nonprofits only
  10. CMV-CASANOVA

    Plasmid
    #113035
    Purpose
    CASANOVA (AcrIIA4-LOV2 hybrid) expression in mammalian cells; enables optogenetic control of SpyCas9
    Depositor
    Insert
    CASANOVA (for CRISPR/Cas9 activity switching via a novel optogenetic variant of AcrIIA4)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  11. gRNA telo/GFP

    Plasmid
    #137713
    Purpose
    Vector co-expressing sgRNA telo and GFP
    Depositor
    Insert
    sgRNA telo + GFP
    Use
    AAV, CRISPR, and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6/RSV
    Availability
    Academic Institutions and Nonprofits only
  12. pBACH2.2.0-gDNA

    Plasmid
    #176375
    Purpose
    Expresses spCas9 and gRNA sequence targeting BACH2
    Insert
    BACH2 gRNA
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  13. pOTX2.1.0-gDNA

    Plasmid
    #176376
    Purpose
    Expresses spCas9 and gRNA sequence targeting OTX2
    Insert
    OTX2 gRNA
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
Showing: 1041 - 1060 of 20514 results