Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Addgene
Showing: 1021 - 1040 of 1204 results

  1. pCAG-denAsCas12a(E174R/S542R/K548R/D908A)-NLS(nuc)-3xHA-VPR (RTW776)

    Plasmid
    #107943
    Purpose
    Mammalian expression plasmid for human codon optimized DNase-inactive enAsCas12a (enhanced AsCas12a) fused to the VPR activation domain, encoding E174R/S542R/K548R/D908A substitutions
    Depositor
    Benjamin Kleinstiver, Keith Joung
    Article
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized DNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to VPR activation domain
    Use
    Tags
    3x HA, NLS (nucleoplasmin), and VPR (VP64-p65-Rta…
    Expression
    Mammalian
    Mutation
    E174R, S542R, K548R and D908A
    Promoter
    CAG
    Availability
    Academic Institutions and Nonprofits only
    107943-plasmid-map-sequence-id-207614

    Enlarge

  4. pDZ033

    Plasmid
    #159353
    Purpose
    Expresses rhlI and sfCFP (both tagged with ssrA) from the Pcin promoter.
    Depositor
    Matthew Bennett
    Insert
    rhlI
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Pcin
    Availability
    Academic Institutions and Nonprofits only
    159353-plasmid-map-sequence-id-315064

    Enlarge

  20. pGAL4-DBD-HOXB1-AroLITE_A

    Plasmid
    #215590
    Purpose
    For overexpression of Gal4-DBD HOXB1 AroLITE A
    Depositor
    Denes Hnisz
    Insert
    HOXB1 AroLITE A (HOXB1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    215590-plasmid-map-sequence-id-426933

    Enlarge

Showing: 1021 - 1040 of 1204 results