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Plasmid#89247PurposeExpresses GFP with +9 theoretical charge in E.ColiDepositorInsertHis6-(pos9)-GFP
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceApril 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLX304-VHL-IRES-GFP
Plasmid#187641PurposeVHL Expression vector with GFPDepositorInsertVHL (VHL Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBrain-GFP-TACC3KDP-shTACC3
Plasmid#59356PurposeKnocks down endogenous TACC3 and re-expresses knockdown-proof (RNAi-resistant) GFP-tagged human TACC3.DepositorInsertTransforming Acidic Coiled Coil protein 3 (TACC3 Human)
UseRNAiTagsGFPExpressionMammalianMutationSilent mutations in amino acids 30-33 to confer s…PromoterCMVAvailable sinceDec. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-EGFP-Rac1(T17N)
Plasmid#13721DepositorInsertEGFP-Rac1(T17N) (RAC1 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceMarch 28, 2007AvailabilityAcademic Institutions and Nonprofits only -
TetOn-REEP5-mCherry-eGFP
Plasmid#139870PurposeDox-inducible lentiviral expression of human REEP5DepositorInsertREEP5 (REEP5 Human)
UseLentiviralTagsmCherry-eGFPExpressionMutationPromoterAvailable sinceMarch 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
GFP-pcDNA3-PKCgamma-cys1Acys1B
Plasmid#21190DepositorInsertPKCgamma-cys1Acys1B (Prkcg Rat)
UseTagsGFPExpressionMammalianMutationPKCgamma-cys1Acys1B (aa35 to aa152 of rat PKCgamm…PromoterAvailable sinceJuly 15, 2009AvailabilityAcademic Institutions and Nonprofits only -
MSCV h c-MYC IRES GFP
Plasmid#18119DepositorInsertc-MYC (MYC Human)
UseRetroviralTagsGFPExpressionMutationThe CUG which is the start site for Myc I is pres…PromoterAvailable sinceJune 9, 2008AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Puro Xlone-eGFP
Plasmid#136936Purposedonor plasmid for inducible transgene expression in human cellsDepositorInsertall-in-one tet-on system
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterTRE3GSAvailable sinceApril 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-GFP-EphrinB3-Flag
Plasmid#155012PurposeExpresses N-terminally GFP-tagged and C-terminally Flag-tagged EphrinB3 from pcDNA3.1DepositorInsertEphrinB3 (EPHB3 Human)
UseTagsFlag, GFP, and signal peptide (residues 1 to 32) …ExpressionMammalianMutationPromoterCMVAvailable sinceNov. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCS2-SP-FRB (T2098L) -sfGFP-TM-V5
Plasmid#186227PurposemRNA synthesis of rapalog-induced cell adhesion moleculeDepositorInsertmRNA synthesis of rapalog-induced cell adhesion molecule
UseTagsV5ExpressionMammalianMutationFRB (T2098L)PromoterAvailable sinceJuly 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
GFP-Rab35 S22N inactive
Plasmid#47426DepositorInsertRab35 S22N (RAB35 Human)
UseTagsGFPExpressionMammalianMutationS22N inactivePromoterCMVAvailable sinceJuly 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV hSyn GFP-Cre
Plasmid#175381Purposeexpresses GFP-Cre under the control of a human synapsin promoterDepositorInsertGFP-Cre recombinase
UseAAVTagsCre is fused to GFP. GFP is fused to additional n…ExpressionMutationPromoterhuman synapsinAvailable sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
Str-KDEL_SBP-EGFP-Ecadherin
Plasmid#65286Purposesynchronize trafficking of Ecadherin from the ER (RUSH system)DepositorInsertEcadherin
UseTagsEGFP and IL-2 Signal Sequence and Streptavidin Bi…ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
PGK-IKZF3-CTERM-GFP
Plasmid#185779PurposeDegron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloningDepositorInsertGFP
UseLentiviralTagsV5, IKZF3 minidegronExpressionMutationnonePromoterPGKAvailable sinceSept. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-SRGAP1FBAR(516a.a.)-EGFP
Plasmid#168505PurposeExpression of F-BAR domain (aa1-516) of SRGAP1 with a C-terminal EGFP fusion.DepositorInsertSRGAP1-FBAR (SRGAP1 Human)
UseTagsEGFPExpressionMammalianMutationaa 1-516PromoterCAGAvailable sinceJune 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
p667-UBC-GFP-V5-TurboID_IDG-K
Plasmid#135241PurposeGateway destination clone of GFP (as control) tagged with C-terminal V5-TurboID for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertGFP-V5-TurboID
UseLentiviral; Gateway destinationTagsV5-TurboIDExpressionMammalianMutationPromoterUbiquitinAvailable sinceJan. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET19b-EcMscL-TEV-mEGFP
Plasmid#165097PurposeExpresses EcMscLGFP as a fusion protein in E. coli BL21or using in vitro expression.DepositorInsertE. Coli Mechanosensitive Channel of Large Conductance (mscL E. coli)
UseSynthetic BiologyTagsmonomeric Enhanced Green Fluorescent ProteinExpressionBacterialMutationPromoterT7Available sinceFeb. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pegfp-N1-TFEB-S3A,R4A
Plasmid#44446DepositorInsertTFEB (TFEB Human)
UseTagsGFPExpressionMammalianMutationChanged both Serine 3 and Arginine 4 to AlaninePromoterCMVAvailable sinceApril 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMXs-puro GFP-DFCP1
Plasmid#38269DepositorInsertZinc finger FYVE domain-containing protein 1 (Zfyve1 Mouse)
UseRetroviralTagsEGFPExpressionMammalianMutationPromoterAvailable sinceAug. 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pQE-60NA-proinsulin-mEGFP
Plasmid#160464PurposeEncodes a fusion of human proinsulin and the monomeric enhanced EGFP. This construct can be used as a negative control for superfolder activity in bacteriaDepositorInsertproinsulin-mEGFP
UseTagsExpressionBacterialMutationPromoterT5Available sinceMarch 8, 2021AvailabilityAcademic Institutions and Nonprofits only