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pCellFree_G03 PSMC1

Plasmid

#67087

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

PSMC1 (PSMC1 Human)

Use

Cell-free expression

Tags

EGFP and His

Expression

Mutation

E275G, I287V and R294G

Promoter

T7

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pME-Fv2E-flt4um200-ns

Plasmid

#67696

Purpose

middle entry plasmid with dimerizable extracellular domain fused to um200 mutant Danio Flt4 cytoplasmic domain

Depositor

Nathan Lawson

Article

Shin et al Development. 2016 Oct 15;143(20):3785-37

Insert

Fv2E-flt4um200

Use

Tags

HA

Expression

Mutation

um200, no stop codon; N1056S

Promoter

Available since

Oct. 22, 2015

Availability

Academic Institutions and Nonprofits only

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pHAGE-eGFP-TAX1BP1 CC3D

Plasmid

#175776

Purpose

Lentiviral vector expressing N-terminally tagged eGFP-TAX1BP1 CC3D

Depositor

Wade Harper

Article

Eapen et al Elife. 2021 Sep 29;10. pii: 72328. doi:

Insert

TAX1BP1 (TAX1BP1 Human)

Use

Lentiviral

Tags

Expression

Mutation

CC3D

Promoter

Available since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE-eGFP-TAX1BP1 CC2D

Plasmid

#175775

Purpose

Lentiviral vector expressing N-terminally tagged eGFP-TAX1BP1 CC2D

Depositor

Wade Harper

Article

Eapen et al Elife. 2021 Sep 29;10. pii: 72328. doi:

Insert

TAX1BP1 (TAX1BP1 Human)

Use

Lentiviral

Tags

Expression

Mutation

CC2D

Promoter

Available since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE-eGFP-TAX1BP1 CC1D

Plasmid

#175774

Purpose

Lentiviral vector expressing N-terminally tagged eGFP-TAX1BP1 CC1D

Depositor

Wade Harper

Article

Eapen et al Elife. 2021 Sep 29;10. pii: 72328. doi:

Insert

TAX1BP1 (TAX1BP1 Human)

Use

Lentiviral

Tags

Expression

Mutation

CC1D

Promoter

Available since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pCMB-TMEg

Plasmid

#61209

Purpose

Fluorescent reporter for plasma membrane and trunk peri-arbuscular membrane MtBCPsp-GFP-BCP expressed under MtBCP1 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

MtBCPsp-GFP-BCP

Use

Tags

EGFP

Expression

Plant

Mutation

Promoter

MtBCP1

Available since

April 10, 2015

Availability

Academic Institutions and Nonprofits only

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pDEST51-LRRK2-R1441H

Plasmid

#25083

Depositor

Mark Cookson

Article

J Biol Chem. 2008 Jun 13;283(24):16906-1

Insert

LRRK2 (LRRK2 Human)

Use

Destination vector

Tags

6XHis and V5

Expression

Mammalian

Mutation

Arg 1441 mutated to His

Promoter

Available since

June 17, 2010

Availability

Academic Institutions and Nonprofits only
pE1B-C-F/Sox10-MCS8

Plasmid

#20247

Depositor

William Pavan

Article

Antonellis et al PLoS Genet. 2008 . 4(9):e1000174.

Insert

Sox10-MCS8

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2009

Availability

Academic Institutions and Nonprofits only

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pHWBF06exp

Plasmid

#130831

Purpose

Expression of plant LINC fluorescent fusion protein for cell biology

Depositor

Hank Bass

Article

Gumber et al J Cell Sci. 2019 Feb 11;132(3). pii: jcs

Insert

SUN2

Use

Tags

mCherry-FLAG-HA

Expression

Plant

Mutation

Promoter

CaMV 35S

Available since

Feb. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pWS788

Plasmid

#123059

Purpose

_-factor protease

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

Bar1 [3]

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

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HS-aPKC-CAAX-mKate2

Plasmid

#105950

Purpose

heat shock inducible transgenesis

Depositor

Caren Norden

Article

Strzyz et al Dev Cell. 2015 Jan 26;32(2):203-19. doi:

Insert

aPKC-CAAX

Use

Tags

mKate2

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 14, 2018

Availability

Academic Institutions and Nonprofits only

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SETD4_pCSdest

Plasmid

#53814

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

SETD4 (SETD4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

SP6 and CMV

Available since

Sept. 9, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pDEST-APIC-Flp1

Plasmid

#112806

Purpose

gateway cloning destination vector to express enhancer driven Flip1 in Drosophila

Depositor

Chun Han

Article

Poe et al Proc Natl Acad Sci U S A. 2017 Sep 19;11

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/nV5-DEST-Apr1

Plasmid

#20135

Depositor

Valeri Vasioukhin

Article

Lien et al J Cell Biol. 2008 Dec 15. 183(6):989-97.

Insert

pcDNA3.1/nV5-DEST-Arp1 (Actr1a Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only
pT2-cryR;mafbbCE1-P1Egfp

Plasmid

#90155

Purpose

contains mafbb enhancer sequence, and mafbb specific gene promoter driving expression of eGFP

Depositor

Nathan Lawson

Article

Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

Insert

mafbbE1P1Egfp

Use

Tags

EGFP

Expression

Bacterial

Mutation

Promoter

Available since

June 29, 2017

Availability

Academic Institutions and Nonprofits only

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pAW-EGFP-4xlet-7 (pAW-AS27)

Plasmid

#40834

Purpose

miRNA sensor used in Drosophila cell lines

Depositor

Phillip Zamore

Article

Ameres et al Science. 2010 Jun 18;328(5985):1534-9.

Insert

Four target sites perfectly complementary to let-7

Use

Mirna reporter

Tags

EGFP

Expression

Insect

Mutation

Promoter

Actin5C

Available since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only
pMT-GFP-Sas4

Plasmid

#31756

Depositor

Ron Vale

Article

Goodwin et al Cell. 2010 Oct 15;143(2):263-74.

Insert

Sas4 (Sas-4 Fly)

Use

Tags

GFP

Expression

Insect

Mutation

N70T

Promoter

Metallothionein

Available since

Jan. 20, 2012

Availability

Academic Institutions and Nonprofits only
pE1B-C-F/Sox10-MCS1B

Plasmid

#20239

Depositor

William Pavan

Article

Antonellis et al PLoS Genet. 2008 . 4(9):e1000174.

Insert

Sox10-MCS1B

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2009

Availability

Academic Institutions and Nonprofits only

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pDEST_TO_TAF15

Plasmid

#26378

Depositor

Thomas Tuschl

Article

Hoell et al Nat Struct Mol Biol. 2011 Nov 13. doi: 1

Insert

TAF15 (TAF15 Human)

Use

Tags

FLAG/HA

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 22, 2010

Availability

Academic Institutions and Nonprofits only
pSLIK TT 3xFLAG LacZ neo

Plasmid

#83105

Purpose

Lentiviral vector for inducible expression of LacZ with 3xFLAG N-terminal tag

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

LacZ

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only