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Plasmid#211364PurposeLentiviral expression vector for an insert of interest linked to a P2A-T2A puromycin sequence. Produces virus very efficiently. Can also be used for regular transfectionsDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 18, 2024AvailabilityAcademic Institutions and Nonprofits only
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pSL21-VEX
Plasmid#158230PurposeRetroviral expression plasmid of sgRNA with violet-excited fluorescent protein (VEX)DepositorTypeEmpty backboneUseCRISPR and RetroviralTagsExpressionMammalianMutationPromoterU6 promoter for crRNA expression and EFS promoter…Available sinceJan. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pCDH-CB-IRES-copGFP-T2A-Puro
Plasmid#72299PurposeExpress gene of interest under CB promoterDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterchicken beta-actin + CMV enhancerAvailable sinceJan. 25, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pmGFP-P2A-K(AAA)12-P2A-RFP
Plasmid#105687PurposeDouble fluorescent stalling reporter K(AAA)12DepositorInsertsP2A-3xFLAG-VHPbeta
K(AAA)12
P2A-mCherry
UseTagsExpressionMammalianMutationPromoterCMV (from vector)Available sinceFeb. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRK172-6xHis-0N4R-P301S-tau-HiBiT
Plasmid#187835PurposeExpression of 6xHis-tagged P301S tau of 0N4R isoform with a C-terminal HiBiT tag for bimolecular luminescence with LgBiT.DepositorInsert6XHis-0N4R-P301S-tau-HiBiT
UseTags6xHistidine and HiBiTExpressionBacterialMutationPromoterT7Available sinceSept. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
p5-79
Plasmid#118087PurposeN-29-1 split N-terminal T7 RNAP variant-GGSGSGSS-FRB expression plasmidDepositorInsertN-29-1 split N-terminal T7 RNAP variant-GGSGSGSS-FRB expression plasmid
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSNAPf-Cox8A Control Plasmid
Plasmid#101129PurposeExpression of SNAP-Tagged Cox8A in Mammalian cellsDepositorInsertCox8A (COX8A Human)
UseTagsSNAP-tag (SNAPf)ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 27, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSAM_AraC
Plasmid#91569PurposeMariner transposon mutagenesis & sequencing vector - Transposon contains KanR and Illumina sequencing adapters. Arabinose promoter expresses transposase. Ampicillin resistance on backbone.DepositorInsertAraC/PBAD
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
FLHKI-pGFPN3
Plasmid#21917DepositorInsertFull length human HKI in pGFP-N3 (HK1 Human)
UseTagsGFPExpressionMammalianMutationThis is full length human HKI in pGFP-N3PromoterAvailable sinceSept. 17, 2009AvailabilityAcademic Institutions and Nonprofits only -
pLKO5.sgRNA.EFS.tRFP657
Plasmid#57824PurposeLentiviral Vector for Sp sgRNA delivery without SpCas9, tagRFP657, EFS Promoter drivenDepositorInsertssgRNA
EFS
tagRFP657
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceAug. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
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CMV:eGFP-CaM-uTEV1Δ(220-242)
Plasmid#135461PurposeMammalian expression of uFLARE protease componentDepositorInserteGFP-CaM-uTEV1Δ
UseTagseGFP, V5ExpressionMammalianMutationS219V mutation improves stability and S153N impro…PromoterCMVAvailable sinceJan. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1-Luc2-P2A-copGFP
Plasmid#72485PurposeExpress Luc2 and copGFPDepositorInsertLuc2-P2A-copGFP
UseLentiviralTagsExpressionMammalianMutationPromoterEF-1Available sinceJan. 11, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
Flag-HA-USP18
Plasmid#22572DepositorInsertUSP18 (USP18 Human)
UseRetroviralTagsFLAG and HAExpressionMammalianMutationNonePromoterAvailable sinceNov. 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pLenti6-GFP
Plasmid#35637DepositorInsertGFP
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 26, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSL-MS2-6X
Plasmid#27118DepositorInsertMS2 binding sites (6)
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 20, 2010AvailabilityAcademic Institutions and Nonprofits only -
HIP-flash (HOP-flash mutant)
Plasmid#83466PurposeNegative control for HOP-flash. 7xmut TEAD binding sites with minimal promoter plus luciferase reporter geneDepositorInsertMultimerized mutated TEAD binding sites in the promoter of CTGF with minimal promoter (CCN2 Human)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1 CCNE1
Plasmid#164144PurposeDoxycycline inducible expression of Cyclin E1DepositorInsertCCNE1 (CCNE1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 14, 2021AvailabilityAcademic Institutions and Nonprofits only