Skip to main content
Addgene
Showing: 10481 - 10500 of 26211 results

  10. pDGB3 alpha1 U6-26:gRNA 4 (Pnos):2.1 aptamer (GB1724)

    Plasmid
    #160621
    Purpose
    Target for the Nopaline Synthetase Promoter with the MS2 recognition 2.1 loop in position3' in the scaffold
    Depositor
    Diego Orzaez
    Article
    Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-
    Insert
    U6-26:gRNA 4 (pNos): 2.1 aptamer
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    U6-26
    Available since
    Jan. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    160621-plasmid-map-sequence-id-322006

    Enlarge

  11. pCBh_3x Flag-NLS-OsCas12f1-NLS_pA_phU6_gRNA scaffold-spacer_pCMV_mCherry_pA

    Plasmid
    #197024
    Purpose
    Vector encoding a human codon-optimized OsCas12f1 driven by CBh promoter, guide RNAs compatible with OsCas12f1 driven by hU6, and mCherry driven by CMV promoter
    Depositor
    Xiangfeng Kong
    Article
    Kong et al Nat Commun. 2023 Apr 11;14(1):2046. doi:
    Insert
    humanized OsCas12f1
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CBh, CMV, hU6
    Available since
    May 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    197024-plasmid-map-sequence-id-388957

    Enlarge

  14. LF701: pMAGIC (L1-R5) hU6::xCas9(3.7) gRNA scaffold

    Plasmid
    #121817
    Purpose
    pMAGIC L1-R5 entry plasmid, contains empty human U6-driven xCas9(3.7) (i.e. SpCas9) gRNA scaffold for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Type
    Empty backbone
    Use
    Synthetic Biology; Pmagic gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121817-plasmid-map-sequence-id-237365

    Enlarge

  16. pJEP315-pAAV-U6SaCas9gRNA(CREB)-CMV-SaCas9-DIO-pA

    Plasmid
    #113692
    Purpose
    U6 SaCas9 gRNA expression cassette containing a gRNA targeting the CREB gene, followed by a CMV driven inverted SaCas9. SaCas9 is floxed to render the system cre-dependent.
    Depositor
    Jonathan Ploski
    Article
    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.
    Inserts
    SaCas9 (NEWENTRY )
    SaCas9 gRNA Cassette (NEWENTRY )
    Use
    AAV, CRISPR, Cre/Lox, and Mouse Targeting
    Tags
    NLS
    Expression
    Mutation
    Promoter
    Cytomegalo Virus(CMV)
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    113692-plasmid-map-sequence-id-229557

    Enlarge

  20. LG002: pMAGIC (L1-R5) mU6::xCas9(3.7) gRNA scaffold

    Plasmid
    #121816
    Purpose
    pMAGIC L1-R5 entry plasmid, contains empty mouse U6-driven xCas9(3.7) (i.e. SpCas9) gRNA scaffold for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Type
    Empty backbone
    Use
    Synthetic Biology; Pmagic gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121816-plasmid-map-sequence-id-237364

    Enlarge

Showing: 10481 - 10500 of 26211 results