Addgene

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BGGLO QPCR PLASMID

Plasmid

#79905

Purpose

Encodes partial human gamma- and beta-globing cDNA for use as QPCR standard as published in PMID: 14592835..

Depositor

Jeffery Miller

Article

Bhanu et al Blood. 2004 Mar 1;103(5):1929-33. Epub 2

Insert

Human partial HBG and HBB gene

Use

Tags

Expression

Mutation

Promoter

No promoter

Available since

Sept. 6, 2016

Availability

Academic Institutions and Nonprofits only

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mCherry PP1 gamma

Plasmid

#45220

Depositor

Iain Cheeseman, Michael Lampson

Article

Liu et al J Cell Biol. 2010 Mar 22;188(6):809-20.

Insert

mCherry PP1 gamma

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 31, 2013

Availability

Academic Institutions and Nonprofits only
RAD Module G8-C1

Plasmid

#38208

Depositor

Drew Endy

Article

Bonnet et al Proc Natl Acad Sci U S A. 2012 Jun 5;109

Inserts

SET Integrase
Bxb1 Excisonase
RESET Integrase

Use

Synthetic Biology; Biobrick cloning vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2012

Availability

Academic Institutions and Nonprofits only

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pAB168

Plasmid

#101671

Purpose

Opto-T7RNAP*(563-F1), equal expr.

Depositor

Mustafa Khammash

Article

Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

Insert

Opto-T7RNAP*(563-F1), equal expr.

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pBW213ara-hrpS

Plasmid

#61435

Purpose

pBAD18-cm encoding rbs33-hrpS-ter

Depositor

Martin Buck, Baojun Wang

Article

Wang et al Nat Commun. 2011 Oct 18;2:508. doi: 10.1

Insert

rbs33-hrpS-ter

Use

Tags

Expression

Bacterial

Mutation

Promoter

pBAD

Available since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pSADdeltaG-GFP-ER(T2)CreER(T2)

Plasmid

#32649

Depositor

Edward Callaway

Article

Osakada et al Neuron. 2011 Aug 25;71(4):617-31.

Inserts

GFP
ER(T2)CreER(T2)

Use

Rabies virus

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 13, 2012

Availability

Academic Institutions and Nonprofits only
pAB170

Plasmid

#101669

Purpose

Opto-T7RNAP*(69), half expr.

Depositor

Mustafa Khammash

Article

Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

Insert

Opto-T7RNAP*(69), half expr.

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 14, 2022

Availability

Academic Institutions and Nonprofits only

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DS-ST1cas

Plasmid

#48647

Purpose

Bacterial S. thermophilus #1 Cas9 (ST1) + tracrRNA expression, cloDF13/spectinomycin

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Inserts

Cas9
ST1 tracrRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

proC

Available since

Nov. 21, 2013

Availability

Academic Institutions and Nonprofits only

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pDB038

Plasmid

#99210

Purpose

Accessory plasmid for PACE of PylRS variants

Depositor

David Liu

Article

Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

Insert

Ppsp [SD8] gIII(P29*), luxAB; PproK pylT

Use

Tags

Expression

Bacterial

Mutation

amber codons at position 29 gIII

Promoter

Ppsp and PproK

Available since

Oct. 16, 2017

Availability

Academic Institutions and Nonprofits only

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pBW115lac-hrpR

Plasmid

#61434

Purpose

pAPT110 encoding rbsH-hrpR-ter

Depositor

Martin Buck, Baojun Wang

Article

Wang et al Nat Commun. 2011 Oct 18;2:508. doi: 10.1

Insert

rbsH-hrpR-ter

Use

Tags

Expression

Bacterial

Mutation

Promoter

pLac

Available since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pDS1928

Plasmid

#167284

Purpose

Expression plasmid for Candida albicans containing a red-shifted luciferase

Depositor

Dominique Sanglard

Article

Dorsaz et al Front Microbiol. 2017 Aug 3;8:1478. doi:

Inserts

red shifted luciferase
SAT1

Use

Tags

Expression

Mutation

Promoter

ACT1

Available since

July 12, 2021

Availability

Academic Institutions and Nonprofits only

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pKDsgRNA-pgaC

Plasmid

#154919

Purpose

aTc inducible gRNA targeting pgaC along with arabinose-inducible lambda red enzymes

Depositor

Michael Lynch

Article

Moreb et al CRISPR J. 2020 Dec;3(6):550-561. doi: 10

Insert

gRNA targeting pgaC

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

pTet

Available since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pMZ288

Plasmid

#59897

Purpose

Combination adh1:cas9/rrk1:sgRNA for CRISPR genome editing in fission yeast: sgRNA targeted against ade6+

Depositor

Mikel Zaratiegui

Article

Jacobs et al Nat Commun. 2014 Oct 29;5:5344. doi: 10.

Inserts

Cas9
rrk1:sgRNA

Use

CRISPR

Tags

Expression

Yeast

Mutation

Silent muation of the CspCI site

Promoter

Available since

Oct. 29, 2014

Availability

Academic Institutions and Nonprofits only

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pDB026a

Plasmid

#109260

Purpose

Accessory plasmid for PACE of MjTyrRS variants

Depositor

David Liu

Article

Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

Insert

Ppsp_pIII(P29*); luxAB; PproK_TyrT(Opt, CUA);

Use

Tags

Expression

Bacterial

Mutation

amber codon at position 29 gIII

Promoter

Ppsp; PproK

Available since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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BBa_J72110-BBa_J72103

Plasmid

#40780

Depositor

Christopher Anderson

Article

Kittleson et al ACS Synth Biol. 2012 Dec 21;1(12):583-9.

Insert

AraC-pBad + coi + cin + repL + pacA

Use

Synthetic Biology; Phagemid

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 30, 2012

Availability

Academic Institutions and Nonprofits only

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pMpGWB102-Citrine-PTS1

Plasmid

#44592

Purpose

Binary vectors for expression of Citrine fluorescent protein with a peroxisome localization signal in plants

Depositor

Yutaka Kodama

Article

Ogasawara et al Plant Cell Environ. 2013 Feb 20. doi: 10

Insert

Citrine-PTS1

Use

Tags

PTS1 (peroxisomal targeting signal type 1)

Expression

Plant

Mutation

Promoter

CaMV 35S promoter

Available since

Sept. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pAM3558

Plasmid

#40252

Depositor

Susan Golden

Article

Chen et al Plasmid. 2008 May;59(3):176-92. Epub 200

Insert

Gm cassette (BssHII+SphI)

Use

Cyanobacteria cloning vector

Tags

Expression

Mutation

Promoter

n/a

Available since

March 25, 2014

Availability

Academic Institutions and Nonprofits only

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pME-TagRFPt-NS

Plasmid

#75343

Purpose

Multisite gateway entry clone for adding N-terminal fusions of TagRFPt

Depositor

Rob Parton

Article

Don et al Zebrafish. 2016 Sep 15.

Insert

TagRFPt

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 17, 2016

Availability

Academic Institutions and Nonprofits only

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XW82

Plasmid

#65840

Purpose

MosSCI-QUAS::Δpes10::GFP

Depositor

Kang Shen

Article

Wei et al Nat Methods. 2012 Mar 11;9(4):391-5. doi

Insert

5XQUAS-delta-pes-10::GFP

Use

Tags

GFP

Expression

Worm

Mutation

Promoter

Available since

Nov. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pAW204

Plasmid

#113244

Purpose

E. coli expression vector for IHFa F13A + IHFb

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

IHFa F13A (ihfA E. coli)
IHFb

Use

Tags

Expression

Bacterial

Mutation

F13A

Promoter

Available since

Aug. 17, 2018

Availability

Academic Institutions and Nonprofits only

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