-
Plasmid#127926PurposeTarget plasmid containing PSP1DepositorInsertPSP1 target (GGTT PAM)
UseTagsExpressionMutationPromoterAvailable sinceJuly 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3_GLP1R-NTEV-TCS-GV-2xHA
Plasmid#194378PurposeSplit TEV assaysDepositorInsertGLP1R-NTEV-TCS-GV-2xHA (GLP1R Human, Synthetic)
UseTags2xHAExpressionMammalianMutationPromoterCMVAvailable sinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
rEcoli XpS
Bacterial Strain#192872PurposeMG1655-C321 -- All 321 UAG codons changed to UAADepositorBacterial ResistanceBleocin (zeocin)SpeciesEscherichia coliAvailable sinceDec. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET21a-HisRS-His
Plasmid#124111PurposeBacterial expression plasmid for PURE system componentsDepositorInsertHistidyl-tRNA ligase (hisS Escherichia coli)
UseTags6x-HisExpressionBacterialMutationPromoterAvailable sinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCB301TOR-GFP
Plasmid#164799PurposeExpression of GFP in oomycetes using Agrobacterium-mediated transformationDepositorInsertGFP
UseOomycete expressionTagsExpressionMutationPromoterAvailable sinceMarch 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-Lifeact_Halo_T2A_EGFP
Plasmid#135445PurposeMamalian cell expression of HaloTag7 localized at F-actin and cytosolic EGFPDepositorInsertLifact-HaloTag7 and EGFP
UseTagsLifactExpressionMammalianMutationPromoterCMV/TetOAvailable sinceJan. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
-
pMCB306-GFP-Rab12
Plasmid#198471Purposelentiviral expression of GFP-Rab12DepositorInsertGFP-Rab12 (RAB12 Human)
UseLentiviralTagsGFPExpressionMammalianMutationPromoterEF1AAvailable sinceApril 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
Nup133_mEGFP(-8a)
Plasmid#163417PurposeMammalian expression of Nup133 fused at its carboxyl-terminus to mEGFP with no linker and a total net fusion of (-8 amino acids)DepositorInsertNup133 (NUP133 Human)
UseTagsmEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pQE80L-IL2 C125S
Plasmid#104348PurposeExpresses human IL-2 (C125S)DepositorInsertHuman IL-2 (C125S) (IL2 Human)
UseTagsMRGSHHHHHGS-tagExpressionBacterialMutationC125S mutation (TGT -> TCC)PromoterT5Available sinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pIRES2-eGFP:HA-mTREK-1
Plasmid#224773PurposeMammalian expression vector for generating mouse TREK-1DepositorInsertPotassium channel subfamily K member 2 (Kcnk2 Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHAGE TRE dCas9-VP64
Plasmid#50916PurposeTet-regulatable dCas9-VP64 2nd generation lentiviral expression vectorDepositorInsertdCas9
UseCRISPR and LentiviralTags3xHA and VP64ExpressionMammalianMutationD10A, H840A nuclease-deficientPromoterTREAvailable sinceMarch 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-CMV-Rheb(S16H)-IRES-eGFP-W
Plasmid#32520DepositorInsertConstitutively active Rheb (S16H) (Rheb Rat)
UseLentiviralTagsExpressionMammalianMutationS16H to make constitutively activePromoterCMVAvailable sinceSept. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-FlaviA-mNeptune-puro
Plasmid#140091PurposeFluorescence activatable reporter of flavivirus NS2B-NS3 protease activityDepositorInsertFlaviA-mNeptune
UseLentiviral; High expressionTagsmNeptuneExpressionMammalianMutationPromoterCMVAvailable sinceApril 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-ABE8.20m-nSpCas9-NG-P2A-EGFP (KAC1164)
Plasmid#185919PurposepCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nickase SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFPDepositorInserthuman codon optimized ABE8.20m-nSpCas9-NG-P2A-EGFP
UseIn vitro transcription; t7 promoterTagsBPNLS and BPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationnSpCas9-NG=D10A/L1111R/D1135V/G1218R/E1219F/A1322…PromoterCMV and T7Available sinceJuly 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCXLE-hMLN
Plasmid#27079PurposeNon-integrating (episomal) expression of human C-MYC, LIN28 and NANOGDepositorInsertC-MYC, LIN28, NANOG (MYC Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
pUC18T-mini-Tn7T-Tp-ecfp
Plasmid#65034PurposeTpR mini-Tn7 vector, mobilizable, for CFP tagging bacteriaDepositorInsertecfp
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDHt/sk-PC
Plasmid#92125PurposeExpression of codon-optimized Cas9 with Ppdc promoter in Trichoderma reeseiDepositorInsertCas9
UseCRISPR; Trichoderma reesei expressionTagsExpressionMutationPromoterPpdcAvailable sinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDB5320
Plasmid#204830PurposeAn integrating plasmid containing both the inducible enotetSW2 promoter and the coding sequence of the TetR repressor under the control of the constitutive CMV promoter.DepositorInsertstetR
mECitrine
UseTagsExpressionYeastMutationPromoterCMV promoter and enotetSW2 promoterAvailable sinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDB5319
Plasmid#204829PurposeAn integrating plasmid containing both the inducible enotetSW1 promoter and the coding sequence of the TetR repressor under the control of the constitutive CMV promoter.DepositorInsertstetR
mECitrine
UseTagsExpressionYeastMutationPromoterCMV promoter and enotetSW1 promoterAvailable sinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only