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Addgene
Showing: 10401 - 10420 of 20580 results

  1. HCP5

    Plasmid
    #166107
    Purpose
    This plasmid encodes a Cas9 protein as well as a sgRNAs that targets the C-terminus of Cyr1.
    Depositor
    Matthias Heinemann
    Insert
    Cyr1CT-sgRNA (CYR1 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    166107-plasmid-map-sequence-id-330320

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  2. pHTganA-Cpf1

    Plasmid
    #158647
    Purpose
    Constitutive transcription of FnCpf1 and ganA crRNA
    Depositor
    Wenjing Cui
    Insert
    Cpf1, ganA crRNA
    Use
    CRISPR and Synthetic Biology; Shuttle vector baci…
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    P43
    Availability
    Academic Institutions and Nonprofits only
    158647-plasmid-map-sequence-id-314144

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  3. pADsacA

    Plasmid
    #158649
    Purpose
    Constitutive transcription of sacA crRNA and GFP
    Depositor
    Wenjing Cui
    Insert
    sacA crRNA
    Use
    CRISPR and Synthetic Biology; Shuttle vector baci…
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Pveg
    Availability
    Academic Institutions and Nonprofits only
    158649-plasmid-map-sequence-id-310569

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  4. pBsacA

    Plasmid
    #158650
    Purpose
    Constitutive transcription of sacA crRNA
    Depositor
    Wenjing Cui
    Insert
    sacA crRNA
    Use
    CRISPR and Synthetic Biology; Shuttle vector baci…
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Pveg
    Availability
    Academic Institutions and Nonprofits only
    158650-plasmid-map-sequence-id-314145

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  5. pRDA_221

    Plasmid
    #169142
    Purpose
    Constitutive expression of EGFP and sgRNAs targeted to EGFP for measurement of activity of AsCas12a\EnAsCas12a.
    Depositor
    David Root, John Doench
    Insert
    EGFP , EGFP sgRNA
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    169142-plasmid-map-sequence-id-334957

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  6. pTniQ-Cascade

    Plasmid
    #199659
    Purpose
    type I-F Cascade over-expression
    Depositor
    IIya Finkelstein
    Insert
    TniQ cas8 cas7 cas6
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    199659-plasmid-map-sequence-id-401151

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  7. mPLD3-sgRNA-Cas9-mcherry

    Plasmid
    #199700
    Purpose
    encodes sgRNA for mouse PLD3 KO, (target 217-223aa) plus Cas9-P2A-mCherry
    Depositor
    David Nemazee
    Insert
    hSpCas9
    Use
    CRISPR
    Tags
    P2A-RFP
    Expression
    Mammalian
    Mutation
    Promoter
    U6 promoter
    Availability
    Academic Institutions and Nonprofits only
    199700-plasmid-map-sequence-id-390617

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  8. hPLD3-sgRNA-Cas9_mcherry

    Plasmid
    #199342
    Purpose
    encodes sgRNA for human PLD3 KO, (target Exon 7) plus Cas9-P2A-mCherry
    Depositor
    David Nemazee
    Insert
    hSpCas9
    Use
    CRISPR
    Tags
    P2A-RFP
    Expression
    Mammalian
    Mutation
    Promoter
    human U6
    Availability
    Academic Institutions and Nonprofits only
    199342-plasmid-map-sequence-id-390619

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  9. hPLD4-sgRNA-Cas9_mcherry

    Plasmid
    #199343
    Purpose
    encodes sgRNA for human PLD4 KO, (target Exon 5) plus Cas9-P2A-mCherry
    Depositor
    David Nemazee
    Insert
    hSpCas9
    Use
    CRISPR
    Tags
    P2A-mCherry
    Expression
    Mammalian
    Mutation
    sgRNA: accagtagtatgaagccacg
    Promoter
    human U6
    Availability
    Academic Institutions and Nonprofits only
    199343-plasmid-map-sequence-id-390620

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  10. pCMV-ABE8e-NRCH

    Plasmid
    #165416
    Purpose
    Expresses the base editor ABE8e-NRCH in mammalian cells
    Depositor
    David Liu
    Insert
    ABE8e-NRCH
    Use
    Tags
    BP-NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    165416-plasmid-map-sequence-id-334022

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  11. pRha-ABE8e-NRCH

    Plasmid
    #165417
    Purpose
    For bacterial purification of ABE8e-NRCH protein
    Depositor
    David Liu
    Insert
    ABE8e-NRCH
    Use
    Tags
    8xHIS and BP-NLS
    Expression
    Bacterial
    Mutation
    Promoter
    pRhaBAD
    Availability
    Academic Institutions and Nonprofits only
    165417-plasmid-map-sequence-id-334014

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  12. pHACK-GAL4>GAL80

    Plasmid
    #104874
    Purpose
    HACK Donor plasmids for converting GAL4 lines to GAL80 lines using HACK method.
    Depositor
    Christopher Potter
    Insert
    T2A-GAL80
    Use
    CRISPR
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    104874-plasmid-map-sequence-id-204699

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  13. XLone-PE2

    Plasmid
    #136463
    Purpose
    Expresses prime editing 2 (PE2) enzyme upon doxycycline treatment
    Depositor
    Xiaojun Lian
    Insert
    PE2
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE3GS
    Availability
    Academic Institutions and Nonprofits only
    136463-plasmid-map-sequence-id-272540

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  14. TLCV2-LoxP

    Plasmid
    #127098
    Purpose
    LentiCRISPR v2 was modified into an all-in-one dox inducible system.
    Depositor
    Adam Karpf
    Insert
    Cas9-2A-eGFP
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    U6
    Availability
    Academic Institutions and Nonprofits only
    127098-plasmid-map-sequence-id-246495

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  15. TLCV2 sgAAVS1

    Plasmid
    #127099
    Purpose
    Encodes gRNA for human AAVS1.
    Depositor
    Adam Karpf
    Insert
    AAVS1 (PPP1R12C AAVS1 Human) (PPP1R12C Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    U6
    Availability
    Academic Institutions and Nonprofits only
    127099-plasmid-map-sequence-id-246497

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  16. pSpCas9(BB)-2A-Hygro

    Plasmid
    #127763
    Purpose
    Used for expression of sgRNA in mammalian cells
    Depositor
    Ken-Ichi Takemaru
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    127763-plasmid-map-sequence-id-247560

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  17. C41m

    Plasmid
    #120974
    Purpose
    MoClo golden gate assembly CD part for dCas9 (Catalytically-inactive S. pyogenes Cas9, for gRNA-targeted repression). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    dCas9
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    120974-plasmid-map-sequence-id-236028

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  18. T7m

    Plasmid
    #121030
    Purpose
    MoClo golden gate assembly DE part for tracrRNA terminator (natural terminator from S. pyogenes CRISPR-system tracrRNA). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    S. pyogenes tracrRNA terminator
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    121030-plasmid-map-sequence-id-236182

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  19. pLenti-ABE7.10-P2A-Puro

    Plasmid
    #112674
    Purpose
    Lentivirus for constitutive expression of ABE7.10 (not codon optimized)
    Depositor
    Lukas Dow
    Insert
    ABE7.10
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    D10A
    Promoter
    EF1s
    Availability
    Academic Institutions and Nonprofits only
    112674-plasmid-map-sequence-id-215665

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  20. pLinker-6xHA-HXGPRT-LoxP

    Plasmid
    #86552
    Purpose
    The plasmid is used for endogenous tagging using CRISPR technology in Toxoplasma
    Depositor
    David Sibley
    Insert
    Linker-6xHA-HGPRT-LoxP
    Use
    Expression in toxoplasma gondii
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    86552-plasmid-map-sequence-id-190138

    Enlarge

Showing: 10401 - 10420 of 20580 results