Addgene

We narrowed to 50,572 results for: plasmid

Showing: 1021 - 1040 of 50572 results

Results per page:

Sort results by:

pET22-Nsp10-TEV-6His

Plasmid

#154957

Purpose

Expresses Nsp10 from SARS-CoV-2 with a C-terminal TEV-protease cleavable 6His tag in E. coli

Depositor

Justin Kollman

Insert

Nsp10-TEV-6His (ORF1ab SARS-CoV-2)

Use

Tags

TEV-6His

Expression

Bacterial

Mutation

Promoter

T7 Promoter

Available since

July 21, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pBabe puro IkB alpha M

Plasmid

#12332

Depositor

Inder Verma

Insert

IkB alpha M (Nfkbia Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Dominant-negative mutant. Many signal transductio…

Promoter

Available since

Aug. 17, 2006

Availability

Academic Institutions and Nonprofits only
pELK3.1.0-gDNA

Plasmid

#112418

Purpose

CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ELK3

Depositor

Kevin White

Insert

ELK3 (ELK3 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
XLone-BSD SARS-CoV2 N P2A mCherry

Plasmid

#154398

Purpose

PiggybacTransposon-based tunable and temporal expression control of SARS-CoV2 N and mCherry

Depositor

Xiaoping Bao

Insert

SARS-CoV2 N Protein (N SARS-CoV2)

Use

Piggybac

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

TRE3G

Available since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
ANO10 (TMEM16KA-c001)

Plasmid

#110267

Purpose

Baculovirus expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

TMEM16K (ANO10 Human)

Use

Tags

TEV-His10-Flag

Expression

Insect

Mutation

Promoter

polyhedrin promoter

Available since

April 25, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-Ef1a-DIO-Synaptophysin-GCaMP6s

Plasmid

#105715

Purpose

In the presence of Cre, the plasmid can be used to express GCaMP6s fused to the presynaptic protein synaptophysin. Drives enriched expression of GCaMP6s in neuronal axon boutons.

Depositor

Rylan Larsen

Has Service

AAV5

Insert

synaptophysin-GCaMP6s

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

Ef1a, Human elongation factor-1 alpha

Available since

April 13, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pJUNB.1.0-gDNA

Plasmid

#112409

Purpose

CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor JUNB

Depositor

Kevin White

Insert

JUNB (JUNB Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pATF4.1.0-gDNA

Plasmid

#113772

Purpose

CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ATF4

Depositor

Kevin White

Insert

ATF4 (ATF4 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pZD-CJ1111

Plasmid

#175058

Purpose

rbcLXS6803 integrative plasmid expresses CRE recombinase as a translational fusion to rbcL6803

Depositor

David Nielsen

Article

Jones et al ACS Synth Biol. 2021 Sep 17;10(9):2371-2

Insert

CRE

Use

Unspecified

Tags

Expression

Mutation

Promoter

rbcLXS

Available since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28-Nsp14-TEV-6His

Plasmid

#154952

Purpose

Expresses Nsp14 from SARS-CoV-2 with a C-terminal TEV-protease cleavable 6His tag in E. coli

Depositor

Justin Kollman

Insert

Nsp14-TEV-6His (ORF1ab SARS-CoV-2)

Use

Tags

TEV-6His

Expression

Bacterial

Mutation

Promoter

T7 Promoter

Available since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
JMJD1CA-c089

Plasmid

#79644

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

JMJD1C (JMJD1C Human)

Use

Tags

6x His and TEV cleavage site

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 16, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pICE ADE2

Plasmid

#122876

Purpose

ADE2 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

Depositor

Randy Hampton

Article

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 25, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
ACE2 g2

Plasmid

#153012

Purpose

A guide RNA targeting ACE2 in a lentiviral plasmid co-expressing mCherry

Depositor

Jason Sheltzer

Insert

ACE2 gRNA (ACE2 Human)

Use

CRISPR and Lentiviral

Tags

mCherry

Expression

Mutation

Promoter

Available since

June 1, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
ACE2 g3

Plasmid

#153013

Purpose

A guide RNA targeting ACE2 in a lentiviral plasmid co-expressing mCherry

Depositor

Jason Sheltzer

Insert

ACE2 (ACE2 Human)

Use

CRISPR and Lentiviral

Tags

mCherry

Expression

Mutation

Promoter

Available since

June 1, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pICE URA3

Plasmid

#122882

Purpose

URA3 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

Depositor

Randy Hampton

Article

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Tags

Expression

Yeast

Mutation

Promoter

Available since

Dec. 3, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCJ111

Plasmid

#175052

Purpose

rbcLXS7002 integrative plasmid expresses CRE recombinase as a translational fusion to rbcL

Depositor

David Nielsen

Article

Jones et al ACS Synth Biol. 2021 Sep 17;10(9):2371-2

Insert

CRE

Use

Unspecified

Tags

Expression

Mutation

Promoter

rbcLXS

Available since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28-6His-SUMO-Nsp14

Plasmid

#154956

Purpose

Expresses Nsp14 from SARS-CoV-2 with a N-terminal SUMO-protease cleavable 6His-SUMO tag in E. coli

Depositor

Justin Kollman

Insert

6His-SUMO-Nsp14 (ORF1ab SARS-CoV-2)

Use

Tags

6His-Thrombin-SUMO

Expression

Bacterial

Mutation

Promoter

T7 Promoter

Available since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
GALTA-c001

Plasmid

#79645

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

GALT (GALT Human)

Use

Tags

6x His and TEV cleavage site

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 16, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pICE HIS3

Plasmid

#122877

Purpose

HIS3 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

Depositor

Randy Hampton

Article

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pESR1.1.0-gDNA

Plasmid

#112433

Purpose

CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ESR1

Depositor

Kevin White

Insert

ESR1 (ESR1 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge