Addgene

We narrowed to 14,773 results for: LEA

Showing: 10321 - 10340 of 14773 results

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mEos2-LaminA-C-18

Plasmid

#57394

Purpose

Localization: Nuclear Envelope, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Insert

LaminA (LMNA Human)

Use

Tags

mEos2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits
MZB215

Plasmid

#221767

Purpose

Bacterial expression of human Arf6 (Q67L) with C-terminal 'self-cleaving' CPD-10xHis tag

Depositor

Richard Baker

Insert

Arf6 (ARF6 Human)

Use

Tags

CPD-10xHis

Expression

Bacterial

Mutation

Q67L

Promoter

T7 Promoter

Available since

Jan. 21, 2025

Availability

Academic Institutions and Nonprofits only

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MZB218

Plasmid

#221769

Purpose

Bacterial expression of human Arf4 (Q71L) with C-terminal 'self-cleaving' CPD-10xHis tag

Depositor

Richard Baker

Insert

Arf4 (ARF4 Human)

Use

Tags

CPD-10xHis

Expression

Bacterial

Mutation

Q71L

Promoter

T7 Promoter

Available since

Jan. 21, 2025

Availability

Academic Institutions and Nonprofits only

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MZB219

Plasmid

#221770

Purpose

Bacterial expression of human Arf5 (Q71L) with C-terminal 'self-cleaving' CPD-10xHis tag

Depositor

Richard Baker

Insert

Arf5 (ARF5 Human)

Use

Tags

CPD-10xHis

Expression

Bacterial

Mutation

Q71L

Promoter

T7 Promoter

Available since

Jan. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pET28a-hfYFP-TEV-mScarlet-i

Plasmid

#191196

Purpose

Fusion of hfYFP and mScarlet-I separated by a TEV cleavage site for fluorescence-assisted protein purification

Depositor

Gregory Petsko

Article

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

hfYFP & mScarlet-I fusion

Use

Tags

6xHis and mScarlet-I

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-LSSmGFP-TEV-mScarlet-i

Plasmid

#191199

Purpose

Fusion of LSSmGFP and mScarlet-I separated by a TEV cleavage site for fluorescence-assisted protein purification

Depositor

Gregory Petsko

Article

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

LSSmGFP & mScarlet-I fusion

Use

Tags

6xHis and mScarlet-I

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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R152C USP7 TRAF

Plasmid

#227229

Purpose

Expresses human TRAF-like domain of USP7 with a N-terminal cleavable 6His tag in E. coli

Depositor

Irina Bezsonova

Insert

USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)

Use

Tags

6His

Expression

Bacterial

Mutation

R152C; aa 62-205

Promoter

T7-promoter

Available since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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G392A FL USP7

Plasmid

#227217

Purpose

Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli

Depositor

Irina Bezsonova

Insert

USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)

Use

Tags

6His

Expression

Bacterial

Mutation

G392A

Promoter

T7-promoter

Available since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
N418S FL USP7

Plasmid

#227215

Purpose

Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli

Depositor

Irina Bezsonova

Insert

USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)

Use

Tags

6His

Expression

Bacterial

Mutation

N418S

Promoter

T7-promoter

Available since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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H111Q USP7 TRAF

Plasmid

#227227

Purpose

Expresses human TRAF-like domain of USP7 with a N-terminal cleavable 6His tag in E. coli

Depositor

Irina Bezsonova

Insert

USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)

Use

Tags

6His

Expression

Bacterial

Mutation

H111Q; aa 62-205

Promoter

T7-promoter

Available since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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G392D FL USP7

Plasmid

#227216

Purpose

Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli

Depositor

Irina Bezsonova

Insert

USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)

Use

Tags

6His

Expression

Bacterial

Mutation

G392D

Promoter

T7-promoter

Available since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
K420E FL USP7

Plasmid

#227223

Purpose

Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli

Depositor

Irina Bezsonova

Insert

USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)

Use

Tags

6His

Expression

Bacterial

Mutation

K420E

Promoter

T7-promoter

Available since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
L373F FL USP7

Plasmid

#227220

Purpose

Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli

Depositor

Irina Bezsonova

Insert

USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)

Use

Tags

6His

Expression

Bacterial

Mutation

L373F

Promoter

T7-promoter

Available since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
Y331H FL USP7

Plasmid

#227218

Purpose

Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli

Depositor

Irina Bezsonova

Insert

USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)

Use

Tags

6His

Expression

Bacterial

Mutation

Y331H

Promoter

T7-promoter

Available since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
P273H FL USP7

Plasmid

#227221

Purpose

Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli

Depositor

Irina Bezsonova

Insert

USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)

Use

Tags

6His

Expression

Bacterial

Mutation

P273H

Promoter

T7-promoter

Available since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
M225I FL USP7

Plasmid

#227219

Purpose

Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli

Depositor

Irina Bezsonova

Insert

USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)

Use

Tags

6His

Expression

Bacterial

Mutation

M225I

Promoter

T7-promoter

Available since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
F118L USP7 TRAF

Plasmid

#227228

Purpose

Expresses human TRAF-like domain of USP7 with a N-terminal cleavable 6His tag in E. coli

Depositor

Irina Bezsonova

Insert

USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)

Use

Tags

6His

Expression

Bacterial

Mutation

F118L; aa 62-205

Promoter

T7-promoter

Available since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
L469R FL USP7

Plasmid

#227225

Purpose

Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli

Depositor

Irina Bezsonova

Insert

USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)

Use

Tags

6His

Expression

Bacterial

Mutation

L469R

Promoter

T7-promoter

Available since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
E345K FL USP7

Plasmid

#227224

Purpose

Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli

Depositor

Irina Bezsonova

Insert

USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)

Use

Tags

6His

Expression

Bacterial

Mutation

E345K

Promoter

T7-promoter

Available since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
V485G FL USP7

Plasmid

#227222

Purpose

Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli

Depositor

Irina Bezsonova

Insert

USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)

Use

Tags

6His

Expression

Bacterial

Mutation

V485G

Promoter

T7-promoter

Available since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge