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Plasmid#46238PurposeFor expression of HA and FLAG-tagged murine CAD in mammalian cellsDepositorInsertcarbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (Cad Mouse)
UseTagsFLAG and HAExpressionMammalianMutationPromoterCMVAvailable sinceNov. 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSF3-Flag-Cbir-Ago2_Myc-Nbir-GFP
Plasmid#90015PurposeSplit-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-Ago2 and N-terminally tagged NBir[2-256]-GFPDepositorInsertsCBirA*-Ago2
NBirA*-GFP
UseRetroviral; Flp/frtTagsFLAG and MycExpressionMammalianMutationPromoterCMVAvailable sinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-NUP188
Plasmid#159893PurposeExpresses NUP188 in mammalian cellsDepositorInsertNUP188 (NUP188 Human)
UseTagsEGFPExpressionMammalianMutationTo avoid NotI site, first three amino acids are d…PromoterCMVAvailable sinceOct. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PE2-tagRFP-BleoR
Plasmid#192508PurposeExpression of PE2 in mammalian cells with co-expression of tagRFP and Zeocin resistance gene.DepositorInsertPE2-P2A-tagRFP-P2A-BleoR
UseTagsBleoR, SV40 bpNLS, and tagRFPExpressionMammalianMutationPromoterAvailable sinceJan. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ΔN-RNMT
Plasmid#112708PurposeExpresses FLAG-NES-RNMT 121-476 D203A ("ΔN-RNMT") in mammalian cells. Overexpression inhibits cytoplasmic cap methylation.DepositorInsertRNMT (RNMT Human)
UseTagsFLAG and HIV Rev nuclear export signal (NES)ExpressionMammalianMutationdeleted amino acids 2-120, mutated active-site D2…PromoterCMVAvailable sinceAug. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMJA285= pSin-TCRab-CMVp-Puro
Plasmid#116875Purposestable TCR expression in human T cellsDepositorUseLentiviralTagsExpressionMammalianMutationdeletion of EF1-a promotorPromoterCMV PromotorAvailable sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
CSAC-Pers
Plasmid#168281PurposeExpresses sgRNA in mammalian cellsDepositorInserthU6-sgPer1-3-hU6-sgPer2-1-hU6-sgPer2-2
UseAAVTagsmCherryExpressionMutationPromoterU6, hSynAvailable sinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSF3-Flag-Cbir-Ago2_Myc-Nbir-TNRC6C
Plasmid#90014PurposeSplit-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-Ago2 and N-terminally tagged NBir[2-256]-TNRC6CDepositorInsertsCBirA*-Ago2
NBirA*-TNRC6C
UseRetroviral; Flp/frtTagsFLAG and MycExpressionMammalianMutationPromoterCMVAvailable sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAX-EGFP-FBP17
Plasmid#178365PurposeExpresses FBP17 c-terminal labeled with EGFP in mammalian cellsDepositorInsertFormin Binding Protein 17 (FNBP1 Human)
UseTagsExpressionMammalianMutationPromoterChicken Beta ActinAvailable sinceMarch 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSF3-Flag-Cbir-Cdc25C WT_Myc-Nbir-GFP
Plasmid#90012PurposeSplit-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-Cdc25C and N-terminally tagged NBir[2-256]-GFPDepositorInsertsCBirA*-Cdc25C
NBirA*-GFP
UseRetroviral; Flp/frtTagsFLAG and MycExpressionMammalianMutationPromoterCMVAvailable sinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSF3-Flag-Cbir-Ago2_Myc-Nbir-Dicer
Plasmid#90013PurposeSplit-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-Ago2 and N-terminally tagged NBir[2-256]-DicerDepositorInsertsCBirA*-Ago2
NBirA*-Dicer
UseRetroviral; Flp/frtTagsFLAG and MycExpressionMammalianMutationPromoterCMVAvailable sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-DYRK2-2A-GFP
Plasmid#118284PurposeExpresses mouse DYRK2 tagged with 2A peptide at it's C-terminus and GFP in mammalian cells.DepositorInsertdual-specificity tyrosine-(Y)-phosphorylation regulated kinase 2 (Dyrk2 Mouse)
UseAAVTags2A peptide and GFPExpressionMammalianMutationPromoterCMV with beta global intronAvailable sinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBC2T-Ypet-FtnA
Plasmid#108969Purposeexpresses BC2-tag-Ypet-FtnA in mammalian cellsDepositorInsertFtnA (ftnA Escherichia coli)
UseTagsBC2-tag and YpetExpressionMammalianMutationPromoterAvailable sinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-VIM-BC2T
Plasmid#108966Purposeexpresses mCherry-vimentin-BC2-tag in mammalian cellsDepositorInsertVIM (VIM Human)
UseTagsBC2T and mCherryExpressionMammalianMutationPromoterAvailable sinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-PDGFRalpha-Y206S
Plasmid#136435PurposeMammalian expression plasmid for c-myc-tagged PDGFRA (Y206S mutant)DepositorInsertPDGFRalpha (PDGFRA Human)
UseTagsExtracellular c-myc epitope tag and Signal peptid…ExpressionMammalianMutationY206S (disrupts PDGF binding, but still binds HCM…PromoterCMVAvailable sinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-PDGFRalpha-V242K
Plasmid#136436PurposeMammalian expression plasmid for c-myc-tagged PDGFRA (V242K mutant)DepositorInsertPDGFRalpha (PDGFRA Human)
UseTagsExtracellular c-myc epitope tag and Signal peptid…ExpressionMammalianMutationV242K (disrupts PDGF binding, but still binds HCM…PromoterCMVAvailable sinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
Alt AlphaPS3
Plasmid#14070DepositorInsertDrosphila Alt Alpha PS3 Integrin (scb Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
FP5369 KIF17MD-flag- FIREmate
Plasmid#216724PurposeExpresses motor domain of KIF17 fused to FIREmate in mamallian cellsDepositorInsertKIF17 (KIF17 Human)
UseTagsFIREmate and FlagExpressionMammalianMutation1-547PromoterCMVAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-IFT43
Plasmid#218735PurposeExpresses N-terminally EGFP-tagged IFT43 in mammalian cellsDepositorInsertIFT43 (IFT43 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -