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Plasmid#218113PurposeIntegration backbone for the MET15 integration site of S. cerevisiae. Includes a HygromycinR gene and a sfGFP drop-out.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only
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pJUMP18-Uac
Plasmid#126959PurposeUniversal Acceptor Plasmid. The fusion sites of the basic part generated are introduced during domestication. sfGFP reporter gene. High copy number oriV #8.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationNonePromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCTCON2-GFP
Plasmid#158130PurposeSingle fluorescent protein reporter (GFP) for ncAA incorporation (wild-type construct)DepositorInsertsfGFP
UseTagsExpressionYeastMutationPromoterGAL1-10Available sinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBL4
Plasmid#50548PurposeExpresses CcaS and CcaR constitutively, and sfGFP/TetR under the PcpcG2 promoterDepositorInsertsCcaS
CcaR
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJune 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pOPS0359
Plasmid#115508PurposeReporter Plasmid. Taurine-inducible expression of GFPDepositorInsertsfGFP pOGG037, T-pharma pOGG003 assembled in pOGG024
UseTagsExpressionBacterialMutationDomesticated for Golden Gate cloningPromoterTaurine-inducible promoter of S. meliloti Promote…Available sinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCTCON2-GYB
Plasmid#160927PurposeDual fluorescent protein reporter (GFP-BFP) for ncAA incorporation (wild-type construct)DepositorInsertsBFP
sfGFP
UseTagsExpressionYeastMutationPromoterGAL1-10Available sinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJMP2367
Plasmid#160076PurposeZ. mobilis CRISPRi, promoter C, empty guide, sfGFPDepositorInsertempty sgRNA
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGC4780
Plasmid#113408PurposeTranslation reporter with inducible translation coupling (high native GFP expression)DepositorInsert158_33121123_1
UseSynthetic BiologyTagsfused to codon deptimized sfGFP with Flexible lin…ExpressionBacterialMutationPromoterAvailable sinceApril 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGC4785
Plasmid#113409PurposeTranslation reporter with inducible translation coupling (low native GFP expression, high under coupling)DepositorInsert359_31121113_2
UseSynthetic BiologyTagsfused to codon deptimized sfGFP with Flexible lin…ExpressionBacterialMutationPromoterAvailable sinceApril 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBEL2957
Plasmid#222972PurposeTo express a putative signal peptide from Encephalitozoon intestinalis protein Eint_060150 fused to GFP in mammalian cellsDepositorInsertsfGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBEL2999
Plasmid#222973PurposeTo express a putative signal peptide from Encephalitozoon intestinalis protein Eint_060140 fused to GFP in mammalian cellsDepositorInsertsfGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBEL3000
Plasmid#222974PurposeTo express a putative signal peptide from Encephalitozoon intestinalis protein Eint_111330 fused to GFP in mammalian cellsDepositorInsertsfGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBEL3001
Plasmid#222975PurposeTo express a putative signal peptide from Encephalitozoon intestinalis protein Eint_071050 fused to GFP in mammalian cellsDepositorInsertsfGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBEL3002
Plasmid#222976PurposeTo express a putative signal peptide from Encephalitozoon intestinalis protein Eint_071040 fused to GFP in mammalian cellsDepositorInsertsfGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBEL3003
Plasmid#222977PurposeTo express a putative signal peptide from Encephalitozoon intestinalis protein Eint_081670 fused to GFP in mammalian cellsDepositorInsertsfGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBEL3004
Plasmid#222978PurposeTo express a putative signal peptide from Encephalitozoon intestinalis protein Eint_081980 fused to GFP in mammalian cellsDepositorInsertsfGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBEL3005
Plasmid#222979PurposeTo express a putative signal peptide from Encephalitozoon intestinalis protein Eint_081970 fused to GFP in mammalian cellsDepositorInsertsfGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBEL3006
Plasmid#222980PurposeTo express a putative signal peptide from Encephalitozoon intestinalis protein Eint_081680 fused to GFP in mammalian cellsDepositorInsertsfGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBEL3007
Plasmid#222981PurposeTo express a putative signal peptide from Encephalitozoon intestinalis protein Eint_081460 fused to GFP in mammalian cellsDepositorInsertsfGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBEL3008
Plasmid#222982PurposeTo express a putative signal peptide from Encephalitozoon intestinalis protein Eint_071030 fused to GFP in mammalian cellsDepositorInsertsfGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 9, 2024AvailabilityAcademic Institutions and Nonprofits only