Addgene

We narrowed to 3,034 results for: aaas

Showing: 1041 - 1060 of 3034 results

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PX458 pSp Cas9 Rhino1 tagA

Plasmid

#211627

Purpose

Cas9 Rhino1 tagA

Depositor

Agnel Sfeir

Article

Brambati et al Science. 2023 Aug 11;381(6658):653-660.

Insert

RHNO tagA

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pMLS1272

Plasmid

#188891

Purpose

Ric-4 sgRNA expression vector

Depositor

Erik Jorgensen

Article

Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

Insert

PU6(R07E5.16)::Ric-4 sgRNA (CELE_Y22F5A.3 Nematode)

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pLeGO.sgGata1.4.RUNX1A.iG2

Plasmid

#181976

Purpose

gene knock out of mouse GATA1, overexpression of 3xFLAG-RUNX1A (human)

Depositor

Jan-Henning Klusmann

Inserts

GATA1 gRNA: CCTAGACCAGGAAAATCCAT (Gata1 Mouse)
RUNX1A (RUNX1 Human)

Use

Lentiviral

Tags

HA

Expression

Mutation

Promoter

Available since

April 26, 2022

Availability

Academic Institutions and Nonprofits only

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PLKO.1-UPF3B-3'UTR

Plasmid

#136044

Purpose

UPF3B shRNA (Targeting 3'UTR) inserted into the PLKO.1 plasmid (CAGGGCAAAGAATAGAGAGAA)

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

UPF3B (UPF3B Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pALPS puro miR30-TAK1

Plasmid

#117162

Purpose

Target site AGCGCCCTTCAATGGAGGAAAT

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

shRNA targeting miR30

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pSV_229

Plasmid

#122934

Purpose

Expression of the marked mRNA under the control of substitutional promoters.

Depositor

Attila Becskei

Article

Baudrimont et al Sci Adv. 2017 Jul 12;3(7):e1700006. doi:

Insert

YBR026C (Marked)

Use

Tags

Expression

Yeast

Mutation

YBR026C: bp 52-63 AAGCACTTCAAA to AAACATTTTAAG

Promoter

[tetO2]4inPgal1

Available since

April 9, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-EPN-01(ΔL1+L2)

Plasmid

#80051

Purpose

Expression of EPN-01 mutated in both L domains

Depositor

Wesley Sundquist

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

EPN-01(deltaPTAP/deltaYP)

Use

Tags

Myc

Expression

Mammalian

Mutation

PTAP10AAAA, YP37AA

Promoter

CMV

Available since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pFUGW-hGtACR2-EYFP

Plasmid

#67877

Purpose

Mammalian expression construct of human codon-adapted anion channelrhodopsin 2 from Guillardia theta fused in frame to EYFP via a NotI site in the pFUGW vector backbone

Depositor

John Spudich

Article

Govorunova et al Science. 2015 Jun 25. pii: aaa7484.

Insert

hGtACR2

Use

Lentiviral

Tags

EYFP

Expression

Mammalian

Mutation

Promoter

UbC

Available since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pLKO-PTEN-shRNA-3001

Plasmid

#25639

Depositor

Todd Waldman

Article

Kim et al Mol Cell Biol. 2007 Jan . 27(2):662-77.

Insert

PTEN-shRNA-3001 (PTEN Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 21, 2010

Availability

Academic Institutions and Nonprofits only

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pColE1_sgRNA_M1S_AmpR

Plasmid

#119153

Purpose

Encodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR with one mismatch in the seed region

Depositor

Vincent Noireaux

Article

Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

Insert

sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, with one mismatch in the seed region

Use

Crispr

Tags

Expression

Mutation

Promoter

J23119

Available since

Dec. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pColE1_sgRNA_M1NS_AmpR

Plasmid

#119155

Purpose

Encodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR with one mismatch in the non-seed region

Depositor

Vincent Noireaux

Article

Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

Insert

sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, with one mismatch in the non-seed region

Use

Crispr

Tags

Expression

Mutation

Promoter

J23119

Available since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pFUGW-hGtACR1-EYFP

Plasmid

#67795

Purpose

Mammalian expression construct of human codon-adapted anion channelrhodopsin 1 from Guillardia theta fused in frame to EYFP via a NotI site in the pFUGW vector backbone

Depositor

John Spudich

Article

Govorunova et al Science. 2015 Jun 25. pii: aaa7484.

Insert

hGtACR1

Use

Lentiviral

Tags

EYFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pX459_gRNA pAS single_hspCas9

Plasmid

#193311

Purpose

Cas9 from S. pyogenes and U6-pAS single sgRNA (V2.0)

Depositor

Simon Elsaesser

Article

Meineke et al Methods Mol Biol. 2023;2676:169-180. doi

Insert

Cas9 from S. pyogenes and U6-pAS single sgRNA (V2.0)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

not applicable

Promoter

Available since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pENTR-PpU6P-sgRNA-L1L2

Plasmid

#113735

Purpose

Gateway entry vector containing attL1 and attL2 sites, U6 promoter, BsaI sites for protospacer ligation, and sgRNA.

Depositor

Magdalena Bezanilla

Article

Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

Insert

PpU6P::sgRNA

Use

CRISPR; Gateway entry vector

Tags

Expression

Mutation

Promoter

P. patens U6 promoter

Available since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pgRNAtet-phaZ

Plasmid

#169874

Purpose

Single guide RNA plasmid for Cas9 targeting phaZ in P. putida

Depositor

Brian Pfleger

Article

Cook et al Metab Eng. 2021 Jun 24;67:112-124. doi:

Insert

sgRNA towards phaZ in Pseudomonas putida

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 9, 2021

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR - DDX3Y sgRNA 2

Plasmid

#70657

Purpose

Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a DDX3Y targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

Depositor

David Sabatini

Article

Wang et al Science. 2015 Oct 15. pii: aac7041.

Insert

sgRNA against DDX3Y

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 29, 2015

Availability

Academic Institutions and Nonprofits only
pColE1_sgRNA_M2S_AmpR

Plasmid

#119154

Purpose

Encodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR with two mismatches in the seed region

Depositor

Vincent Noireaux

Article

Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

Insert

sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, with two mismatches in the seed region

Use

Crispr

Tags

Expression

Mutation

Promoter

J23119

Available since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pSuper.Retro.Puro-WDR5 shRNA 2

Plasmid

#59975

Purpose

Knockdown of WDR5

Depositor

Debu Chakravarti

Article

Kim et al Mol Cell. 2014 May 22;54(4):613-25. doi:

Insert

WDR5 shRNA

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 8, 2014

Availability

Academic Institutions and Nonprofits only
pTX1-SMN1-exon7-ESE1

Plasmid

#126041

Purpose

In-vitro-transcription of SMN1-exon7-ESE1 RNA in NMR tube for "Systems NMR" analysis

Depositor

Frédéric Allain

Article

Nikolaev et al Nat Methods. 2019 Aug;16(8):743-749. doi

Insert

SMN1 exon7 ESE1

Use

Tags

Expression

Mutation

Promoter

Available since

May 25, 2021

Availability

Academic Institutions and Nonprofits only

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pLKO.1 EB1 shRNA #1

Plasmid

#37928

Depositor

Torsten Wittmann

Article

Gierke et al Curr Biol. 2012 May 8;22(9):753-62. Epub

Insert

EB1 shRNA #1 (MAPRE1 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

human U6

Available since

July 13, 2012

Availability

Academic Institutions and Nonprofits only

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