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Plasmid#158533PurposewrmScarlet11 x3 tandem repeatsDepositorInsertwrmScarlet11 x3 tandem repeats
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pC-GoldyTALEN
Plasmid#38143Purposedestination vector for the Golden Gate TALEN kit, directs expression of TALENs from a truncated CAGs promoterDepositorInsertGoldyTALEN
UseTagsAcV5 and FokI homodimerExpressionMammalianMutationTruncate at N and C terminousPromoterminiCaggsAvailable sinceJuly 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
RCIscript-GoldyTALEN
Plasmid#38142Purposedestination vector for the Golden Gate TALEN kit, for in vitro synthesis of TALEN mRNAsDepositorInsertGoldyTALEN
UseMrna transcription vectorTagsAcV5 and FokI homodimerExpressionMutationTruncate at N and C terminousPromoterT3Available sinceSept. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pmEGFP-N1 SYS1
Plasmid#175092PurposeSYS1-mEGFP can be transfected in mammalian cellsDepositorInsertSYS1 (SYS1 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGLOW77
Plasmid#177338PurposeC. elegans mNeonGreen co-injection markerDepositorInsertPmyo-2::mNeonGreen
UseTagsmNeonGreenExpressionWormMutationPromotermyo-2Available sinceJan. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGLOW79
Plasmid#177339PurposeC. elegans mNeonGreen co-injection markerDepositorInsertPmyo-3::mNeonGreen
UseTagsmNeonGreenExpressionWormMutationPromotermyo-3Available sinceJan. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-RCC
Plasmid#190635PurposeMammalian expression of genetically encoded calcium indicator RCCDepositorInsertRCC
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1-Neurod1-3xT7
Plasmid#200890PurposeInducible lentiviral expression of Neurod1DepositorInsertNeurod1 (Neurod1 Mouse)
UseLentiviral; Doxycycline inducibleTags3xT7ExpressionMammalianMutationPromoterTRE promoter, Tet ONAvailable sinceJune 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1-Cdx2-3xT7
Plasmid#200891PurposeInducible lentiviral expression of Cdx2DepositorInsertCdx2 (Cdx2 Mouse)
UseLentiviral; Doxycycline inducibleTags3xT7ExpressionMammalianMutationPromoterTRE promoter, Tet ONAvailable sinceJune 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGD-γb
Plasmid#196327Purpose35S promoter-driven expression of the barley stripe mosaic virus γb RNA silencing suppressorDepositorInsertbarley stripe mosaic virus γb
UseTagsExpressionPlantMutationPromoterduplicated cauliflower mosaic virus 35S promoterAvailable sinceMay 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHiFi Cas9-2×sgRNA (empty, donor)
Plasmid#162277PurposeAll-in-one vector for CRISPR/Cas9-mediated homology-independent knock-in system. The plasmid contains BPNLS-HiFi Cas9-BPNLS and two sgRNA expression cassettes.DepositorInsertHiFi Cas9
UseCRISPRTagsBPNLS and FLAG tagExpressionMammalianMutationSpCas9 (R691A)PromoterAvailable sinceAug. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1-Eomes-3xT7
Plasmid#200888PurposeInducible lentiviral expression of EomesDepositorInsertEomes (Eomes Mouse)
UseLentiviral; Doxycycline inducibleTags3xT7ExpressionMammalianMutationPromoterTRE promoter, Tet ONAvailable sinceJune 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1-Gata6-3xT7
Plasmid#200889PurposeInducible lentiviral expression of Gata6DepositorInsertGata6 (Gata6 Mouse)
UseLentiviral; Doxycycline inducibleTags3xT7ExpressionMammalianMutationPromoterTRE promoter, Tet ONAvailable sinceJune 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lenti-RNF2-ngRNA+41_EF1a-puroR
Plasmid#207358PurposeLentiviral transfer plasmid encoding hU6-driven expression of a RNF2 nicking guide RNA and EF1a-driven puromycin resistance gene.DepositorInsertRNF2 +41 ngRNA
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
Lenti-CMV-PEmax-IRES-ZeoR
Plasmid#207353PurposeLentiviral transfer plasmid containing CMV-driven expression cassette encoding PEmax enzyme for prime editing.DepositorInsertPrime editor max (PEmax) enzyme
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
Lenti-RNF2-tevopreQ1-epegRNA+5G>T_EF1a-puroR
Plasmid#207355PurposeLentiviral transfer plasmid encoding hU6-driven expression of a RNF2 engineered prime editing guide (epegRNA) and EF1a-driven puromycin resistance gene.DepositorInsertRNF2 + 5G>T tevopreq1-prime editing guide RNA (epegRNA)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
Lenti-CMV-PE2-IRES-ZeoR
Plasmid#207352PurposeLentiviral transfer plasmid containing CMV-driven expression cassette encoding PE2 enzyme for prime editing.DepositorInsertPrime editor 2 (PE2) enzyme
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
Lenti-N1303K-ngRNA+14_EF1a-puroR
Plasmid#207359PurposeLentiviral transfer plasmid encoding hU6-driven expression of a ngRNA for correction of N1303K-CFTR via prime editing and EF1a-driven puromycin resistance gene.DepositorInsertN1303K-CFTR +14 ngRNA
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
Lenti-L227R-ngRNA+15_EF1a-puroR
Plasmid#207360PurposeLentiviral transfer plasmid encoding hU6-driven expression of a ngRNA for correction of L227R-CFTR via prime editing and EF1a-driven puromycin resistance gene.DepositorInsertL227R-CFTR +15 ngRNA
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
Lenti-L227R-tevopreQ1-epegRNA+13C>A_EF1a-puroR (PBS 10 - RTT 19)
Plasmid#207357PurposeLentiviral transfer plasmid encoding hU6-driven expression of a L227R-CFTR correcting prime editing guide (epegRNA) and EF1a-driven puromycin resistance gene.DepositorInsertL227R-CFTR + 13 C>A tevopreq1-prime editing guide RNA (epegRNA)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only