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Plasmid#124714PurposeExpression of torS under Ptac and torT under PD59_luxRDepositorUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only
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QSOX1-luciferase
Plasmid#113352Purposeevaluate FOXA1 DIV motif enhancer activity near 50 kb of QSOX1 geneDepositorInsertQSOX1-enhancer (QSOX1 Human)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pAC4 Grb14-PIRSH2
Plasmid#46438DepositorInsertGrb14 (GRB14 Human)
UseTagsAviTag, GST, and Thrombin siteExpressionBacterialMutationcontains amino acids 341-540PromoterTrcAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
R725-X09-635: His6-tev-Hs.SPRED1(13-125)
Plasmid#159581PurposeBaculovirus protein expression of His6-tev-Hs.SPRED1(13-125)DepositorInsertHis6-tev-Hs.SPRED1(13-125) (SPRED1 Human)
UseTagsHis6-tevExpressionInsectMutationPromoterAvailable sinceSept. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV10/3xflag-Clock L57E/L113E/W284A
Plasmid#47343Purposetriple mutation used for co-IPDepositorInsertClock (Clock Mouse)
UseTags3x FlagExpressionMammalianMutationL57E/L113E/W284APromoterCMVAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
p.UTA.2.0 miR-27a-3p
Plasmid#82438PurposeDual Fluorescent endogenous miRNA sensor. Perfect complementary region of hsa-miR-27a-3pDepositorInserthsa-miR-27a-3p (MIR27A Human)
UseTagsExpressionMutationThree copies hsa-miR-27a-3p Complementary region …PromoterAvailable sinceMarch 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-Nsp2
Plasmid#157682Purposemammalian expression of untagged SARS-CoV-2 Nsp2 under control of a tetracycline-inducible promoterDepositorInsertNsp2 (ORF1ab SARS-CoV-2)
UseTagsExpressionMammalianMutationcodon optimizedPromoterAvailable sinceAug. 4, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEBTetBl-CLIP-Cep57
Plasmid#136866PurposeMammalian expression of the centrosomal protein Cep57 N-terminally fused to CLIP-tagDepositorInsertCLIP-Cep57 (CEP57 Synthetic, Human)
UseTagsFLAG-tag / His-tagExpressionMammalianMutationPromoterCMV-TetO2Available sinceMarch 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_CD2AP-2/3
Plasmid#91391PurposeProtein expression and purification of human SH3 domain construct CD2AP-2/3DepositorInsertCD2AP-2/3 (CD2AP Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEBTetBl-CLIP-Cep164
Plasmid#136873PurposeMammalian expression of the centrosomal protein Cep164 N-terminally fused to CLIP-tagDepositorInsertCLIP-Cep164 (CEP164 Synthetic, Human)
UseTagsFLAG-tag / His-tagExpressionMammalianMutationPromoterCMV-TetO2Available sinceMarch 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pVL48 - pAt2g38380>>
Plasmid#116014Purposedestination vector for GreenGate cloning method, contains 2x a set of modules from A-F, "Driver line", tissues-specific promoter, Dex-inducible mTurquoise2 expressionDepositorInsertpAT2G38380:GR-LhG4:tAt4g24550:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR
UseTagsHDEL and SP(ER)ExpressionPlantMutationPromoterAvailable sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVL58 - pAPL>>
Plasmid#116015Purposedestination vector for GreenGate cloning method, contains 2x a set of modules from A-F, "Driver line", tissues-specific promoter, Dex-inducible mTurquoise2 expressionDepositorInsertpAPL:GR-LhG4:tAPL:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR
UseTagsHDEL and SP(ER)ExpressionPlantMutationPromoterAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-Nsp2-HF
Plasmid#157684Purposemammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp2 under control of a tetracycline-inducible promoterDepositorInsertNsp2-HF (ORF1ab SARS-CoV-2)
UseTagsHis8-FlagExpressionMammalianMutationcodon optimizedPromoterAvailable sinceAug. 4, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
G protein alpha-o-R179C-EE
Plasmid#55780PurposeThis is a constitutively activated G protein alpha-o mutant containing an internal EE epitope.DepositorInsertG protein alpha-o with constitutively activating R179C mutation (Gnao1 Rat)
UseTagsEE epitope was introduced internally with D167E a…ExpressionBacterial and MammalianMutationArginine 179 was replaced with cysteinePromoterCMVAvailable sinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pmiRGLO-CD164-201
Plasmid#117329PurposeDual luciferase assay for miRNA-targeted UTRDepositorInsert3' UTR CD164-201
UseLuciferaseTagsluciferaseExpressionMammalianMutationPromoterMurine PGK-1Available sinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 (-) hYAP 1-2beta
Plasmid#124144PurposeProtein expression for funtional studies of cell growth promotionDepositorInsertYAP1 (YAP1 Human)
UseUnspecifiedTagsExpressionMutationNone (Wild Type)PromoterAvailable sinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 (-) hYAP 1-1gamma
Plasmid#124141PurposeProtein expression for funtional studies of cell growth promotionDepositorInsertYAP1 (YAP1 Human)
UseUnspecifiedTagsExpressionMutationNone (Wild Type)PromoterAvailable sinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-HA/Bmal I317D
Plasmid#47347PurposePcHA-Bmal backbone used for the above mutation. Ref.Genes & Dev 17:1921-1932 2003DepositorInsertBMAL (Bmal1 Mouse)
UseTagsHaExpressionMammalianMutationI317DPromoterCMVAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBScMXT + mSlo1 MBr5/23
Plasmid#16151DepositorInsertMus musculus potassium large conductance calcium-activated channel (Kcnma1 Mouse)
UseXenopus oocyte expressionTagsExpressionMutationN/APromoterAvailable sinceJan. 10, 2008AvailabilityAcademic Institutions and Nonprofits only