-
Plasmid#158953PurposeExpress human RAD51(T309A) mutant in mammalian cellsDepositorInsertRAD51 (RAD51 Human)
UseTags2X FLAG 2X STREPExpressionMammalianMutationchanged threonine 309 to alaninePromoterCMVAvailable sinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
-
pRetroX GFP Mps1 wt
Plasmid#63702PurposeInducible expression of GFP-Mps1 fusion in mammalian cellsDepositorInsertMps1 (TTK Human)
UseRetroviralTagsEGFPExpressionMammalianMutationPromotertight TRE promoterAvailable sinceApril 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
CiDr-VSP L223F-mCherry pcDNA3.1 (eVSP)
Plasmid#140892PurposeExpresses modified Danio rerio VSP without phosphatase activity (inactive mutant of enhanced VSP) fused with mCherry in mammalian cells.DepositorUseTagsmCherryExpressionMammalianMutationchanged 223L to F in Dr-VSP (zebrafish TPTE)PromoterAvailable sinceApril 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCT-CMV-UBE3B-copGFP-Puro
Plasmid#107342PurposeExpresses UBE3B fused to copGFP in mammalian cells.DepositorInsertUbiquitin-protein ligase E3B (UBE3B Human)
UseLentiviralTagscopGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 19, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
AAV.CBA.YFP.miR-E_shControl
Plasmid#180386PurposeProducing AAV that encodes negative control shRNA with miR-E backboneDepositorInsertshControl
UseAAV and RNAiTagsExpressionMutationPromoterCBAAvailable sinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pETM33_CLTC_Clathrin propeller
Plasmid#178471PurposeBacterial expression of human domain with His-tag and GST tagDepositorInsertCLTC_Clathrin propeller (CLTC Synthetic, Human)
UseTagsHis-GSTExpressionBacterialMutationPromoterT7/LacOAvailable sinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
plenti-UbcP-FLAG-CRBN-pGK-HYG
Plasmid#107374PurposeLentiviral expression of FLAG-CRBNDepositorInsertcereblon (CRBN Human)
UseTagsFLAGExpressionMammalianMutationPromoterUbcPAvailable sinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA6-N-3XFLAG-Vcp
Plasmid#123600DepositorInsertvalosin containing protein (Vcp Mouse)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1alpha-nCoV2019-N-meGFP-IRES-Puro
Plasmid#185451PurposeExpresses SARS-CoV-2 nucleocapsid protein with a c-terminal monomeric EGFP in mammalian cellsDepositorInsertNucleocapsid (N SARS-CoV-2)
UseLentiviralTagsmEGFPExpressionMammalianMutationPromoterEF1alphaAvailable sinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-shRNA
Plasmid#82697PurposeFor targeting shRNA construct into human AAVS1 locus, using genome editing. Expresses shRNA of interest (cloning: EcoRI and AgeI) under U6 promoter, flanked by AAVS1 homology arms.DepositorTypeEmpty backboneUseRNAiTagsExpressionMutationPromoterhPGK, U6Available sinceNov. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pX462-hPRKAA2-gRNA_A
Plasmid#74376PurposegRNA_A to knockout human AMPK alpha 2 using Cas9n.DepositorInserthuman AMPK alpha 2 exon1 gRNA (PRKAA2 Human)
UseCRISPRTagsExpressionMutationPromoterU6Available sinceApril 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
p7SL-neo-TET
Plasmid#51285Purposeexpresses human 7SL RNA, using its own polIII enhancer, and tagged with the self splicing intron neo cassetteDepositorInsert7SL RNA (RN7SL2 Human)
UseTagsneoTET cassette with a tetrahymena self-splicing …ExpressionMammalianMutationPromoter7SL upstream pollII enhancerAvailable sinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTP261
Plasmid#104166PurposeBacterial expression plasmid of anti-GFP nanobody with 1x Cysteine for maleimide labelingDepositorInsertAnti-GFP nanobody Enhancer PDB 3K1K (1x Cysteine)
UseTags14x Histidine tag and NEDD8 from Brachypodium dis…ExpressionBacterialMutation1x Cysteine located at bps 493-495 in full sequen…PromoterAvailable sinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCT-CMV-UBE3B(C1036A)-copGFP-Puro
Plasmid#107344PurposeExpresses UBE3B mutant C1036A fused to copGFP in mammalian cells.DepositorInsertUBE3B(C1036A)copGFP (UBE3B Human)
UseLentiviralTagscopGFPExpressionMammalianMutationmutation C1036APromoterCMVAvailable sinceApril 19, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAV.CMV.SV40.THBS4-HA.SV40(polyA)
Plasmid#155193PurposeExpresses murine Thrombospondin-4 (THBS4) protein with HA-tag at C-terminusDepositorInsertThrombospondin-4 (Thbs4 Mouse)
UseAAVTagsHA-tagExpressionMammalianMutationPromoterCMVAvailable sinceAug. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
GFP-SP140
Plasmid#65394PurposeGFP-tagged BRD protein, which can be used to be expressed in mammalian cells.DepositorInsertSP140 (SP140 Human)
UseTagsEmGFP and V5ExpressionMammalianMutationnonePromoterCMVAvailable sinceMay 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pX462-hPRKAA2-gRNA_B
Plasmid#74377PurposegRNA_B to knockout human AMPK alpha 2 using Cas9nDepositorInserthuman AMPK alpha 2 exon1 gRNA (PRKAA2 Human)
UseCRISPRTagsExpressionMutationPromoterU6Available sinceApril 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV_CAG_FLEX_PKIα-P2A-mRuby3_WPRE
Plasmid#196677PurposeAAV plasmid for mammalian Cre-dependent expression of PKIα-P2A-mRuby3 under a CAG promotor w/ WPRE enhancer. PKIα (protein kinase inhibitor α) inhibits the activity of protein kinase A (PKA).DepositorInsertPKIα-P2A-mRuby3
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
kiCAP-AAV-M.Mus.2
Plasmid#196682PurposeRep/Cap plasmid for the production of M.Mus.2, an AAV capsid with CNS tropism in mice.DepositorInsertAAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
UseAAVTagsExpressionMutationREQQKLW insert between amino acids 588 and 589 of…Promoterp41Available sinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only