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Plasmid#181918PurposeLentiviral plasmid that directs the expression of mutant NFE2L1/Nrf1 where 8 putative N-glycosites are mutated from asparagine to aspartic acid in mammalian cells.DepositorInsertNFE2L1 (NFE2L1 Human)
UseLentiviralTagsFLAG and HAExpressionMammalianMutation8 putative N-glycoyslation sites converted from a…PromoterSFFVAvailable sinceApril 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMSCV neo WT Akt1
Plasmid#14549DepositorInsertAkt1 (Akt1 Mouse)
UseRetroviralTagsHA tagExpressionMammalianMutationPromoterAvailable sinceApril 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
pHM106Env
Plasmid#139024PurposeExpress HIV Env which is isolated a from IRB approved HIV infected patient samples from Singapore and cloned into expression vector (pcDNA 3.1)DepositorInsertHIV-1 Envelope Glycoprotein (env HIV-1)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
NC3 pCDNA3.1 FLAG WTX (1-804)
Plasmid#36958DepositorInsertWTX (AMER1 Human)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceJuly 24, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLKO-1 shRev-Erbalpha
Plasmid#22747DepositorInsertshRev-Erbalpha (Nr1d1 Mouse)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 8, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCAG-hPPP3CB-FLAG
Plasmid#179135PurposeExpression vector of human protein phosphatase 3, catalytic subunit, beta isoform (PPP3CB) tagged with FLAG at C-terminus, CAG promoter, rabbit globin poly(A) signal.DepositorInsertprotein phosphatase 3, catalytic subunit, beta isoform (PPP3CB Human)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceJan. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRVdGL-4Flpo
Plasmid#98040PurposeSecond-generation rabies viral vector genomeDepositorInsertFlpo
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9(BB)-2A-Neo_h53BP1_gRNA_D
Plasmid#110300PurposeExpresssion of Cas9-T2A-neomycin resistant gene and a gRNA targeting exon 10 of human 53BP1DepositorInsertp53-binding protein 1 (TP53BP1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
NSP6-Flag
Plasmid#210345PurposeExpresses SARS-CoV-2 NSP6 fused with Flag in mammalian cellsDepositorInsertSARS-CoV-2 NSP6 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1, Human)
UseTagsFlagExpressionMammalianMutationMany synonymous changes due to codon optimizationPromoterAvailable sinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
NSP4-WT-Flag
Plasmid#210339PurposeExpresses SARS-CoV-2 NSP4 fused with Flag in mammalian cellsDepositorInsertSARS-CoV-2 NSP4 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1, Human)
UseTagsFlagExpressionMammalianMutationMany synonymous changes due to codon optimizationPromoterAvailable sinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLentiDP1.0
Plasmid#171094Purposelentiviral construct (3rd generation) for the expression of cell cycle-independent OsTIR1 fused with mEmerald and miniAID-fused mCherry2DepositorInsertOsTIR1-mEmerald-P2A-miniAID-mCherry2
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Rab7a-7
Plasmid#56443PurposeLocalization: Ras GTPase, Excitation: 488, Emission: 507DepositorInsertRab7a (RAB7A Human)
UseTagsEGFPExpressionMammalianMutationN117S in NM_004637.5PromoterCMVAvailable sinceJan. 13, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBABE-HA-GOLPH3
Plasmid#21687DepositorInsertGolgi Phosphoprotein 3 (GOLPH3 Human)
UseRetroviralTagsHAExpressionMammalianMutationPromoterAvailable sinceJuly 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pAAV_CAG_FLEX_PKIαmut-P2A-mRuby3_WPRE
Plasmid#196678PurposeAAV plasmid for mammalian Cre-dependent expression of PKIαmut-P2A-mRuby3 under a CAG promotor w/ WPRE enhancer. PKIαmut bears the R20G/R21G mutation that renders it non-functional.DepositorInsertPKIαmut-P2A-mRuby3
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-45b-RUNX2(WT)-IDR-mCherry
Plasmid#145286PurposeBacterial Expression of 6xHis RUNX2(WT) IDR mCherry Fusion ProteinDepositorInsertRUNX2(WT)-IDR (RUNX2 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRVdGL-4Cre
Plasmid#98039PurposeSecond-generation rabies viral vector genomeDepositorInsertmCre
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCR-II-TOPO-mitfa_LexPR-2A-Cerulean-SV40pA-FRT-Kan-FRT: Sequences,
Plasmid#110201PurposeMelanocyte-specific expression of the LexPR-2A-Cerulean fusionDepositorInsertLexPR transactivator - 2A - Cerulean
UseTagsCeruleanExpressionBacterialMutationPromoterAvailable sinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
LITE2.0 EF1a_TALE(Neurog2)-GS-CIB1(NLS*,∆318-334)_WPRE_hGHpA
Plasmid#47458PurposeLITE2.0 TALE-CIB1. Changes from LITE1.0: glycine-serine linker, mutated endogenous NLS, aa318-334 deletion. This particular TALE targeted to Neurog2. EF1-alpha promoter for ubiquitous mammalian exp.DepositorInsertTALE (Neurog2)-GS-CIB1(NLS*,∆318-334)
UseTagsHAExpressionMammalianMutationPromoterEF1-alphaAvailable sinceOct. 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
AAV-RO1.7-Lrat
Plasmid#206345PurposeAAV plasmid expressing LRAT in photoreceptorsDepositorInsertLrat (Lrat Mouse)
UseAAVTagsExpressionMutationPromoter1.7kb human red opsinAvailable sinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
tet-pLKO shMKP1.v1 puro
Plasmid#87790PurposeExpresses an inducible short hairpin targeting human MKP1 sequenceDepositorInsertshMKP1 (DUSP1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterTRE tightAvailable sinceMarch 27, 2017AvailabilityAcademic Institutions and Nonprofits only