Addgene

We narrowed to 15,233 results for: LEA

Showing: 1041 - 1060 of 15233 results

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pDZ585 pKAN 2x-mCherry

Plasmid

#72236

Purpose

Floxed Kanamycin (G418) selection maker; 3' tagging vector used to create C-terminal fusion proteins with 2x-mCherry

Depositor

Daniel Zenklusen

Article

Saroufim et al J Cell Biol. 2015 Dec 21;211(6):1131-40.

Insert

2x-mCherry

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 8, 2016

Availability

Academic Institutions and Nonprofits only

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H2B-GFP-AsLOV2-NES27 (pDN158)

Plasmid

#72659

Purpose

Constitutively nuclear variant of a very strong, photocaged NES (AsLOV2-NES 27) for blocking endogenous, CRM-1-dependent nuclear export

Depositor

Roland Eils, Barbara Di Ventura

Article

Niopek et al Nat Commun. 2016 Feb 8;7:10624. doi: 10.

Insert

H2B-GFP-AsLOV2-NES27

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pREP3-TEV-NLS

Plasmid

#21146

Depositor

Stephen Kearsey

Article

Yang et al BMC Mol Biol. 2005 . 6():13.

Insert

TEV protease (TEVgp1 tobacco etch virus)

Use

S. pombe

Tags

2xNLS, NLS, and myc

Expression

Yeast

Mutation

Promoter

Available since

July 6, 2009

Availability

Academic Institutions and Nonprofits only

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Banshee-Sh-Ctrl

Plasmid

#85587

Purpose

Control hairpin sequence used in gene knockdown experiements

Depositor

Alex Minella

Article

Swartz et al Mol Cell Biol. 2016 Oct 17. pii: MCB.002

Insert

sh-Ctrl

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

H1

Available since

Feb. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pDZ514 pURA MET25p PP7-PS-2x-yeGFP

Plasmid

#72230

Purpose

Uracil selection; Expression of PP7-2x-yeGFP fusion protein under the MET25 promoter

Depositor

Daniel Zenklusen

Article

Saroufim et al J Cell Biol. 2015 Dec 21;211(6):1131-40.

Insert

PP7-PS-2x-yeGFP

Use

Tags

Expression

Yeast

Mutation

Promoter

MET25

Available since

Aug. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pDZ536 pURA ADE3p PP7-PS-2x-yeGFP

Plasmid

#72234

Purpose

Uracil selection; Expression of PP7-2x-yeGFP fusion protein under the ADE3 promoter

Depositor

Daniel Zenklusen

Article

Saroufim et al J Cell Biol. 2015 Dec 21;211(6):1131-40.

Insert

PP7-PS-2x-yeGFP

Use

Tags

Expression

Yeast

Mutation

Promoter

ADE3p

Available since

Aug. 8, 2016

Availability

Academic Institutions and Nonprofits only

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GPKAnes

Plasmid

#61092

Purpose

GFP-tagged PKA catalytic subunit targeted to the extranuclear cytoplasm by addition of a nuclear export signal

Depositor

Steven Green

Article

Bok et al J Neurosci. 2003 Feb 1;23(3):777-87.

Insert

pkac2

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only
pLenti-CMV-SMCHD1-R1868*-3xFLAG

Plasmid

#130679

Purpose

Expresses human SMCHD1 R1868* with a C-terminal 3xFLAG tag

Depositor

Stephen Tapscott

Article

Hiramuki et al Skelet Muscle. 2018 Aug 2;8(1):24. doi:

Insert

SMCHD1 R1868* (SMCHD1 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

R1868_V2005delinsX

Promoter

CMV

Available since

June 22, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-SMCHD1-Exon37-48-3xFLAG

Plasmid

#130688

Purpose

Expresses human SMCHD1 Exon37-48 with a C-terminal 3xFLAG tag

Depositor

Stephen Tapscott

Article

Hiramuki et al Skelet Muscle. 2018 Aug 2;8(1):24. doi:

Insert

SMCHD1 Exon37-48 (SMCHD1 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

1M_T1522delinsM

Promoter

CMV

Available since

Sept. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-SMCHD1-Exon1-36-3xFLAG

Plasmid

#130681

Purpose

Expresses human SMCHD1 Exon1-36 with a C-terminal 3xFLAG tag

Depositor

Stephen Tapscott

Article

Hiramuki et al Skelet Muscle. 2018 Aug 2;8(1):24. doi:

Insert

SMCHD1 Exon1-36 (SMCHD1 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

D1523_V2005delinsX

Promoter

CMV

Available since

Sept. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pDESTFLAGHApuro3-Ago Chi-a

Plasmid

#14075

Depositor

Thomas Tuschl

Article

Yuan et al Mol Cell. 2005 Aug 5. 19(3):405-19.

Insert

Ago Chi-a

Use

Tags

FLAG/HA

Expression

Mammalian

Mutation

Ago Chimera-a

Promoter

Available since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only
pDZ529 pTRP MET25p PP7-PS-2x-yeGFP

Plasmid

#72233

Purpose

Tryptophan selection; Expression of PP7-2x-yeGFP fusion protein under the MET25 promoter

Depositor

Daniel Zenklusen

Article

Saroufim et al J Cell Biol. 2015 Dec 21;211(6):1131-40.

Insert

PP7-PS-2x-yeGFP

Use

Tags

Expression

Yeast

Mutation

Promoter

MET25

Available since

Aug. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pDESTFLAGHApuro3-Ago Chi-b

Plasmid

#14085

Depositor

Thomas Tuschl

Article

Yuan et al Mol Cell. 2005 Aug 5. 19(3):405-19.

Insert

Ago Chi-b

Use

Tags

FLAG/HA

Expression

Mammalian

Mutation

Ago Chimera-b

Promoter

Available since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only
pDESTFLAGHApuro3-Ago Chi-c

Plasmid

#14086

Depositor

Thomas Tuschl

Article

Yuan et al Mol Cell. 2005 Aug 5. 19(3):405-19.

Insert

Ago Chi-c

Use

Tags

FLAG/HA

Expression

Mammalian

Mutation

Ago Chimera-c

Promoter

Available since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only
pDESTFLAGHApuro3-Ago Chi-d

Plasmid

#14087

Depositor

Thomas Tuschl

Article

Yuan et al Mol Cell. 2005 Aug 5. 19(3):405-19.

Insert

Ago Chi-d

Use

Tags

FLAG/HA

Expression

Mammalian

Mutation

Ago Chimera-d

Promoter

Available since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only
pET-28a(+)-T7-OmpA-MNaseA-6xHis

Plasmid

#214808

Purpose

Expression of 6xhis-tagged micrococcal nuclease A (MNaseA)

Depositor

Peter Sarin

Article

Gregorova et al J Biol Chem. 2024 Nov 27;301(1):108020.

Insert

OmpA-Micrococcal nuclease A

Use

Tags

6xhis

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

Nov. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pET-28a(+)-T7-OmpA-MNaseB-6xHis

Plasmid

#214809

Purpose

Expression of 6xhis-tagged micrococcal nuclease B (MNaseB)

Depositor

Peter Sarin

Article

Gregorova et al J Biol Chem. 2024 Nov 27;301(1):108020.

Insert

OmpA-Micrococcal nuclease B

Use

Tags

6xhis

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

Nov. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pEJS2696-CMV-Nme2SmuCas9-D56R

Plasmid

#228717

Purpose

Expresses Nme2Smu Cas9 nuclease with D56R mutation in mammalian cells

Depositor

Erik Sontheimer

Article

Bamidele et al bioRxiv November 07, 2024

Insert

Nme2SmuCas9-D56R nuclease

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Nme2-Cas9, Smu PID, D56R

Promoter

CMV

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pEJS2697-CMV-Nme2SmuCas9-E932R

Plasmid

#228718

Purpose

Expresses Nme2Smu Cas9 nuclease with E932R mutation in mammalian cells

Depositor

Erik Sontheimer

Article

Bamidele et al bioRxiv November 07, 2024

Insert

Nme2SmuCas9-E932R nuclease

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Nme2-Cas9, Smu PID, E932R

Promoter

CMV

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCL1177

Plasmid

#217961

Purpose

Dual cistronic expression vector for the RAPID-release approach. H3.2-mCherry is released from mitochondria to the nucleus upon addition of rapamycin.

Depositor

Andrew Bowman

Article

Pardal et al Elife. 2022 Sep 6;11:e81755. doi: 10.755

Insert

Histone H3 (HIST2H3PS2 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 18, 2024

Availability

Academic Institutions and Nonprofits only

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