-
Plasmid#111271Purposeexpress murine Syk tandem SH2 domains (Ser 8 to Gln 264), with Y130E mutation to mimic phosphorylation, in E.coli strain Rosetta 2 (DE3)DepositorInsertmurine Syk tandem SH2 domains (Ser 8 to Gln 264), with Y130E mutation to mimic phosphorylation (Syk Mouse)
UseTagsExpressionBacterialMutationY130E mutation to mimic phosphorylationPromoterT7 promoterAvailable sinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGL2-MCT2Prom0.6
Plasmid#87353PurposeHuman monocarboxylate transporter 2 (MCT2) promoter 0.6 kbpDepositorInsertMCT2 (SLC16A7 Human)
UseLuciferaseTagsExpressionBacterial and MammalianMutationPromoter0.6 kbp proximal promoter of human MCT2 (nested d…Available sinceMarch 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSLQ2827 pHR: U6-SpsgSV40 CMV-PYL1-KRAB-IRES-mCherry
Plasmid#84260PurposeExpresses Sp sgSV40 gRNA with ABA-inducible KRAB and mCherry for diametric regulationDepositorInsertsSp sgSV40
PYL1-KRAB
UseCRISPR and LentiviralTagsIRES-mCherry and PYL1ExpressionMammalianMutationPromoterCMV and mouse U6Available sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEGFPm-DAD M1041A
Plasmid#25411DepositorInsertDiap3 (Diaph3 Mouse)
UseTagsEGFP, His, and MycExpressionMammalianMutationAlanine substitution at critical residue in DAD t…PromoterAvailable sinceOct. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
p-miR-148a
Plasmid#65052PurposeExpression of miR148a in mammalian cellsDepositorInserthsa-miR-148a (pre-miRNA) (MIR148A Human)
UseRetroviralTagsExpressionMammalianMutationPromoterSV40Available sinceJune 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHR-DHFRY100I-sfGFP-NLS-P2A-NLS-mCherry-P2A_ ARHGAP5 5' and 3'UTR
Plasmid#67931PurposeTranslational reporter - ARHGAP5 UTRsDepositorInsertsfGFP-NLS-P2A-NLS-mCherry (ARHGAP5 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAdEasy-mRFP-gsk3 beta S9A
Plasmid#24521DepositorInsertGSK3beta(S9A) (GSK3B Human)
UseAdenoviralTagsmRFPExpressionMammalianMutationS9A. Mutant version of GSK3 that lacks the inhibi…PromoterAvailable sinceJune 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
p-miR-216a
Plasmid#65054PurposeExpression of miR216a in mammalian cellsDepositorInserthsa-miR-216a (pre-miRNA) (MIR216A Human)
UseRetroviralTagsExpressionMammalianMutationPromoterSV40Available sinceJune 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-CAML-Myc-His
Plasmid#22295DepositorInsertcalcium modulating ligand (CAMLG Human)
UseTagsMyc-HisExpressionMammalianMutationPromoterAvailable sinceDec. 2, 2009AvailabilityAcademic Institutions and Nonprofits only -
pGEX2T AtFC1
Plasmid#136363PurposeBacterial expression of recombinant Arabidopsis thaliana ferrochelatase1DepositorInsertFull-length cDNA of Arabidopsis thaliana ferrochelatase1 (FC1 Mustard Weed)
UseTagsGSTExpressionBacterialMutationPromoterLacAvailable sinceJan. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 AK3 Hyg
Plasmid#37539DepositorInsertAK3 (AK3 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 14, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MS2CP-VPg(FCV)
Plasmid#167314PurposeTemplate pDNA to prepare CaVT mRNADepositorInsertCaVT
UseSynthetic BiologyTagsDYK-tagExpressionMammalianMutationPromoterCMVAvailable sinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
-
pAdEasy-EGFP-CLASP2 340-1084
Plasmid#24524DepositorInsertCLASP2 (340-1084) (CLASP2 Human)
UseAdenoviralTagsEGFPExpressionMammalianMutationCLASP2 deletion mutant that retains MT lattice bi…PromoterAvailable sinceSept. 20, 2010AvailabilityAcademic Institutions and Nonprofits only -
-
-
-
pXD70DA7-DadR/DadS-Pdadh-DadhABC(A2)
Plasmid#191630PurposeE. coli - Eggerthella lenta shuttle plasmid (KanR), native promoter-dopamine dehydroxylase from dopamine-metabolizing E. lenta A2 strain with transcriptional regulators DadR/DadSDepositorInsertDopamine dehydroxylase
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXD70Tet(DadHR)-DadR-Pdadh-DadhABC(A2)
Plasmid#191645PurposeE. coli - Eggerthella lenta shuttle plasmid (TetR), native promoter-dopamine dehydroxylase from dopamine-metabolizing E. lenta A2 strain with transcriptional regulator DadRDepositorInsertDopamine dehydroxylase
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXD71Cas10.6RT4-Pct5.1-crRNA(hcdR)-RT(ΔhcdR)
Plasmid#191646PurposeE. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(hcdR-targeting spacer) hcdR-deleting repair template, used for hcdR deletionDepositorInsertCymR
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only