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pC0068 PspCas13 (B12) His6-TwinStrep-SUMO-BsaI

Plasmid

#115219

Purpose

Expresses PspCas13b from a SUMO-tagged bacterial expression construct

Depositor

Feng Zhang

Article

Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

Insert

PspCas13

Use

Tags

SUMO

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 21, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pCL-CTIG

Plasmid

#14901

Purpose

3rd generation lentiviral vector expressing tTA-VP16 and EGFP.

Depositor

Inder Verma

Article

Kafri et al Mol Ther. 2000 Jun . 1(6):516-21.

Insert

CMV-tTA-GFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 11, 2007

Availability

Academic Institutions and Nonprofits only

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EGFP-Rubicon

Plasmid

#28022

Depositor

Qing Zhong

Article

Sun et al J Biol Chem. 2011 Jan 7. 286(1):185-91.

Insert

Rubicon (RUBCN Human)

Use

Tags

3xFlag and EGFP

Expression

Mammalian

Mutation

Promoter

Available since

March 22, 2011

Availability

Academic Institutions and Nonprofits only

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Bassik lab Mouse CRISPR-Cas9 Deletion Library - Stress Proteostasis

Pooled Library

#1000000125

Purpose

CRISPR gRNA mouse knockout pooled library - Stress proteostasis

Depositor

Michael Bassik

Available since

May 23, 2018

Availability

Academic Institutions and Nonprofits only
pcDNA3.3_CoV2_501V2

Plasmid

#170449

Purpose

Express SARS-CoV-2 501V2 (South Africa strain / beta strain) spike protein with an 18aa deletion on the C-terminal tail. Used for SARS2 501V2 pseudotyped virus production.

Depositor

David Nemazee

Article

Yuan et al Science. 2021 Aug 13;373(6556):818-823.

Insert

SARS-CoV-2 501V2 spike D18 (S SARS-CoV-2, 501V2 (B.1.351))

Use

Tags

NA

Expression

Mammalian

Mutation

L18F, D80A, D215G, R246I, K417N, E484K, N501Y, D6…

Promoter

CMV

Available since

May 17, 2021

Availability

Academic Institutions and Nonprofits only

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Human Kinase Domain-Focused CRISPR Knockout Library

Pooled Library

#117725

Purpose

gRNAs targeting the kinase domain of human protein kinases

Depositor

Christopher Vakoc

Article

Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

Expression

Mammalian

Species

Homo sapiens

Use

CRISPR and Lentiviral

Available since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only
pdestMB14_sur5p-dCas9-VP64

Plasmid

#177788

Purpose

Overexpression of dCas9-VP64 in C. elegans

Depositor

Michael Ristow

Article

Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

Insert

dCas9-VP64

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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pJYP1

Plasmid

#202406

Purpose

Backbone vector to clone toxic or unstable DNA using dual-control (transcriptional control via naringenin inducible pfdeA promoter - translational control via B12 riboswitch)

Depositor

Remy Loris

Article

Vandierendonck et al Toxins (Basel). 2023 Aug 18;15(8):508. d

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

fdeA

Available since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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Bassik lab Mouse CRISPR-Cas9 Deletion Library - Drug targets, kinases, phosphatases

Pooled Library

#1000000122

Purpose

CRISPR gRNA mouse knockout pooled library - Drug targets, kinases, phosphatases

Depositor

Michael Bassik

Available since

May 23, 2018

Availability

Academic Institutions and Nonprofits only
RAD21 C-tag CRISPR

Plasmid

#140540

Purpose

RAD21 tagging CRISPR

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

RAD21 (RAD21 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pUC-AdEvolve-DEST

Plasmid

#131692

Purpose

A version of AdEvolve-DEST with a high copy origin for easier DNA preparation and transfection.

Depositor

Matthew D. Shoulders

Article

Berman et al J Am Chem Soc. 2018 Dec 26;140(51):18093

Type

Empty backbone

Use

Adenoviral

Tags

Expression

Mutation

Promoter

Available since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-fDIO-GFP-IRES-tTA

Plasmid

#118026

Purpose

AAV vector for sparse and bright labeling in a cell-type specific manner

Depositor

Minmin Luo

Article

Lin et al Nat Methods. 2018 Nov 19. pii: 10.1038/s

Insert

TRE-fDIO-GFP-IRES-tTA

Use

AAV

Tags

Expression

Mutation

See depositor comments below

Promoter

TRE

Available since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

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mCRISPRa-v2, Membrane Proteins (m6), top 5 sgRNAs/gene

Pooled Library

#84003

Purpose

Mouse CRISPRa Pooled Library targeting membrane proteins

Depositor

Jonathan Weissman

Article

Horlbeck et al Elife. 2016 Sep 23;5. pii: e19760. doi:

Available since

Jan. 12, 2017

Availability

Academic Institutions and Nonprofits only
TOM20MTS-mCherry-EGFP-Tet-On

Plasmid

#109016

Purpose

Tet-On construct to induce expression of tandem eGFP-mCherry tagged TOM20MTS (mitochondria

Depositor

Jacob Corn

Article

Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

Insert

TOM20MTS (MTS: mitochondria targeting signal) (TOMM20 Human)

Use

Lentiviral

Tags

mCherry-eGFP

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pDRF1 mBaojin-T2A-mCherry

Plasmid

#219842

Purpose

equimolar expression of mBaojin and mCherry in budding yeast

Depositor

Bas Teusink

Insert

mBaojin-T2A-mCherry

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pRSET-MagLOV

Plasmid

#219957

Purpose

Bacterial expression of MagLOV, a magnetic-field-sensitive fluorescent protein engineered from AsLOV2

Depositor

Andrew York

Article

Frank Hayward et al (2023) doi: 10.5281/zenodo.8137174

Insert

MagLOV

Use

Tags

6xHis

Expression

Bacterial

Mutation

AsLOV2 C450P L496V Q513K G528K D540M

Promoter

T7

Available since

Aug. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pLSSmOrange-C1

Plasmid

#37131

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al J Am Chem Soc. 2012 Apr 24.

Insert

LSSmOrange

Use

Tags

Expression

Mammalian

Mutation

A44V/F84L/W145M/I165D/M167L/G202D compared to mOr…

Promoter

CMV

Available since

June 5, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mSTING

Plasmid

#214156

Purpose

Transient expression of STING

Depositor

Nan Yan

Article

Dobbs et al Cell Host Microbe. 2015 Aug 12;18(2):157

Insert

STING (Sting1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 5, 2024

Availability

Academic Institutions and Nonprofits only

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mEmerald-Integrin-Beta1-N-18

Plasmid

#54129

Purpose

Localization: Integrin/Focal Adhesions, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Insert

Integrin-Beta1 (ITGB1 Human)

Use

Tags

mEmerald

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 16, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV-CamK2a(0.4)-Opto-cytTrkB(E281A)-HA

Plasmid

#180589

Purpose

Opto-cytTrkB

Depositor

Won Do Heo

Article

Hong J et al J Mol Biol. 2020 Feb 14;432(4):815-827.

Insert

TrkB

Use

AAV

Tags

Expression

Mutation

PHR(E281A)

Promoter

Available since

Feb. 28, 2022

Availability

Academic Institutions and Nonprofits only

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