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pSCW01
Plasmid#72300PurposeStarting substrate for in vitro DNA mismatch repair substrate monitored by PstI cleavage. Derived from pUC19CPDrev H. HWang and J.B. Hays 2002 JBC 277:26136.DepositorTypeEmpty backboneUseDerivative of puc19TagsExpressionBacterialMutationPromoterlacAvailable sinceAug. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
[GG][Pin]
Plasmid#97191PurposePin nuclease domain with Pum cloning siteDepositorInsertPin domain
UseSynthetic BiologyTagsPum cloning siteExpressionMammalianMutationPromoterCMVAvailable sinceNov. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCY 3010-02
Plasmid#36210DepositorInsertyeast-enhanced (yE) polylinker
UseYeast cassette for pcr and homologous recombinati…TagsyEpolylinker-Cerulean-hphMX4ExpressionMutationPromoterAvailable sinceJuly 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
Talin Head
Plasmid#186140PurposeFor expression of recombinant Talin head domain in bacteriaDepositorInsertTLN1 (TLN1 Human)
UseTagsHis6 (tev cleavage)ExpressionBacterialMutationPromoterLacAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET15-MTFmt
Plasmid#31174DepositorInsertMature form bovine mitochondrial methionyl-tRNA transformylase (MTFMT Bovine)
UseTagsHis and thrombin cleavageExpressionBacterialMutationPromoterAvailable sinceSept. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMEXC3GH (Hyg)
Plasmid#110082PurposeAnalogous to pMINTC3GH but containing oriM for episomal replication in mycobacteria.DepositorTypeEmpty backboneUseTags3C cleavage site - GFP - His10 TagExpressionBacterialMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
PA-mCherry1-LaminA-C-18
Plasmid#57185PurposeLocalization: Nuclear Envelope, Excitation: 400 / 504, Emission: 515 / 517DepositorInsertLaminA (LMNA Human)
UseTagsPA-mCherry1ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
CLAPon
Plasmid#213405PurposePeroxidase (APEX) reporter for TEV protease cleavageDepositorInsertE5-TEVcs-APEX(201-250)-K5-EGFP-APEX(2-200)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
UAS-Syt::3xHalo7
Plasmid#87649PurposeFor creating drosophila transgenics expressing UAS-Syt::3xHalo7DepositorInsertSyt::3xHalo7
UseTagsExpressionInsectMutationS265G in Syt (please see depositor's commen…PromoterAvailable sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
14UAS zfPSD95:GFP
Plasmid#74314PurposeA fusion of zebrafish PSD95 to GFP under control of 14 UAS repeatsDepositorInsertzebrafish PSD95
UseTagsEGFPExpressionMutationY599H and I793S (please see depositor comment bel…PromoterUASAvailable sinceMay 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMEXC3GH (Kan)
Plasmid#110081PurposeAnalogous to pMINTC3GH but containing oriM for episomal replication in mycobacteria.DepositorTypeEmpty backboneUseTags3C cleavage site - GFP - His10 TagExpressionBacterialMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRSETA_crE6G2
Plasmid#172929PurposeCrowding FRET sensor with mCerulean3 and mCitrine for E. coli expression. Linker comprises 2 helices, and a 6AA GSG domainDepositorInsertCrowdingSensorE6G2
UseTagsHisExpressionBacterialMutationVal317Gly, Met316GlyPromoterT7Available sinceAug. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
CHD1 (CHD1A-c107)
Plasmid#110253PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertCHD1 (CHD1 Human)
UseTagsHis-TEVExpressionBacterialMutationPromoterT7 - lacOAvailable sinceJune 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pVITRO1-iRFP702-LaminB1
Plasmid#184456PurposeNuclear membrane-targeted iRFP702 expression in mammalian cellsDepositorInsertiRFP702-LaminB1
UseTagsExpressionMammalianMutationWTPromoterRat EF1αAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
BRD7 (BRD7A-c005)
Plasmid#98245PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertBRD7A (BRD7 Human)
UseTagsHis-6-TEVExpressionBacterialMutationpfam00439:Bromodomain 136-223PromoterT7Available sinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSL690
Plasmid#47753PurposeExpresses dCas9-VP64 fusionDepositorInsertdCas9-VP64
UseCRISPRTags3x FLAG, NLS, and VP64ExpressionMammalianMutationD10A and H840A mutations in Cas9PromoterCMVAvailable sinceAug. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
CHIKV-nsP3-c001
Plasmid#173086PurposeExpression of recombinant fusion protein in E. coliDepositorInsertN/A
UseTagsmCeruleanExpressionBacterialMutationA1334-T1493, codon optimizedPromoterAvailable sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21a - TR
Plasmid#89621Purpose[Edit] Expresses recombinant 6HIS tagged T. cruzi trypanothione reductase protein in E. coli upon IPTG inductionDepositorInsertThioredoxin
UseTags6HIS and T7 tag (gene 10 leader)ExpressionBacterialMutationPromoterAvailable sinceMay 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
PH-dL5**
Plasmid#96946PurposePLC pleckstrin homology domain linked to FAP for CALI validationDepositorInsertPleckstrin homology domain
UseTagsdL5 and mCeruleanExpressionMammalianMutationPromoterCMVAvailable sinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only
