We narrowed to 9,881 results for: aav

1. pGP-AAV-syn-FLEX-jGCaMP7b-WPRE

   Plasmid

   #104493

   Purpose

   AAV-mediated expression of ultrasensitive protein calcium sensor under the Syn promoter, Cre mediated flip-excision switch

   Depositor

   Douglas Kim, GENIE Project

   Article

   Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

   Has Service

   AAV Retrograde and AAV1

   Insert

   jGCaMP7b
   

   Use

   AAV and Cre/Lox

   Tags

   T7 epitope, Xpress tag, 6xHis

   Expression

   Mammalian

   Promoter

   Synapsin

   Available Since

   April 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGP-AAV-CAG-FLEX-jGCaMP7b-WPRE

   Plasmid

   #104497

   Purpose

   AAV-mediated expression of ultrasensitive protein calcium sensor under the CAG promoter, Cre mediated flip-excision switch

   Depositor

   Douglas Kim, GENIE Project

   Article

   Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

   Insert

   jGCaMP7b
   

   Use

   AAV and Cre/Lox

   Tags

   T7 epitope, Xpress tag, 6xHis

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Feb. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAV-CMV-LOXP-stop-LOXP-mG-CaMP3.0

   Plasmid

   #50022

   Purpose

   expresses Membrane tagged G-CaMP3 in cells expressing Cre

   Depositor

   David Anderson

   Article

   Vrontou et al Nature. 2013 Jan 31;493(7434):669-73. do

   Insert

   G-CaMP3
   

   Use

   AAV

   Tags

   MARCKS sequence (MGCCFSKT)

   Promoter

   CMV

   Available Since

   June 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-camk2-Sthk-p2a-bpac(WT) minWPRE

   Plasmid

   #118274

   Purpose

   Expresses the SthK channel & bPAC(WT)-mCherry under a minimal CamKII promoter

   Depositor

   Dietmar Schmitz

   Article

   Bernal Sierra et al Nat Commun. 2018 Nov 5;9(1):4611. doi: 1

   Inserts

   bPAC(WT)
   SthK
   

   Use

   AAV

   Tags

   His tag, P2A, mCherry, and myc tag

   Expression

   Mammalian

   Promoter

   CamK II

   Available Since

   Jan. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAV-syn-ChR2-Kv-P2A-H2b-mRuby2

   Plasmid

   #89570

   Purpose

   Channelrhodopsin localized to neuronal soma with simultaneous nuclear labeling

   Depositor

   McLean Bolton

   Article

   Baker et al Elife. 2016 Aug 15;5. pii: e14193. doi:

   Inserts

   Channelrhodopsin
   Histone 2B
   

   Use

   AAV

   Tags

   mRuby2

   Expression

   Mammalian

   Mutation

   H134R

   Promoter

   synapsin

   Available Since

   Feb. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-RSV-GFP-U6-gRNA scaffold (SpyCas9)

   Plasmid

   #113039

   Purpose

   AAV vector; encodes GFP as well as a U6-driven gRNA scaffold (SpyCas9)

   Depositor

   Dominik Niopek

   Article

   Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

   Insert

   2x BbsI sites - SpCas9 scaffold, co-expressed GFP (transfection marker)
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   Nov. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. aav-SYN-tdNfsB(F124W)-mCherry (eNTR)

   Plasmid

   #113760

   Purpose

   AAV-mediated expression of bacterial tdNfsB-mCherry (eNTR) under the SYN promoter

   Depositor

   Luke Lavis

   Article

   Gruber et al ACS Chem Biol. 2018 Aug 29. doi: 10.1021

   Insert

   tdNfsB(F124W)-mCherry
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   F124W

   Promoter

   Synapsin

   Available Since

   Oct. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pOTTC1012 - pAAV EF1a DIO Nuc-eYFP

   Plasmid

   #75082

   Purpose

   An AAV packaging vector that expresses Cre-dependent nuclear-localized eYFP under control of the EF1a promoter.

   Depositor

   Brandon Harvey

   Article

   Sharpe et al Curr Biol. 2017 Jul 24;27(14):2089-2100.

   Insert

   Nuc-eYFP
   

   Use

   AAV and Cre/Lox

   Tags

   NLS

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Jan. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJEP309-pAAV-EFS-dSaCas9-KRAB-Dio-pA

   Plasmid

   #113686

   Purpose

   An EFS driven inverted de-catalyzed SaCas9 fused to the transcription repressor KRAB. dSaCas9-KRAB is floxed to render the system cre-dependent.

   Depositor

   Jonathan Ploski

   Article

   Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

   Insert

   de-catalyzed SaCas9
   

   Use

   AAV, CRISPR, and Cre/Lox

   Tags

   KRAB

   Available Since

   Feb. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJEP308-pAAV-EFS-dSaCas9-VP64-Dio-pA

    Plasmid

    #113685

    Purpose

    An EFS driven inverted de-catalyzed SaCas9 fused to the transcription activator VP64. dSaCas9-VP64 is floxed to render the system cre-dependent.

    Depositor

    Jonathan Ploski

    Article

    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

    Insert

    inverted de-catalyzed SaCas9
    

    Use

    AAV, CRISPR, Cre/Lox, and Synthetic Biology

    Tags

    NLS and VP64

    Available Since

    Dec. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-CaMKII-Archon1-KGC-EGFP-ER2

    Plasmid

    #108419

    Purpose

    AAV production plasmid encoding for Archon1 fluorescent voltage reporter

    Depositor

    Edward Boyden

    Article

    Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

    Insert

    Archon1-KGC-EGFP-ER2
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CaMKII

    Available Since

    April 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV_hsyn_NES-his-CaMPARI2-H396K-WPRE-SV40

    Plasmid

    #101062

    Purpose

    AAV vector expressing CaMPARI2_H396K (Kd = 360nM), a photoconvertible fluorescent protein-based calcium integrator, in neurons

    Depositor

    Eric Schreiter

    Article

    Moeyaert et al Nat Commun. 2018 Oct 25;9(1):4440. doi:

    Insert

    CaMPARI2-H396K
    

    Use

    AAV

    Tags

    FLAG-HA-myc and NES_his

    Promoter

    hsyn (synapsin-1)

    Available Since

    Oct. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. YA1627: pAAV_hSyn-Dio-paQuasAr3-s-P2A-CheRiff-s

    Plasmid

    #107704

    Purpose

    in vivo voltage imaging

    Depositor

    Adam Cohen

    Article

    Adam et al Nature. 2019 May 1. pii: 10.1038/s41586-

    Insert

    paQuasAr3-P2A-CheRiff
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    May 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV Syn hBE Rh Guanylyl Cyclase1 2A tDimer

    Plasmid

    #66779

    Purpose

    The rhodopsin-guanylyl cyclase of the aquatic fungus Blastocladiella emersonii enables fast optical control of cGMP signaling

    Depositor

    Thomas Oertner

    Article

    Scheib et al Sci Signal. 2015 Aug 11;8(389):rs8. doi:

    Inserts

    hbeRhGC
    red fluorescent protein
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    human Synapsin

    Available Since

    Oct. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV CAG ChR2 E123T T159C 2A tDimer

    Plasmid

    #85399

    Purpose

    High efficiency channelrhodopsin with cytomegalovirus enhancer fused to chicken β-actin (CAG) promoter

    Depositor

    Thomas Oertner

    Article

    Berndt et al Proc Natl Acad Sci U S A. 2011 May 3;108

    Inserts

    Channelrhodopsin-2
    red fluorescent protein
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    E123T , T159C

    Promoter

    CAG and ribosomal skip sequence 2A

    Available Since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. rAAV-CAG::FLEX-rev:ChR2HA:2a:PSAML141F,Y115F:GlyR

    Plasmid

    #32483

    Purpose

    Cre-dependent expression of ChR2 and PSAM-GlyR (L141F,Y115F) neuronal inhibitor, with IRES-EGFP marker

    Depositor

    Scott Sternson

    Article

    Magnus et al Science. 2011 Sep 2;333(6047):1292-6.

    Insert

    ChR2HA-2a-PSAML141F,Y115F:GlyR
    

    Use

    AAV and Cre/Lox; Adeno-associated virus, flex swi…

    Tags

    ChR2 2A

    Promoter

    CAG

    Available Since

    April 11, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-Ef1a-DIO hChR2(E123A)-mCherry

    Plasmid

    #35508

    Purpose

    AAV expression of EF1a-driven, cre-dependent, hChR2 variant CheTA 2.0 for ultrafast optogenetic control.

    Depositor

    Karl Deisseroth

    Article

    Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

    Insert

    hChR2
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    E123A

    Promoter

    Ef1a

    Available Since

    April 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-CMV-FRT-myrSNAP-2A-H2BeGFP-WPRE

    Plasmid

    #102986

    Purpose

    AAV expressing membrane-targeted SNAP protein and nuclear-targeted eGFP after FLP-mediated recombination

    Depositor

    Marco Tripodi

    Article

    Ciabatti et al Cell. 2017 Jul 1. pii: S0092-8674(17)306

    Inserts

    membrane-targeted SNAP protein after FLP-mediated recombination
    Nuclear-targeted eGFP after FLP-mediated recombination
    

    Use

    AAV; Adeno associated viral vector

    Available Since

    Dec. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-synP-FLEX-TVA66T-EGFP-B19G

    Plasmid

    #64097

    Purpose

    Expresses TVA66T-EGFP-B19G under the synapsin promotor

    Depositor

    Ian Wickersham

    Insert

    TVA66T-EGFP-B19G
    

    Use

    AAV and Cre/Lox

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Glutamic acid 66 to Threonine

    Promoter

    synapsin

    Available Since

    April 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV Syn ChR E90R-T159C 2A tDimer

    Plasmid

    #52495

    Purpose

    Historical plasmid. For inhibition of neuronal activity, note that Addgene Plasmid #66709 is a light-gated chloride channel with improved anion selectivity (iChloC)

    Depositor

    Thomas Oertner

    Article

    Wietek et al Science. 2014 Mar 27.

    Inserts

    Channelrhodopsin-2
    red fluorescent protein
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    E90R, T159C

    Promoter

    human synapsin-1 and ribosomal skip sequence

    Available Since

    April 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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