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Plasmid#124432PurposeExpresses FlipGFP (caspase-3 cleavage sequence) protease reporter in zebrafish (verified)DepositorInsertFlipGFP(Casp3 cleavage seq)
UseZebrafish (good expression)TagsExpressionMutationPromoterAvailable sinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUSE-Src-YF-UniRapR-mCerulean-myc
Plasmid#45381DepositorInsertSrc-YF-UniRapR-mCerulean-myc
UseTagsMyc, Myr, and mCeruleanExpressionMammalianMutationchanged mSrc Tyrosine 529 to PhenylalaninePromoterCMVAvailable sinceNov. 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
PhOTO-N (pMTB:memb-Cerulean-2A-H2B-Dendra2)
Plasmid#92400PurposeConstitutive expression of nuclear green-to-red photoconvertible Dendra2 and the membrane blue fluorescent protein Cerulean.DepositorInsertsDendra2
Cerulean
UseZebrafish expressionTagsH2B tag and membrane tagExpressionMutationPromoterAvailable sinceJuly 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
paavCAG-JxOFF-pre-mGRASP-mCerulean
Plasmid#51900PurposeJxOFF-preDepositorInsertpresynaptic mGRASP
UseAAV and Cre/LoxTagsExpressionMutationPromoterAvailable sinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBRY-nuclear mCherry-IRES-PURO
Plasmid#52409PurposeConstitutively expressed mammalian expression vector encoding nuclear mCherry fluorescent reporter. Puromycin resistance.DepositorInsertnuclear mCherry
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCS2plus-mCerulean 156-239-GGGS
Plasmid#162616PurposeAllows for transcription of control mCerulean fluorophore half for injection into zebrafish embryos for BiFC assaysDepositorInsertmCerulean
UseTagsExpressionMammalianMutation156-239PromoterAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVC-Ds-ubb:MCP-p65-HSF1-2a-Cerulean-Ds
Plasmid#119074PurposeZebrafish ubiquitin promoter driving MCP-p65-HSF1 with Cerulean reporterDepositorInsertubb:MCP-p65-HSF1-2a-Cerulean
UseTagsExpressionBacterialMutationPromoterZebrafish ubiquitin promoterAvailable sinceFeb. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 mCerulean LIC cloning vector (6E)
Plasmid#30128DepositorTypeEmpty backboneUseTagsmCeruleanExpressionMammalianMutationPromoterAvailable sinceJune 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
PhOTO-M (pMTB:memb-Dendra2-2A-H2B-Cerulean)
Plasmid#92401PurposeConstitutive expression of membrane green-to-red photoconvertible Dendra2 and the nuclear blue fluorescent protein Cerulean.DepositorInsertsDendra2
Cerulean
UseZebrafish expressionTagsH2B tag and membrane tagExpressionMutationPromoterbactin2Available sinceJuly 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUSE-Src-KD-UniRapR-mCerulean-myc
Plasmid#45382DepositorInsertSrc-KD-UniRapR-mCerulean-myc
UseTagsmCerulean and mycExpressionMammalianMutationmSrc R388D (kinase dead), Y529FPromoterCMVAvailable sinceAug. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMT-ubb-Avi-Cerulean-Rpl10a
Plasmid#79882PurposeUbiquitin promoter driving Avi-tagged protein containing Cerulean protein fused to zebrafish Rpl10a ribosomal unit (Tryon et al. 2012); flanked by Tol2 sequencesDepositorInsertCerulean protein fused to zebrafish Rpl10a ribosomal unit (rpl10a Zebrafish)
UseTagsAviExpressionBacterialMutationPromoterUbiquitinAvailable sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-Pur-mCerulean3-cpVenus[E172]
Plasmid#125203PurposeSB-transposon plasmid for stable expression of mCerulean3-cpVenus[E172] FRET high controlDepositorInsertmCerulean3-cpVenus[E172]
UseTransposonTagsExpressionMammalianMutationPromoterEF1a/RPBSAAvailable sinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBabe-TetCMV-puro-mCerulean-PA-Rac1
Plasmid#22031DepositorInsertmCerulean-PA-Rac1 (RAC1 Avena sativa (oat), Human)
UseRetroviralTagsExpressionMammalianMutationRac1 starts at I4 and contains mutations Q61L, E9…PromoterAvailable sinceSept. 29, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCDH-CAG-IntCCCre-EF1a-Cerulean
Plasmid#160508PurposeExpresses IntCCCre in mammalian cells in split intein-mediated split-Cre recombinase applicationsDepositorInsertIntCCCre
UseCre/Lox and LentiviralTagsmCeruleanExpressionMammalianMutationPromoterCAGAvailable sinceJan. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMT-ubb-Avi-Cerulean-RanGAP
Plasmid#79881PurposeUbiquitin promoter driving Avi-tagged protein containing Cerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1); flanked by Tol2 sequencesDepositorInsertCerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1) (RANGAP1 Chicken)
UseTagsAviExpressionBacterialMutationPromoterUbiquitin promoterAvailable sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pXL-CAG-ICeuI-Cerulean-2A-sHRPbNLG1
Plasmid#79912PurposepiggyBac transposon vector, Cerulean-2A-sHRPbNLG1DepositorInsertCerulean-2A-sHRPbNLG1
UsePiggybacTagsExpressionMammalianMutationPromoterAvailable sinceAug. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
Tol2 Krt4 Src (YF)-uniRapR-cerulean
Plasmid#45509DepositorInsertSrc (YF)-uniRapR-cerulean
UseZebrafish expressionTagsceruleanExpressionMutationY732FPromoterkrt4Available sinceOct. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
PhOTO-C (pMTB:cyto-Dendra2-2A-H2B-Cerulean)
Plasmid#92402PurposeConstitutive expression of cytosolic green-to-red photoconvertible Dendra2 and the nuclear blue fluorescent protein Cerulean.DepositorInsertsDendra2
Cerulean
UseZebrafish expressionTagsH2B tag and No tag, cytosolic expressionExpressionMutationPromoterbactin2Available sinceJuly 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
Cerulean(159-238) in pcDNAI/Amp
Plasmid#60898PurposeThis is a vector that attaches Cerulean(159-238) to the N-terminus of a protein.DepositorInsertCerulean(159-238)
UseTagsExpressionMammalianMutationContains N164H substitutionPromoterCMVAvailable sinceJuly 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MUC2 D1 with cleavable His tag
Plasmid#181813PurposeExpresses human MUC2 D1 with cleavable His tag, residues 21-389DepositorInsertMuc2 D1 (MUC2 Human)
UseTags6xHistadine followed by TEV cleavage siteExpressionMammalianMutationPromoterCMVAvailable sinceJan. 4, 2023AvailabilityAcademic Institutions and Nonprofits only