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Plasmid#179078PurposeSp.Cas9 and gRNA yeast expression vector for HDR-based gRNA introduction. Selection = Kanamycin resistanceDepositorInsertS. pyogenes Cas9
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pG3H-U6SC
Plasmid#134755PurposepGreen3 CRISPR/Cas9DepositorInsertCRISPR/Cas9
UseCRISPRTagsExpressionMutationPromotergRNA-U6, zCas9-35SCAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pQCi-mB2m-sgRNA
Plasmid#154090PurposepQCi backbone with sgRNA targeting murine Beta-2-microglobulinDepositorInsertsgRNA targeting murine B2M locus
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJuly 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pG3K-U6SC
Plasmid#134754PurposepGreen3 CRISPR/Cas9DepositorInsertCRISPR/Cas9
UseCRISPRTagsExpressionMutationPromotergRNA-U6, zCas9-35SCAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pG3B-U3Ub
Plasmid#134750PurposepGreen3 CRISPR/Cas9DepositorInsertCRISPR/Cas9
UseCRISPRTagsExpressionMutationPromotergRNA- OsU3, zCas9-Ubi1Available sinceDec. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pX459V2.0-SpCas9-HF4
Plasmid#108295PurposepX459 V2.0 (Plasmid #62988) with the Y450A, N497A, R661A, Q695A, and Q926A mutationsDepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMay 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pG3H-U6EC1
Plasmid#134758PurposepGreen3 CRISPR/Cas9DepositorInsertCRISPR/Cas9
UseCRISPRTagsExpressionMutationPromotergRNA-U6, zCas9-EC1Available sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pG3K-U6EC1
Plasmid#134757PurposepGreen3 CRISPR/Cas9DepositorInsertCRISPR/Cas9
UseCRISPRTagsExpressionMutationPromotergRNA-U6, zCas9-EC1Available sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pG3B-U6EC1
Plasmid#134756PurposepGreen3 CRISPR/Cas9DepositorInsertCRISPR/Cas9
UseCRISPRTagsExpressionMutationPromotergRNA-U6, zCas9-EC1Available sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pG3H-U3Ub
Plasmid#134749PurposepGreen3 CRISPR/Cas9DepositorInsertCRISPR/Cas9
UseCRISPRTagsExpressionMutationPromotergRNA- OsU3, zCas9-Ubi1Available sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pG3GB411
Plasmid#134748PurposepGreen3 CRISPR/Cas9DepositorInsertCRISPR/Cas9
UseCRISPRTagsExpressionMutationPromotergRNA- OsU3, zCas9-Ubi1Available sinceDec. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBBK02 pCAS-Tyr-[gRNA: BsaI GFP dropout] (SplitKanR, AmpR)
Plasmid#178986PurposeSp.Cas9 and gRNA yeast expression vector for Golden Gate pre-cloning of gRNA. Selection = SplitKanamycin resistanceDepositorInsertS. pyogenes Cas9
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK10 pCAS-Tyr-[gRNA: BsaI GFP dropout] (SplitURA3,AmpR)
Plasmid#178994PurposeSp.Cas9 and gRNA yeast expression vector for Golden Gate pre-cloning of gRNA. Selection = SplitURA3DepositorInsertS. pyogenes Cas9
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK09 pCAS-Tyr-[gRNA: BsaI GFP dropout] (BsmBI-flanked URA3, AmpR)
Plasmid#178993PurposeSp.Cas9 and gRNA yeast expression vector for HDR-based gRNA introduction. Selection = URA3DepositorInsertS. pyogenes Cas9
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK08 pCAS-Tyr-[gRNA: BsaI GFP dropout] (SplitLEU2,AmpR)
Plasmid#178992PurposeSp.Cas9 and gRNA yeast expression vector for Golden Gate pre-cloning of gRNA. Selection = SplitLEU2DepositorInsertS. pyogenes Cas9
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK07 pCAS-Tyr-[gRNA: BsaI GFP dropout] (LEU2,AmpR)
Plasmid#178991PurposeSp.Cas9 and gRNA yeast expression vector for HDR-based gRNA introduction. Selection = LEU2DepositorInsertS. pyogenes Cas9
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK06 pCAS-Tyr-[gRNA: BsaI GFP dropout] (SplitNatR,AmpR)
Plasmid#178990PurposeSp.Cas9 and gRNA yeast expression vector for Golden Gate pre-cloning of gRNA. Selection = SplitNourseothrycin resistanceDepositorInsertS. pyogenes Cas9
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX459V2.0-eSpCas9(1.1)+K855A
Plasmid#108298PurposepX459 V2.0 (Plasmid #62988) with the K848A, K855A, K1003A, and R1060A mutationsDepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMay 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pX459V2.0-eSpCas9(1.1)+K810A
Plasmid#108297PurposepX459 V2.0 (Plasmid #62988) with the K810A, K848A, K1003A, and R1060A mutationsDepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMay 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCas9
Plasmid#42876PurposeBacterial expression of Cas9 nuclease, tracrRNA and crRNA guideDepositorInserttracr/Cas9
UseCRISPR; E.coliTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 26, 2013AvailabilityAcademic Institutions and Nonprofits only