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Showing: 10201 - 10220 of 23100 results

  5. pCMV-T7-SpCas9-NG-P2A-EGFP (RTW3677)

    Plasmid
    #139994
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpCas9-NG(L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized SpCas9-NG with BPNLS-3xFLAG-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    SpCas9-NG=L1111R/D1135V/G1218R/E1219F/A1322R/R133…
    Promoter
    CMV and T7
    Available since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    139994-plasmid-map-sequence-id-275519

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  14. pSAH66 - [BAGp | gfp | tbb-2 UTR]

    Plasmid
    #200340
    Purpose
    gfp BioPart for BAG neurons. (1) Mark neurons or (2) cell-specific expression of transgenes (N or C-terminal fusions, or untagged). Compatible with single-copy insertion (MosTI) or arrays.
    Depositor
    Christian Froekjaer-Jensen
    Insert
    [BAGp | gfp | tbb-2 UTR]
    Use
    Tags
    Expression
    Worm
    Mutation
    Promoter
    WBGene00001536, flp-17
    Available since
    Jan. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    200340-plasmid-map-sequence-id-418120

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  15. hMYOD1-3xHA-IRES-hrGFP (#343)

    Plasmid
    #78327
    Purpose
    Overexpression of human MYOD1 ORF with a 3xHA C-terminal tag and an IRES-hrGFP reporter
    Depositor
    Louis Kunkel, Matthew Alexander
    Insert
    MYOD1 (MYOD1 Human)
    Use
    Tags
    3x HA tag and IRES-hrGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 11, 2016
    Availability
    Academic Institutions and Nonprofits only

  17. RhoA sensor (LSS-SGFP2-mCherry)

    Plasmid
    #112948
    Purpose
    RhoA FRET biosensor
    Depositor
    Dorus Gadella
    Insert
    Rhoa sensor
    Use
    Tags
    LSS-SGFP2 and mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    112948-plasmid-map-sequence-id-220190

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  19. pCDH-CB-IRES-copGFP-T2A-Puro

    Plasmid
    #72299
    Purpose
    Express gene of interest under CB promoter
    Depositor
    Kazuhiro Oka
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    chicken beta-actin + CMV enhancer
    Available since
    Jan. 25, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    72299-plasmid-map-sequence-id-131901

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  20. pET28a-RBP-GFP(S45C)-ZdK1

    Plasmid
    #185791
    Purpose
    Encodes green flourescent protein linked to RNA binding peptide (on N terminal end ) and Z domain fragment (variant 1) of Staphylococcal protein A (on C-terminal) via GS-linker
    Depositor
    Lizhong He
    Article
    Younas et al J Phys Chem Lett. 2020 Oct 15;11(20):864
    Insert
    RBP-sfGFP(S45C)-ZdK1
    Use
    Tags
    10xHis, TEV protease cleavage site after His Tag
    Expression
    Bacterial
    Mutation
    Ser to Cys mutation at start of GFP, residue 45 i…
    Promoter
    T7
    Available since
    July 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    185791-plasmid-map-sequence-id-367545

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Showing: 10201 - 10220 of 23100 results