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Plasmid#119558PurposeLevel T acceptor vector for chromosomal integration with lacZ compatible with blue/white screening. Modified pUC19 vector.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only
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pTRANS_231
Plasmid#91120PurposeTransformation, Type: T-DNA, Plant Selection: 2x35S:bar, Viral Replication: BeYDVDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoter35SAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUB_smFLAG_H2B_MS2
Plasmid#81082PurposeTranslation reporter: Encodes Histone H2B (125 aa) fused to smFLAG (Spaghetti Monster FLAG: 10 copies of an epitope tag FLAG).DepositorInsertsmFLAG_H2B_24xMS2
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJG380
Plasmid#91188PurposeBeYDV replicon T-DNA for gene targeting in tobacco leaves, nuclease (AtCas9+gNt_R2+donor)DepositorInsertAtCas9+gNt_R2+donor
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJuly 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-Akt2(WT)
Plasmid#86623PurposeExpresses mCherry-tagged human Akt2(WT)DepositorInsertAkt2 (AKT2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTRANS_211
Plasmid#91110PurposeTransformation, Type: T-DNA, Plant Selection: 2x35S:hpt II, Viral Replication: BeYDVDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoter35SAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-CH111gp160
Plasmid#123268PurposeMammalian expression plasmid for Env from the CH111 HIV-1 isolateDepositorInsertHIV-1 (AD8) Env
UseTagsCD5 leader peptideExpressionMammalianMutationCodon-optimized synthetic genePromoterCMVAvailable sinceMarch 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-flag Mst2 (KR)
Plasmid#27372DepositorInsertMst2 (STK3 Human)
UseTagsflagExpressionMammalianMutationK56R (catalytically inactive)PromoterAvailable sinceFeb. 2, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAR357_SFP_pCDF-1b
Plasmid#122851PurposeCo-expresses the phosphopantheteine transferase Sfp of Bacillus subtilis to transfer a PPant arm to acyl carrier proteins in Escherichia coli. Untagged; in pCDF-1b vector.DepositorInsertSfp
UseTagsExpressionBacterialMutationPromoterT7 promoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Flag-YAP-S109A
Plasmid#33088DepositorInsertYAP (YAP1 )
UseTagsFlagExpressionMammalianMutationS109APromoterCMVAvailable sinceFeb. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMOD_C3006
Plasmid#91096PurposeModule C, Promoter: FMV 34S, Gene: GFP (+ BaeI site for GT donor cloning), Terminator: pea rbcsE9DepositorInsertGFP (+ BaeI site for GT donor cloning)
UseUnspecifiedTagsExpressionMutationPromoterFMV 34SAvailable sinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSMART-GFPuv
Plasmid#65822PurposeGFPuv Expression, Low Phosphate Dependent E. coli ExpressionDepositorInsertgfpuv
UseTagsExpressionBacterialMutationPromoterE . coli insulated waaH gene promoter, low phosp…Available sinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
VPR_dCas9
Plasmid#134601PurposeExpress CHO codon-optimized dCas9 fused to VPR for CRISPR activation. 2A linked ZsGreen1 markerDepositorInsertVPR-dCas9-2A-ZsGreen-DR
UseTagsVPR and ZsGreen1-DRExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pconst #5-RFP
Plasmid#81079Purposeconstitutive promoter expressing RFP, biobrick constructionDepositorInsertRFP
UseP15a origin, constitutive promoter, kanrTagsExpressionMutationPromoterConstitutive Promoter - BBa_J23110Available sinceAug. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
p426 103Q GPD
Plasmid#1184DepositorInserthtt 103Q (HTT Human)
UseTagsGFPExpressionYeastMutationPromoterAvailable sinceMay 25, 2005AvailabilityAcademic Institutions and Nonprofits only -
mEmerald-Vinculin-N-21
Plasmid#54304PurposeLocalization: Focal Adhesions, Excitation: 487, Emission: 509DepositorInsertVinculin (VCL Human)
UseTagsmEmeraldExpressionMammalianMutationPromoterCMVAvailable sinceJune 16, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSUMO-LIC-CHIKV-nsP2h
Plasmid#127935PurposeExpresses CHIKV nsP2h (1-465 aa) in bacterial cellsDepositorInsertCHIKV nsP2h
UseTagsHis6-Sumo-TEVExpressionBacterialMutationPromoterT7 promoterAvailable sinceJuly 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
CAG-mCherry-Otx2shRNA1
Plasmid#73979PurposeOtx2 shRNA1 (mir155 backbone) was inserted into the 3'UTR of mCherry.DepositorInsertshRNA that targets the mouse Otx2 gene
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pA5c-tesA-vhb50-8fadR
Plasmid#86601Purposeexpression of tesA and Vhb50 under the control of a LacUV5 promoter and fadR under the control of a pBAD promoterDepositorInsertstesA
Vhb50
fadR
UseTagsExpressionBacterialMutationPromoterLacUV5 and pBADAvailable sinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A0902
Plasmid#91026PurposeModule A, Promoter: AtUbi10, Gene: TREX2-P2A-AtCas9, Terminator: HSPDepositorInsertTREX2-P2A-AtCas9
UseCRISPRTagsExpressionMutationPromoterAtUbi10Available sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only