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We narrowed to 4,146 results for: Pet2;

Showing: 1021 - 1040 of 4146 results

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VV001: WT Arch-3 in pET28b

Plasmid

#58487

Purpose

Expresses WT Archaerhodopsin-3 (with 6x C-terminal His tag) in E. coli

Depositor

Adam Cohen

Article

Maclaurin et al Proc Natl Acad Sci U S A. 2013 Apr 9;110

Insert

Archaerhodopsin-3 (with C-terminal His tag)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 23, 2014

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1-E988K

Plasmid

#173945

Purpose

Bacterial expression of inactive PARP1 mutant (E988K attenuates catalytic and PAR-branching activity of PARP1)

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-E988K (PARP1 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

V762A, E988K

Promoter

Available since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1-L713F

Plasmid

#174794

Purpose

Bacterial expression of a hyperactive PARP1 mutant (L713F destabilizes the autoinhibitory HD subdomain)

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-L713F (PARP1 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

L713F, V762A

Promoter

Available since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1-C125G

Plasmid

#174792

Purpose

Bacterial expression of a less active PARP1 mutant (C125G reduces affinity for single-strand DNA break)

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-C125G (PARP1 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

C125G, V762A

Promoter

Available since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a-IMX-DogTag

Plasmid

#113765

Purpose

Expresses IMX-DogTag in bacterial cells for modular antigen multimerization using SnoopLigase

Depositor

Mark Howarth

Article

Andersson et al Sci Rep. 2019 Mar 15;9(1):4625. doi: 10.

Insert

IMX-DogTag

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 18, 2019

Availability

Academic Institutions and Nonprofits only

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pET22b-GFP-CPDSalI

Plasmid

#38257

Depositor

Matthew Bogyo, Aimee Shen

Article

Shen et al PLoS One. 2009 Dec 2;4(12):e8119.

Insert

eGFP

Use

Tags

6xHis and Vibrio cholerae MARTX toxin cysteine pr…

Expression

Bacterial

Mutation

cloned from pEGFPN3 (Clonetech)

Promoter

T7

Available since

July 20, 2012

Availability

Academic Institutions and Nonprofits only
pET21TtTERT(195-516)

Plasmid

#28172

Depositor

Kathleen Collins

Article

O'Connor et al J Biol Chem. 2005 Apr 29. 280(17):17533-

Insert

Tetrahymena TERT

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 24, 2011

Availability

Academic Institutions and Nonprofits only
pET28-iLOV-Rsn2

Plasmid

#61687

Purpose

bacterial expression of ranaspumin 2 as a fusion protein with the fluorescent tag iLOV

Depositor

Brian Smith

Insert

Ranaspumin-2

Use

Tags

His and iLOV

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 2, 2015

Availability

Academic Institutions and Nonprofits only

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pET28b-SpRY-His

Plasmid

#179320

Purpose

Plasmid for bacterial expression and purification of SpRY-Cas9

Depositor

Julián Cerón, Miguel Angel Moreno-Mateos

Article

Vicencio et al Nat Commun. 2022 May 12;13(1):2601. doi:

Insert

Human codon-optimized Cas9 variant SpRY

Use

CRISPR

Tags

6xHis and SV40-NLS

Expression

Bacterial

Mutation

SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/ N13…

Promoter

Available since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1-CATdeltaHD

Plasmid

#174811

Purpose

Bacterial expression of a hyperactive PARP1 CAT lacking the autoinhibitory HD subdomain

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-CATdeltaHD (PARP1 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Residues 678-787 replaced by eight-residue linker…

Promoter

Available since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pET29b-Ava3858-3856

Plasmid

#45745

Depositor

Christopher T Walsh, Emily Balskus

Article

Balskus et al Science. 2010 Sep 24;329(5999):1653-6. d

Insert

Ava3858-3856

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 8, 2013

Availability

Academic Institutions and Nonprofits only
pET28a-mAmetrine-CNA35

Plasmid

#61604

Purpose

bacterial expression of N-terminal fusion protein of CNA35 with mAmetrine

Depositor

Maarten Merkx

Article

Aper et al PLoS One. 2014 Dec 9;9(12):e114983. doi:

Insert

mAmetrine and CNA35

Use

Tags

His-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 23, 2015

Availability

Academic Institutions and Nonprofits only

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pET21-Streptavidin-K121R

Plasmid

#89880

Purpose

Streptavidin that has unimpaired biotin binding after dye labeling

Depositor

Mark Howarth

Article

Jacobsen et al Cell Chem Biol. 2017 Jul 17. pii: S2451-

Insert

Streptavidin-K121R-Glutamate_Tag

Use

Tags

6 glutamate tag (C terminal on insert)

Expression

Bacterial

Mutation

K121R

Promoter

Available since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pET28b-ETV6-ETS

Plasmid

#111180

Purpose

Minimal ETS domain of human ETV6 (TEL)

Depositor

Gregory Poon

Article

Vo et al J Biol Chem. 2017 Aug 11;292(32):13187-1

Insert

ETV6 ETS domain (ETV6 Bovine, Human, Mouse)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG-41

Plasmid

#172757

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-41; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-41

Use

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pET28b-miniE-DBCO

Plasmid

#181985

Purpose

Used to label proteins with the MiniR genetically encoded tag

Depositor

Kimberly Beatty

Article

Doh et al Biochemistry. 2020 Aug 25;59(33):3051-30

Insert

MiniE-DBCO

Use

Tags

DBCO-Tag, His-Tag

Expression

Bacterial

Mutation

Promoter

Available since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

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pET28b-miniR-DBCO

Plasmid

#181984

Purpose

Used to label proteins with the MiniE genetically encoded tag

Depositor

Kimberly Beatty

Article

Doh et al Biochemistry. 2020 Aug 25;59(33):3051-30

Insert

MiniR-DBCO

Use

Tags

DBCO-Tag, His-Tag

Expression

Bacterial

Mutation

Promoter

Available since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

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pET28-sfGFP[N150TAA]

Plasmid

#164581

Purpose

superfolder GFP with N150TAA mutation

Depositor

Ryan Mehl

Article

Bednar et al ACS Chem Biol. 2021 Nov 19;16(11):2612-2

Insert

sfGFP-150TAA

Use

Tags

6xHis

Expression

Bacterial

Mutation

N150TAA Mutation

Promoter

T7

Available since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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RanBP2-RB3-4-pET23a

Plasmid

#85769

Purpose

bacterial expression of RanBP2 SUMO E3 ligase fragment (aa 2304-3062) with C-terminal His tag

Depositor

Frauke Melchior

Article

Werner et al Mol Cell. 2012 May 11;46(3):287-98. doi:

Insert

RanBP2 fragment (RANBP2 Human)

Use

Tags

His

Expression

Bacterial

Mutation

fragment (amino acids 2304-3062)

Promoter

T7

Available since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pET28a-pro-sumo2

Plasmid

#25102

Depositor

Guy Salvesen

Article

Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-2

Insert

pro-SUMO2 (SUMO2 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

June 24, 2010

Availability

Academic Institutions and Nonprofits only