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Addgene
Showing: 10341 - 10360 of 20580 results
  1. pROS15_MATz

    Plasmid
    #166100
    Purpose
    Expression of dual gRNA targeting the MAT locus
    Depositor
    Insert
    MAT Z1
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  2. HCP9

    Plasmid
    #166111
    Purpose
    This plasmid encodes a Cas9 protein as well as a sgRNA that targets the C-terminus of Hta2
    Depositor
    Insert
    Hta2-sg18 (HTA2 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  3. pY114 (pcDNA3.1-huPgCpf1)

    Plasmid
    #92273
    Purpose
    Expression of humanized PgCpf1
    Depositor
    Insert
    huPgCpf1
    Use
    Tags
    NLS and NLS-3xHA
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  4. pY120 (pcDNA3.1-huFbCpf1)

    Plasmid
    #92296
    Purpose
    Expression of humanized FbCpf1
    Depositor
    Insert
    huFbCpf1
    Use
    Tags
    NLS and NLS-3xHA
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  5. pY122 (pcDNA3.1-huCPbCpf1)

    Plasmid
    #92298
    Purpose
    Expression of humanized CPbCpf1
    Depositor
    Insert
    huCPbCpf1
    Use
    Tags
    NLS and NLS-3xHA
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  6. pY123 (pcDNA3.1-huCMaCpf1)

    Plasmid
    #92299
    Purpose
    Expression of humanized CMaCpf1
    Depositor
    Insert
    huCMaCpf1
    Use
    Tags
    NLS and NLS-3xHA
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  7. pY126 (pcDNA3.1-huBoCpf1)

    Plasmid
    #92302
    Purpose
    Expression of humanized BoCpf1
    Depositor
    Insert
    huBoCpf1
    Use
    Tags
    NLS and NLS-3xHA
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  8. pCRII-BbsI-sgRNAscaffold

    Plasmid
    #159352
    Purpose
    Expression vector for a customizable guide RNA
    Depositor
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Availability
    Academic Institutions and Nonprofits only
  9. pR6K-GFP-RFP

    Plasmid
    #199652
    Purpose
    R6K backbone with Golden Gate (GG) cloning sites
    Depositor
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23119 promoter
    Availability
    Academic Institutions and Nonprofits only
  10. CROP-seq-opti-dsRed

    Plasmid
    #201999
    Purpose
    Based on CROP-seq-opti (addgene #106280), we replaced the PURO resistance gene with a dsRed.
    Depositor
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    dsRed
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Availability
    Academic Institutions and Nonprofits only
  11. pCMV-T7 RNA POL

    Plasmid
    #138352
    Purpose
    Expressing T7 RNA Polymerase in mammalian cells
    Depositor
    Insert
    T7 RNA POL
    Use
    CRISPR and Lentiviral
    Tags
    No
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  12. pLinker-2xTy-HXGPRT-LoxP

    Plasmid
    #86664
    Purpose
    CRISPR tagging with 2xTy in Toxoplamsa gondii
    Depositor
    Insert
    Linker-2xTy-HXGPRT 3'UTR
    Use
    Expression in toxoplasma gondii
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  13. BPK1520 sgFADD

    Plasmid
    #211527
    Purpose
    Deletes FADD
    Depositor
    Insert
    sgPIDD
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  14. pHR Ef1a-dCas9-BFP-KRAB

    Plasmid
    #217304
    Purpose
    lentiviral vector for dCas9-BFP-KRAB on Ef1alpha promoter
    Depositor
    Insert
    dCas9-tagBFP-KRAB
    Use
    CRISPR, Lentiviral, and Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Ef1alpha
    Availability
    Academic Institutions and Nonprofits only
Showing: 10341 - 10360 of 20580 results