Addgene

We narrowed to 17,065 results for: gateway

Showing: 10481 - 10500 of 17065 results

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pPB-CAG-Tfe3-pgk-hph

Plasmid

#48755

Purpose

PiggyBac backbone, CAG promoter driving murine Tfe3, Hygromycin selection

Depositor

Austin Smith

Article

Betschinger et al Cell. 2013 Apr 11;153(2):335-47. doi: 10

Insert

Tfe3 (Tcfe3 Mouse)

Use

Piggybac

Tags

Expression

Mutation

Promoter

CAG

Available since

July 19, 2016

Availability

Academic Institutions and Nonprofits only
pCAGGS_2pabPAC

Plasmid

#158968

Purpose

2P-activatable bPAC for transient expression

Depositor

Michiyuki Matsuda

Article

Kinjo et al ACS Chem Biol. 2020 Oct 19. doi: 10.1021

Insert

2pabPAC

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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907H1

Plasmid

#160234

Purpose

FlightN-CymR-VP16-3X-SV40-attB

Depositor

Omar Akbari

Article

Gamez et al Insect Mol Biol. 2021 Oct;30(5):461-471.

Inserts

p-cymene repressor
Flightin promoter

Use

Tags

VP16

Expression

Insect

Mutation

Promoter

Available since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pKEB31

Plasmid

#31224

Depositor

Adam Bogdanove, Daniel Voytas

Article

Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

Insert

ccdB and Cm resistance gene

Use

Tags

Expression

Mutation

Promoter

Available since

June 30, 2011

Availability

Academic Institutions and Nonprofits only
pEF-ENTR C (709-1)

Plasmid

#17429

Purpose

Gateway entry vector with EF1-a promoter, V5-His C-term, frame C

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Entry vector

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pSN007

Plasmid

#102440

Purpose

For PCR amplification to create paired gRNAs to clone into Golden Gate gRNA expression vectors (e.g. pSB700 Plasmid #64046)

Depositor

Alejandro Chavez, George Church

Article

Nageshwaran et al J Vis Exp. 2018 Oct 2;(140). doi: 10.379

Insert

7SK paired gRNA insert

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pQCXIZ CMV/TO DEST (w321-2)

Plasmid

#17401

Purpose

Retroviral Gateway destination vector, Expression, CMV/TO promoter, Zeo

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Retroviral; Destination vector

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pMOS010E: Entry vector for: CheRiff-eGFP channelrhodopsin (plasma membrane)

Plasmid

#163063

Purpose

Entry vector for: CheRiff-eGFP channelrhodopsin (plasma membrane)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

CheRiff

Use

Gateway entry vector

Tags

eGFP

Expression

Mutation

Promoter

Available since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pDEST47-SAR1

Plasmid

#67472

Purpose

Mammalian expression of hSar1 with stop codon (native expression)

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

SAR1 (SAR1A Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDEST17-Nanog

Plasmid

#13988

Depositor

Shinya Yamanaka

Article

Mitsui et al Cell. 2003 May 30. 113(5):631-42.

Insert

Nanog (Nanog Mouse)

Use

Tags

His

Expression

Bacterial

Mutation

None

Promoter

Available since

March 16, 2007

Availability

Academic Institutions and Nonprofits only
NHA-TNGW1

Plasmid

#36437

Depositor

Edward Chan

Article

Yao et al Nucleic Acids Res. 2011 Apr;39(7):2534-4

Insert

TNRC6A (TNRC6A Human)

Use

Tags

NHA

Expression

Mammalian

Mutation

Full length (aa1-1962)

Promoter

Available since

June 1, 2012

Availability

Academic Institutions and Nonprofits only
bactin:GFP-DCX

Plasmid

#105961

Purpose

transgenesis, ubiquitous expression

Depositor

Caren Norden

Article

Icha et al J Cell Biol. 2016 Oct 24;215(2):259-275.

Insert

DCX (DCX Human)

Use

Tags

GFP

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pWS1037

Plasmid

#123049

Purpose

G_ subunit

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

Gpa1 [3-4a]

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

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pDEST51-LRRK2-Y1699C

Plasmid

#25084

Depositor

Mark Cookson

Article

J Biol Chem. 2008 Jun 13;283(24):16906-1

Insert

LRRK2 (LRRK2 Human)

Use

Destination vector

Tags

6XHis and V5

Expression

Mammalian

Mutation

Tyrosine 1699 mutated to Cysteine

Promoter

Available since

July 2, 2010

Availability

Academic Institutions and Nonprofits only
pSCM167.2-Venus-VF-hADAM10(aa1-213)

Plasmid

#189554

Purpose

Protein expression ADAM10 in mammalian cells. Contains only prodomain--amino acids 1-213

Depositor

Haian Fu

Insert

truncated ADAM metallopeptidase domain 10 (ADAM10 Human)

Use

Tags

VF

Expression

Mammalian

Mutation

contains aa 1-213

Promoter

Available since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pSCM167.2-Venus-VF-hADAM10(aa455-550)

Plasmid

#189556

Purpose

Protein expression ADAM10 in mammalian cells. Contains only disintegrin domain--amino acids 455-550

Depositor

Haian Fu

Insert

truncated ADAM metallopeptidase domain 10 (ADAM10 Human)

Use

Tags

VF

Expression

Mammalian

Mutation

contains aa 455-550

Promoter

Available since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pSCM167-Venus-VF-hADAM10(aa220-654)

Plasmid

#189560

Purpose

Protein expression ADAM10 in mammalian cells. Contains metalloprotease, disintegrin, and cysteine rich domains--amino acids 220-654

Depositor

Haian Fu

Insert

truncated ADAM metallopeptidase domain 10 (ADAM10 Human)

Use

Tags

VF

Expression

Mammalian

Mutation

contains aa 220-654

Promoter

Available since

Feb. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pSCM167-Venus-VF-hADAM10(aa220-459)

Plasmid

#189555

Purpose

Protein expression ADAM10 in mammalian cells. Contains only metalloprotease domain--amino acids220-459

Depositor

Haian Fu

Insert

truncated ADAM metallopeptidase domain 10 (ADAM10 Human)

Use

Tags

VF

Expression

Mammalian

Mutation

contains aa 220-459

Promoter

Available since

Nov. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pSCM167.2-Venus-VF-hADAM10(aa551-654)

Plasmid

#189557

Purpose

Protein expression ADAM10 in mammalian cells. Contains only cysteine rich domain--amino acids 551-654

Depositor

Haian Fu

Insert

truncated ADAM metallopeptidase domain 10 (ADAM10 Human)

Use

Tags

VF

Expression

Mammalian

Mutation

contains aa551-654

Promoter

Available since

Nov. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2-V5-DEST-hADAM10(aa1-213)-GST

Plasmid

#189562

Purpose

Protein expression ADAM10 in mammalian cells. Contains only prodomain--amino acids 1-213

Depositor

Haian Fu

Insert

truncated ADAM metallopeptidase domain 10 (ADAM10 Human)

Use

Tags

GST

Expression

Mammalian

Mutation

contains aa 1-213

Promoter

Available since

Nov. 11, 2022

Availability

Academic Institutions and Nonprofits only

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