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Showing: 10181 - 10200 of 14771 results

  4. pAPV-DsRed2-FLAG-ER-5

    Plasmid
    #170622
    Purpose
    Expresses DsRed2-FLAG targeted to the ER lumen with an N-terminal APV tripeptide after signal peptide cleavage. The protein is efficiently retrieved due to exposure of KDEL.
    Depositor
    Ke Xu
    Article
    Yan et al Dev Cell. 2022 Feb 28;57(4):512-525.e8.
    Insert
    APV-DsRed2-ER-5
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    170622-plasmid-map-sequence-id-335778

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  5. pHB-2

    Plasmid
    #140955
    Purpose
    For PHB production in E. coli (T7-pAC mutant). Please note that this is listed as #140954 in the publication.
    Depositor
    Kang Zhou
    Article
    Liu et al Metab Eng. 2020 Sep;61:206-214. doi: 10.
    Insert
    phaCAB
    Use
    Tags
    Expression
    Bacterial
    Mutation
    R238S mutation in PhaC
    Promoter
    Available since
    April 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    140955-plasmid-map-sequence-id-334168

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  6. mEos3.2-LaminA-C-18

    Plasmid
    #57463
    Purpose
    Localization: Nuclear Envelope, Excitation: 507 / 572, Emission: 516 / 580
    Depositor
    Michael Davidson
    Insert
    LaminA (LMNA Human)
    Use
    Tags
    mEos3.2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 5, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  7. NAT-TET-OFF -LEU- 4xFLAG

    Plasmid
    #182527
    Purpose
    Doxycycline-induced transcriptional regulation and Ubiquitin - LEU - 4xFLAG N-terminal tagging. Ubiquitin is cleaved off, resulting in an unstable protein with an N-terminal leucine.
    Depositor
    Rob Mitra
    Article
    Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102
    Insert
    7 Copies of TetO Box
    Use
    Tags
    4XFLAG
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Jan. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    182527-plasmid-map-sequence-id-358175

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  8. pICSL50015

    Plasmid
    #50318
    Purpose
    Please note- this plasmid is currently not distributed in the MoClo Plant Parts Kit (# 1000000047)
    Depositor
    Nicola Patron
    Article
    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43
    Insert
    CDS, mNEON (Branchistoma lanceolatum)
    Use
    Plant expression
    Tags
    Expression
    Mutation
    BsaI/BbsI sites removed by point-mutation
    Promoter
    Available since
    Feb. 18, 2014
    Availability
    Academic Institutions and Nonprofits only
    50318-plasmid-map-sequence-id-75075

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  11. pBlue-TAL

    Plasmid
    #49401
    Purpose
    The pBlue-TAL is suitable for TALEN mutagenesis in insects. It is compatible with Golden Gate Cloning Kit. It has Esp3I cleavage sites and a T7 promoter.
    Depositor
    Michal Zurovec
    Article
    Takasu et al PLoS One. 2013 Sep 18;8(9):e73458. doi:
    Type
    Empty backbone
    Use
    TALEN
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Available since
    Dec. 19, 2013
    Availability
    Academic Institutions and Nonprofits only
    49401-plasmid-map-sequence-id-73425

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  12. pHelper_O

    Plasmid
    #173783
    Purpose
    The 186 helper plasmid necessary for genomic integration of the pIT5_KO plasmid.
    Depositor
    Keith Shearwin
    Article
    Hao et al ACS Synth Biol. 2021 Jun 30. doi: 10.102
    Insert
    186 Integrase
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    E59G - please see depositor comments
    Promoter
    Available since
    Dec. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    173783-plasmid-map-sequence-id-342367

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  15. pGBW-m4046538

    Plasmid
    #149128
    Purpose
    Yeast Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    Use
    Tags
    Cleavable thrombin;6xHIS
    Expression
    Yeast
    Mutation
    wt
    Promoter
    pTEF1
    Available since
    May 5, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149128-plasmid-map-sequence-id-296274

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  16. pGBW-m4252845

    Plasmid
    #153787
    Purpose
    Mammalian expression plasmid for SARS-CoV-2 S (surface glycoprotein)
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;6xHis-003
    Expression
    Mammalian
    Mutation
    wt
    Promoter
    CMV
    Available since
    June 12, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    153787-plasmid-map-sequence-id-307368

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  19. pSCW02

    Plasmid
    #72301
    Purpose
    Starting substrate for in vitro DNA mismatch repair substrate monitored by FauI cleavage. Derived from pUC19CPDrev H. HWang and J.B. Hays 2002 JBC 277:26136.
    Depositor
    Peggy Hsieh
    Article
    Geng et al Anal Biochem. 2011 Jun 15;413(2):179-84.
    Type
    Empty backbone
    Use
    Derivative of puc19
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    lac
    Available since
    Aug. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    72301-plasmid-map-sequence-id-255198

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  20. pGBW-m4046373

    Plasmid
    #145555
    Purpose
    Yeast Expression plasmid for SARS-CoV-2 ORF3a protein
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 ORF3a protein (ORF3a Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    Use
    Tags
    Cleavable thrombin;StrepII
    Expression
    Yeast
    Mutation
    Saccharomyces cerevisiae recode 1
    Promoter
    pGAL1
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145555-plasmid-map-sequence-id-384616

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Showing: 10181 - 10200 of 14771 results