168,814 results

1. pOT_7-lenti-EFS-SS1-BBz-2A-puro

   Plasmid

   #217990

   Purpose

   Expresses anti-Mesothelin CAR (with 4-1BB signaling domain) linked to puromycin resistance via P2A for lentiviral delivery.

   Depositor

   Neville Sanjana

   Article

   Xue et al bioRxiv 2024.11.27.625752

   Insert

   SS1-BBz CAR
   

   Use

   Lentiviral

   Available Since

   Nov. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti6.2_3xFLAG_V5_ mmIfih1_V5

   Plasmid

   #160110

   Purpose

   Expresses murine Ifih1 in mammalian cells

   Depositor

   Rizwan Haq

   Article

   Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

   Insert

   Ifih1 (Ifih1 Mouse)
   

   Use

   Lentiviral

   Tags

   3xFLAG, V5 and V5

   Expression

   Mammalian

   Available Since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAW62.YY1.FKBP.knock-in.mCherry

   Plasmid

   #104370

   Purpose

   For knocking in FKBP F36V P2A mCherry onto YY1

   Depositor

   Richard Young

   Article

   Weintraub et al Cell. 2017 Dec 14;171(7):1573-1588.e28.

   Insert

   FKBP12(F36V) -P2A-mCherry
   

   Available Since

   Dec. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-hSyn-mCherry-sesRNA-smFlag-tTA2-W3SL

   Plasmid

   #192066

   Purpose

   Expression of mCherry, sesRNA in mammalian cells, with smFlag and tTA2 as efRNA.

   Depositor

   Josh Huang

   Article

   Qian et al Nature. 2022 Oct;610(7933):713-721. doi:

   Insert

   sesRNA for Mouse Ctip2
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   hSyn

   Available Since

   Jan. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. GFP shRNA tet pLKO puro

   Plasmid

   #163017

   Purpose

   Control tet-inducible shRNA targeting eGFP

   Depositor

   Mark Rubin

   Article

   Cotter et al Mol Cancer Res. 2021 Jun 4. pii: 1541-77

   Insert

   Enhanced Green Fluorescent Protein
   

   Use

   Lentiviral

   Available Since

   Feb. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCH45 (multiAsCas12a-KRAB lenti)

   Plasmid

   #217330

   Purpose

   Lentiviral expression of multiAsCas12a-KRAB

   Depositor

   Luke Gilbert

   Article

   Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

   Insert

   multiAsCas12a
   

   Use

   CRISPR and Lentiviral

   Tags

   6xMycNLS and HA-SV40NLS-XTEN80-KRAB-P2A-TagBFP2

   Mutation

   R1226A/E174R/S542R/K548R

   Promoter

   UCOE-SFFV

   Available Since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMKV057

   Plasmid

   #133312

   Purpose

   BeYDV viral replicon on T-DNA backbone expressing Firefly Luc+, WUS2 and IPT

   Depositor

   Daniel Voytas

   Article

   Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

   Insert

   Luc+. WUS2, IPT
   

   Expression

   Plant

   Promoter

   AtUbi10, Nos, 35S

   Available Since

   Dec. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMevT

   Plasmid

   #17815

   Depositor

   Jay Keasling

   Article

   Martin et al Nat Biotechnol. 2003 Jul . 21(7):796-802

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   May 16, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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9. pKLV-EF1a-mCherryLuciferase-W

   Plasmid

   #200101

   Purpose

   Lentiviral vector expressing mCherry fused with Luciferase at the C-terminus

   Depositor

   Kosuke Yusa

   Article

   Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

   Insert

   Luciferase
   

   Use

   CRISPR and Lentiviral

   Available Since

   Jan. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pQE-60NA-proinsulin-msGFP2

    Plasmid

    #160466

    Purpose

    Encodes a fusion of human proinsulin and the monomeric superfolder GFP2

    Depositor

    Benjamin Glick

    Article

    Valbuena et al Traffic. 2020 May 15. doi: 10.1111/tra.1

    Insert

    proinsulin-msGFP2
    

    Expression

    Bacterial

    Promoter

    T5

    Available Since

    March 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMRXIP VPS16-GFP

    Plasmid

    #67023

    Purpose

    Human VPS16 ORF was inserted into pMRXIP GFP-N vector

    Depositor

    Noboru Mizushima

    Article

    Jiang et al Mol Biol Cell. 2014 Apr;25(8):1327-37. d

    Insert

    Homo sapiens vacuolar protein sorting 16 homolog (S. cerevisiae) (VPS16) (VPS16 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Promoter

    CMV

    Available Since

    Aug. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

12. EGFP-Rab7A T22N

    Plasmid

    #28048

    Depositor

    Qing Zhong

    Article

    Sun et al Proc Natl Acad Sci U S A. 2010 Nov 9. 10

    Insert

    Rab7A (RAB7A Human)
    

    Tags

    2xHA and EGFP

    Expression

    Mammalian

    Mutation

    T22N

    Available Since

    May 27, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLKO.1 Sox2 HM a

    Plasmid

    #26353

    Depositor

    Matthew Meyerson

    Article

    Bass et al Nat Genet. 2009 Nov . 41(11):1238-42.

    Insert

    Sox2 shRNA
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    Sept. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET-28b-RfxCas13d-His

    Plasmid

    #141322

    Purpose

    Plasmid for bacterial expression and purification of RfxCas13d protein

    Depositor

    Ariel Bazzini, Miguel Angel Moreno-Mateos

    Article

    Kushawah et al Dev Cell. 2020 Sep 28;54(6):805-817.e7.

    Insert

    Rfx-Cas13d
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-Ef1a-DIO-Sun1GFP-WPRE-pA

    Plasmid

    #160141

    Purpose

    Cre- dependent expression of Sun1GFP for nuclei isolation

    Depositor

    Andreas Pfenning

    Article

    Lawler et al J Neurosci. 2020 Nov 10. pii: JNEUROSCI.

    Insert

    Sun1-GFP (Sun1 Mouse)
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    N-truncated: removed amino acids 1-207, retained …

    Promoter

    Ef1a

    Available Since

    March 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKA-144

    Plasmid

    #79839

    Purpose

    Expresses RpBphP1-mCherry-Intersectin1 DHPH domain under CMVd1

    Depositor

    Vladislav Verkhusha

    Article

    Kaberniuk et al Nat Methods. 2016 May 9. doi: 10.1038/nm

    Insert

    RpBphP1-mCherry-Intersectin1 DHPH domain
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    CMVd1

    Available Since

    Aug. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. PX459v3

    Plasmid

    #178799

    Purpose

    SpCas9 with 2A-Puro, and a golden gate cloning backbone for sgRNA. sgRNA scaffold seqeuence has been modified for increased sgRNA expression (v3).

    Depositor

    Paul Thomas

    Article

    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    Cbh (Cas9-2A Puro) and U6 (gRNA)

    Available Since

    Aug. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCK054 pDEST exorh:mCerulean

    Plasmid

    #195985

    Purpose

    Transgenesis backbone

    Depositor

    Christian Mosimann

    Article

    Kemmler et al Development. 2023 Mar 28:dev.201531. doi

    Type

    Empty backbone

    Use

    Other

    Available Since

    May 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcS-pep1-C1C2

    Plasmid

    #178428

    Purpose

    pep1 as cell penetrating peptide fused to EV membrane binging domain (C1C2)

    Depositor

    Masako Harada

    Article

    Komuro et al Nanotheranostics. 2021 Apr 15;5(4):378-3

    Insert

    pep1
    

    Expression

    Mammalian

    Available Since

    March 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. MG1655 Z1 malE

    Bacterial Strain

    #65915

    Purpose

    E. coli MG1655 Z1 host that produces constitutive levels of TetR, LacI and AraC off of the chromosome with additional malE KO

    Depositor

    Keith Tyo

    Article

    Sekar et al PLoS One. 2016 Feb 22;11(2):e0149746. do

    Bacterial Resistance

    None

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only