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pLKO-RFP-IKZF3-sh3
Plasmid#69044Purpose3rd generation lentiviral vector expressing IKZF3 shRNADepositorInsertIKZF3 (IKZF3 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterU6Available sinceOct. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
GFP-TRIM66
Plasmid#65400PurposeGFP-tagged BRD protein, which can be used to be expressed in mammalian cells.DepositorInsertTRIM66 (TRIM66 Human)
UseTagsGFP and V5ExpressionMammalianMutationnonePromoterCMVAvailable sinceAug. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28C-mCherry-HNRNPA1LCD
Plasmid#204405PurposeE. coli expression of HNRNPA1 low-complexity domain (186-320) with an N-terminal mCherry tagDepositorInsertHNRNPA1 (186-320) (HNRNPA1 Human)
UseTags6x-His; mCherryExpressionBacterialMutationPromoterT7Available sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSUPER-hHSF1 shRNA
Plasmid#118345PurposeThis plasmid expresses shRNA against hHSF1.DepositorInserthHSF1 shRNA (HSF1 Human)
UseTagsExpressionMammalianMutationWTPromoterAvailable sinceNov. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcsII-pb2-flag
Plasmid#86766PurposeC-terminal flag-tagged protein expression in mammalian cells, lentiviral vectorDepositorInsertproteasome beta subunit 2 (PSMB2 Human)
UseLentiviralTagsflagExpressionMammalianMutationPromoterEF-1aAvailable sinceJan. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pTSara
Plasmid#60720PurposeExpresses both fragments of T7 RNAP split at position 179. Both fragments are driven by the arabinose inducible promoter PBAD. Also contains a constitutive araC ORFDepositorInsertsN Terminal fragment of T7 RNAP split betwen 179-180
N Terminal fragment of T7 RNAP split betwen 179-180
UseTagsExpressionBacterialMutationT7 RNAP split between positions 179-180 and wt T7…PromoterPBADAvailable sinceDec. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-BG505.DS-SOSIP.4mut-gp160
Plasmid#123277PurposeMammalian expression plasmid for Env from the BG505 HIV-1 isolate; DS-SOSIP.4mut variantDepositorInsertHIV-1 (BG505) Env
UseTagsCD5 leader peptideExpressionMammalianMutationCodon-optimized synthetic gene; SOSIP mutations; …PromoterCMVAvailable sinceApril 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-BG505.SOSIP-gp160-754*
Plasmid#123274PurposeMammalian expression plasmid for Env from the BG505 HIV-1 isolate (containing SOSIP mutations); C-terminal truncationDepositorInsertHIV-1 (BG505) Env
UseTagsCD5 leader peptideExpressionMammalianMutationCodon-optimized synthetic gene; SOSIP mutations; …PromoterCMVAvailable sinceApril 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLKO-RFP-IKZF3-sh2
Plasmid#69043Purpose3rd generation lentiviral vector expressing IKZF3 shRNADepositorInsertIKZF3 (IKZF3 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterU6Available sinceOct. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSH231-EF1-BLST-dCas9-VPR
Plasmid#115148PurposeSafe harbor site 231 knock-in vector with BlastR-dCas9-VPR expression cassetteDepositorInsertBlastR-P2A-dCas9-VPR
UseCRISPRTagsNucleoplasmin NLS and SV40NLSExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mCherry-TSC2(AA)-NLS
Plasmid#192443PurposeEncodes mCherry-tagged TSC2 isoform 5 with S939A, T1439A mutations to block Akt phosphorylation; targeted to the nucleus.DepositorInsertmCherry-TSC2(S939A,T1439A)-NLS (TSC2 Human)
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationTSC2 Ser 939 and Thr 1439 (equivalent to 1462 in …PromoterCMVAvailable sinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGL3-Basic-hCYP26A1-FL- luciferase
Plasmid#135566PurposeFull Length promoter of the human CYP26A1 gene in pGL3-Basic-luciferaseDepositorInsertFull-length promoter of human retinoic acid receptor-beta gene
UseTagsExpressionMutationPromoterhHuman CYP26A1 gene promoterAvailable sinceJan. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pLX317-SNAI1
Plasmid#115446PurposeConstitutive lentiviral expressionDepositorInsertSNAI1 (SNAI1 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceSept. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-GFP(+36)-DL4
Plasmid#199168Purposeused to express a supercharged GFP variant that forms inclusion bodies in E. coliDepositorInsertGFP(+36)-DL4
UseTagsMGHHHHHHGGAExpressionMutationE16R, T19R, V21K, D29K, E42K, E44K, N49E, D86K, Q…PromoterT7Available sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1-CIB1-Myc
Plasmid#32505DepositorInsertCIB1 (CIB1 Human)
UseTagsMycExpressionMammalianMutationPromoterAvailable sinceJan. 26, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-FLAG-GPD1
Plasmid#32487DepositorInsertGPD1 (Gpd1 Mouse)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 28, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-BG505.DS-SOSIP.6mut-gp160
Plasmid#123278PurposeMammalian expression plasmid for Env from the BG505 HIV-1 isolate; DS-SOSIP.6mut variantDepositorInsertHIV-1 (BG505) Env
UseTagsCD5 leader peptideExpressionMammalianMutationCodon-optimized synthetic gene; SOSIP mutations; …PromoterCMVAvailable sinceApril 3, 2019AvailabilityAcademic Institutions and Nonprofits only