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Plasmid#166788PurposeInsect cell expression vector for DPP9L (protein purification)DepositorInsertDPP9 (DPP9 Human)
UseTagsHIS-TEVExpressionInsectMutationPromoterpolyhedrinAvailable sinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFastBac HTB DPP9S
Plasmid#166787PurposeInsect cell expression vector for DPP9S (protein purification)DepositorInsertDPP9 (DPP9 Human)
UseTagsHIS-TEVExpressionInsectMutationPromoterpolyhedrinAvailable sinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFastBac HTB DPP9S S730A
Plasmid#169946PurposePurification of catalytically-dead DPP9S from insect cellsDepositorInsertDPP9 (DPP9 Human)
UseTagsHIS-TEVExpressionInsectMutationS730APromoterpolyhedrinAvailable sinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBC2T-lamin
Plasmid#108972Purposeexpresses BC2-tag-lamin in mammalian cellsDepositorInsertlamin B1 (LMNB1 Human)
UseTagsBC2-tagExpressionMammalianMutationPromoterAvailable sinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFastBac HTB DPP9S D808N
Plasmid#169947PurposePurification of catalytically-dead DPP9S from insect cellsDepositorInsertDPP9 (DPP9 Human)
UseTagsHIS-TEVExpressionInsectMutationD808NPromoterpolyhedrinAvailable sinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pQCXIP GFP-MOSPD2 RD/LD
Plasmid#186468PurposeExpression of human MOSPD2 (mutant R404D/L406D, unable to bind FFAT motifs) fused to EGFP in mammalian cellsDepositorInsertMOSPD2 motile sperm domain containing 2 (MOSPD2 Human)
UseRetroviralTagsEGFPExpressionMammalianMutationR404D/L406D (mutant unable to bind FFAT motifs)PromoterAvailable sinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCSIIbleo-PIF3-mEGFP
Plasmid#139483PurposeExpresses PIF3-mEGFP in mammalian cells.DepositorInsertPIF3 (PIF3 Mustard Weed)
UseLentiviralTagsmEGFPExpressionMammalianMutationPIF3 1-100 aaPromoterHuman EF1a promoterAvailable sinceApril 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSF3-Flag-Cbir-Cdc25C S216A_Myc-Nbir-14-3-3e
Plasmid#90011PurposeSplit-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-Cdc25C S216A and N-terminally tagged NBir[2-256]-14-3-3eDepositorInsertsCBirA*-Cdc25C S216A
NBirA*-14-3-3e
UseRetroviral; Flp/frtTagsFLAG and MycExpressionMammalianMutationSer 216 to AlaPromoterCMVAvailable sinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBC2T-LC3B
Plasmid#112026Purposeexpresses BC2-tag-LC3B in mammalian cellsDepositorInsertLC3B (MAP1LC3B Human)
UseTagsBC2-tagExpressionMammalianMutationPromoterAvailable sinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pClneoFLAG human Dendrin
Plasmid#47934Purposeexpresses FLAG tagged human Dendrin in mammalian cellsDepositorInsertDendrin-1 (DDN Human)
UseTagsFLAGExpressionMammalianMutationcontains amino acids 2-711 of human DendrinPromoterCMVAvailable sinceSept. 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
eSpCas9-EF-5Usp5
Plasmid#174873PurposeCRISPR vector for generating Usp5 STREAMING-tag KI cellDepositorInsertsgRNA for mouse Usp5 (Usp5 Synthetic)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
eSpCas9-EF-5Wnk1
Plasmid#174874PurposeCRISPR vector for generating Wnk1 STREAMING-tag KI cellDepositorInsertsgRNA for mouse Wnk1 (Wnk1 Synthetic)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-FLAG-Co-NF-kB-Cterm
Plasmid#180199PurposeExpresses FLAG-tagged Capsaspora NF-kB C-terminal sequences in mammalian cellsDepositorInsertCo-NF-kBCterm
UseTagsFLAG-tagged fusion proteinExpressionMammalianMutationaa 542-1224PromoterCMVAvailable sinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
Halo-WIPI2B H85E K88E I92E
Plasmid#175033PurposeExpresses WIPI2B (H85E K88E I92E) fused to Halo in mammalian cells to abrogate interaction with ATG16L1DepositorInsertWIPI2B (H85E K88E I92E) (WIPI2 Human)
UseTagsHaloTagExpressionMammalianMutationHistadine 85 to Glutamate (CAC to GAG); Lysine 88…PromoterCMVAvailable sinceDec. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pETDuet1-MBP-NAAEF-PNR(217-410 C275)-His6
Plasmid#177849PurposeBacterial Expression of PNRDepositorInsertphotoreceptor cell-specific nuclear receptor (NR2E3 Human)
UseTags6xHis and MBPExpressionBacterialMutationPromoterAvailable sinceDec. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
FLAG-HA-RNF68 [dRING]
Plasmid#126549PurposeExpresses FLAG-HA-tagged RNF68 [dRING mutant] in mammalian cellsDepositorInsertRNF68 (PCGF1 Human)
UseRetroviralTagsFLAG-HAExpressionMammalianMutationRING domain deletion mutant [dRING mutation is a …PromoterLTRAvailable sinceJuly 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO h-Stx3S-2xMyc/His
Plasmid#99744PurposeExpresses human Stx3S with a C-term myc-myc-his tag in mammalian cells.DepositorInsertStx3S (STX3 Human)
UseTagsmyc-myc-hisExpressionMammalianMutationPromoterCMVAvailable sinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMCh-1539
Plasmid#82432PurposePlasmid for expression of GST-tagged human-Nematostella GW182 chimera in mammalian cellsDepositorInserths-nvGW182 chimera
UseTagsGSTExpressionMammalianMutationaa 1-1169 of siRNA resistant hsTNRC6A (Q8NDV7-2) …PromoterEF1AAvailable sinceSept. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMCh-1537
Plasmid#82430PurposePlasmid for expression of NHA-tagged human-Nematostella GW182 chimera in mammalian cellsDepositorInserths-nvGW182 chimera
UseTagsNHAExpressionMammalianMutationaa 1-1169 of siRNA resistant hsTNRC6A (Q8NDV7-2) …PromoterCMVAvailable sinceSept. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
CXCR2-DuET
Plasmid#213219PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertCXCR2 (CXCR2 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only