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Plasmid#219658PurposePermits GoldenGate assembly of new SEED cassettes via Golden Gate assembly (BsaI)DepositorInsertEmpty SEED Cassette (3xP3-dsRed)
UseTagsExpressionMutationPromoterAvailable sinceNov. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGFPC1 MOSPD1
Plasmid#226404PurposeExpression of human MOSPD1 fused to EGFP in mammalian cellsDepositorInsertMOSPD1 (MOSPD1 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hDlx_DIO_ChR2_mCherry
Plasmid#224459PurposeAn AAV vector expressing ChR2-mCherry that is dependent on both Cre and the hDlx enhancerDepositorInsertChR2-mCherry
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR-cBEST-v2-PermE
Plasmid#209446Purposeimproved pCRISPR-cBEST plasmid with optimized sgRNA cloning siteDepositorInsertCodon optimized APOBEC1-nCas9-UGI fusion protein
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterPtipA; sgRNA expression from ermE* promoterAvailable sinceMarch 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
PX458 pSp Cas9 Rhino1 tagA
Plasmid#211627PurposeCas9 Rhino1 tagADepositorInsertRHNO tagA
UseTagsExpressionMammalianMutationPromoterU6Available sinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
MIGR1_FLAG_TAL1-long_GFP
Plasmid#210638PurposeOverexpression of TAL1-longDepositorInsertTAL1-short (TAL1 Human)
UseRetroviralTagsExpressionMutationPromoterAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
ZAK (ZAKA-c057)
Plasmid#98225PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertZAKA (MAP3K20 Human)
UseBaculovirusTagsHis-6-TEVExpressionMutationpfam00069:Pkinase 16-257PromoterpolyhedrinAvailable sinceOct. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
As0
Plasmid#123158PurposeArsenic sensor used in the sensor cell array. pSB4A3 carrying J109-30arsR-t-ParsR-ABS67-30hrpR-30hrpS-t-PhrpLE-30RinA-t-PrinA-30gfp-tDepositorInsertJ109-30arsR-t-ParsR-ABS67-30hrpR-30hrpS-t-PhrpLE-30RinA-t-PrinA-30gfp-t
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ109, ParsR-ABS67, PhrpLE, PrinAAvailable sinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
p28-2iDMD-puro
Plasmid#88895Purpose28°C full-length DMD luc-puro-red plasmid. Co-expresses human DMD, firefly luciferase, puroR and mCherry in mammalian cells. Plasmid carries phiC31 and Bxb1 attB sites, but *cannot* be grown at 37°C.DepositorInsertsUseTagsExpressionMammalianMutationcodon optimizedPromoterAvailable sinceJan. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
TRE:mCherry-p2a- bReaChES
Plasmid#163037PurposeFLiCRE reporter gene for neuronal expressionDepositorInsertmCherry-p2a-bReaChES
UseAAVTagsExpressionMammalianMutationPromoterTREAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMJ922
Plasmid#78312PurposeExpression of His6-MBP-tagged Cas9-NLS-EGFP protein in E. coliDepositorInsertSpCas9
UseTagsHA-2xNLS-EGFP-NLS and His6-MBP-TEVExpressionBacterialMutationhuman codon-optimized gene sequencePromoterT7Available sinceJune 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
L4440_BioBrick-hif-1-sgRNA-A
Plasmid#177784PurposeOverexpression of sgRNAs in E. coli HT115 (targeting C. elegans hif-1 promoter)DepositorInserthif-1 (hif-1 Nematode)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7m-psMEK1tight
Plasmid#89362PurposeA lenti-viral vector expressing photoswitchable MEK1 with minimum basal activity in mammalian cellsDepositorInsertpsMEK1tight (MAP2K1 Synthetic, Human)
UseLentiviralTagsHuman influenza hemagglutinin (HA) tag and pdDron…ExpressionMammalianMutationPromoterCMVAvailable sinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLminP_Luc2P_RE55
Plasmid#90400PurposeC-REL - NFkB gene reporterDepositorInsertFirefly luciferase
UseLentiviralTagsExpressionMutationPromoterCEBPaAvailable sinceNov. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
Beta-2-adrenergic receptor-mRFP
Plasmid#67145PurposeBeta-2-adrenergic receptor tagged with mRFP on carboxyl terminusDepositorInsertBeta-2-adrenergic receptor-mRFP (ADRB2 Discosoma sp., Human)
UseTagsmRFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
Flag-HA-USP13
Plasmid#22568DepositorInsertUSP13 (USP13 Human)
UseRetroviralTagsFLAG and HAExpressionMammalianMutationNonePromoterAvailable sinceDec. 4, 2009AvailabilityAcademic Institutions and Nonprofits only -
pFX-Tom20-mCherry
Plasmid#174188PurposeExpress mCherry with mitochondria-targeting sequence in mammalian cellsDepositorInsertmCherry with mitchondria-targeting sequence
UseTagsthe mitochondria-targeting sequence of TOM20ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pICSL11024 (pICH47732::NOSp-NPTII-OCST)
Plasmid#51144PurposeConfers KAN resistance in plantaDepositorInsertNOSp-NPTII-OCST
UseTagsExpressionMutationPromoterAvailable sinceFeb. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet-1_Ec_rpoZ
Plasmid#128837PurposeE. coli rpoz gene cloned between NcoI and HindIII sites of the first MCS of this Duet plasmid. Omega protein.DepositorInsertrpoZ (rpoZ E. coli)
UseTagsExpressionBacterialMutationPromoterT7Available sinceAug. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
JM110
Bacterial Strain#49763PurposeE.coli K-12 derivativeDepositorBacterial ResistanceStreptomycinAvailable sinceOct. 14, 2014AvailabilityAcademic Institutions and Nonprofits only