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Plasmid#40194DepositorInsertMex-5 (RNAi resistant) (mex-5 Nematode)
UseTags3xFLAG (DYKDHDGDYKDHDIDYKDDDDK), GFP, and TEV pro…ExpressionWormMutationExon 2 recoded to be RNAi-resistantPromoter4.4 kb mex-5 promoter fragmentAvailable sinceSept. 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFLIP30
Plasmid#65499PurposeFRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneUseTags6His-AFPt9-attR1 and attR2-mCer-cMycExpressionBacterialMutationPromoterT7 promoterAvailable sinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pInducer21 Flag-Sesn3
Plasmid#61866PurposeDox inducible lentivirial expression of Flag-Sesn3 (mouse)DepositorInsertSesn3 (Sesn3 Mouse)
UseTagsFlagExpressionMammalianMutationnonePromoterAvailable sinceJan. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 TFDP2
Plasmid#67088PurposeStandard for cell-free expression benchmarking.DepositorInsertTFDP2 (TFDP2 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFLIP32
Plasmid#65500PurposeFRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneUseTags6His-AFPt9-attR1 and attR2-t7CFPt9-cMycExpressionBacterialMutationPromoterT7 promoterAvailable sinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFLIP39
Plasmid#65507PurposeFRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneUseTags6His-edAFPt9-attR1 and attR2-t7edCFPt9-cMycExpressionBacterialMutationPromoterT7 promoterAvailable sinceJune 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pExp_TRE:TAL21
Plasmid#51795PurposeInducible expression of TAL21 as input repressorDepositorInsertTAL21
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterTREAvailable sinceMarch 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUAST tdTomato Rab35 DN
Plasmid#53499DepositorInsertRab35 (Rab35 Fly)
UseTagstdtomatoExpressionInsectMutationS22NPromoterGAL4Available sinceJune 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUAST tdTomato Rab35 WT
Plasmid#53502DepositorInsertRab35 (Rab35 Fly)
UseTagstdtomatoExpressionInsectMutationPromoterGAL4Available sinceJune 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUAST YFP Rab35 DN
Plasmid#53501DepositorInsertRab35 (Rab35 Fly)
UseTagsYFPExpressionInsectMutationS22NPromoterGAL4Available sinceJune 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUAST YFP Rab35 WT
Plasmid#53500DepositorInsertRab35 (Rab35 Fly)
UseTagsYFPExpressionInsectMutationPromoterGAL4Available sinceJune 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUAST dsRed Rab35 DN
Plasmid#53506DepositorInsertRab35 (Rab35 Fly)
UseTagsdsRedExpressionInsectMutationS22NPromoterGAL4Available sinceJune 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUAST YFP mouse Rab35 WT
Plasmid#53517DepositorInsertRab35 (Rab35 Mouse)
UseTagsYFPExpressionInsectMutationPromoterGAL4Available sinceJune 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUAST 6xmyc Rab35 WT
Plasmid#53503DepositorInsertRab35 (Rab35 Fly)
UseTags6xmycExpressionInsectMutationPromoterGAL4Available sinceJune 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUAST tdTomato mouse Rab35 CA
Plasmid#53512DepositorInsertRab35 (Rab35 Mouse)
UseTagstdtomatoExpressionInsectMutationQ67LPromoterGAL4Available sinceMay 12, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUAST myc Rab35 DN
Plasmid#53504DepositorInsertRab35 (Rab35 Fly)
UseTagsmycExpressionInsectMutationS22NPromoterGAL4Available sinceMay 12, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUAST YFP mouse Rab35 CA
Plasmid#46015DepositorInsertRab35 (Rab35 Mouse)
UseDrosophilaTagsYFPExpressionInsectMutationQ67L (constitutively active)PromoterGAL4Available sinceJuly 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
p35SZ
Plasmid#51513PurposepMDC32 with Zif268:FokI followed by approximately 3kb of filler sequenceDepositorInsertsZif268:FokI
3kb filler sequence
UsePlant expression t-dnaTagsNLSExpressionMutationPromoter2x35SAvailable sinceMay 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLSLZ.D
Plasmid#51496PurposepLSL with Zif268:FokI coding sequence followed by us:NPTII repair template , Rep is not included on this plasmidDepositorInsertsZif268:FokI
us:NPTII repair template
UsePlant t-dna plasmidTagsSV40 NLSExpressionMutationPromotervirion-sense LIR and 2x35SAvailable sinceMay 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAS-1 (perf)
Plasmid#40836PurposeTarget RNAs used to determine complementarity requirements for in vitro tailing and degradationDepositorInsertlet-7–perf
UseMirna reporterTagsEGFPExpressionInsectMutationPromoterActin5CAvailable sinceSept. 4, 2013AvailabilityAcademic Institutions and Nonprofits only