168,493 results

1. pFBOH-MHL.RING1B

   Plasmid

   #224712

   Purpose

   Expresses N-terminal hexahistidine-tagged human RING1B (RNF2) in insect cells.

   Depositor

   Chen Davidovich

   Article

   Uckelmann et al bioRxiv [Preprint]. 2024 Feb 12:2023.05.

   Insert

   RING1b (RNF2 Human)
   

   Tags

   6x histidine tag

   Expression

   Insect

   Promoter

   Polyhedrin

   Available Since

   Oct. 18, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pLenti-nSpCas9-NoCBE

   Plasmid

   #209038

   Purpose

   Lentiviral vector expressing doxycycline-inducible human codon-optimized SpCas9(D10A)-6xNLS (Cas9 nickase) linked to ssDNA binding domain from RAD51 and uracil DNA glycosylase inhibitor (UGI)

   Depositor

   Thomas Gonatopoulos-Pournatzis

   Article

   Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

   Insert

   nSpCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG-HA

   Expression

   Mammalian

   Mutation

   D10A

   Promoter

   Tight TRE promoter

   Available Since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti-nSpCas9-APOBEC1

   Plasmid

   #209039

   Purpose

   Lentiviral vector expressing doxycycline-inducible human codon-optimized cytosine base editor where APOBEC1 is fused to the N-terminus of SpCas9(D10A)-sDNA Rad51-2xUGI-6xNLS

   Depositor

   Thomas Gonatopoulos-Pournatzis

   Article

   Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

   Insert

   APOBEC1-nSpCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG-HA

   Expression

   Mammalian

   Mutation

   D10A

   Promoter

   Tight TRE promoter

   Available Since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-nSpCas9-BEACON2

   Plasmid

   #209041

   Purpose

   Lentiviral vector expressing doxycycline-inducible human codon-optimized cytosine base editor where BEACON2 is fused to the N-terminus of SpCas9(D10A)-sDNA Rad51-2xUGI-6xNLS

   Depositor

   Thomas Gonatopoulos-Pournatzis

   Article

   Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

   Insert

   BEACON2-nSpCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG-HA

   Expression

   Mammalian

   Mutation

   D10A

   Promoter

   Tight TRE promoter

   Available Since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-nSpCas9-TadA8.20m

   Plasmid

   #209045

   Purpose

   Lentiviral vector expressing doxycycline-inducible human codon-optimized adenine base editor where TadA8.20m is fused to the N-terminus of SpCas9(D10A)-6xNLS

   Depositor

   Thomas Gonatopoulos-Pournatzis

   Article

   Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

   Insert

   TadA8.20m-nSpCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG-HA

   Expression

   Mammalian

   Mutation

   D10A

   Promoter

   Tight TRE promoter

   Available Since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. NO MS-Sensor (35)

   Plasmid

   #199783

   Purpose

   Synthetic gene circuit

   Depositor

   Timothy Lu

   Article

   Inda-Webb et al Nature. 2023 Aug;620(7973):386-392. doi:

   Insert

   NO MS-Sensor
   

   Use

   Synthetic Biology

   Available Since

   July 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. ssRNb-GFP

   Plasmid

   #128791

   Purpose

   Mammalian expression

   Depositor

   Colin Taylor

   Article

   Prole et al BMC Biol. 2019 May 23;17(1):41. doi: 10.

   Insert

   ssRNb-GFP
   

   Expression

   Mammalian

   Available Since

   Feb. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pmiRGLO-GLIS2-201

   Plasmid

   #117331

   Purpose

   Dual luciferase assay for miRNA-targeted UTR

   Depositor

   Volker Busskamp

   Article

   Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

   Insert

   3' UTR GLIS2-201
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Promoter

   Murine PGK-1

   Available Since

   Dec. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. non-targeting control gRNA (BRDN0001147380)

   Plasmid

   #80225

   Purpose

   3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   Non-targeting control gRNA
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Aug. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. MSCV IRES Luciferase

    Plasmid

    #18760

    Depositor

    Scott Lowe

    Type

    Empty backbone

    Use

    Luciferase and Retroviral

    Expression

    Mammalian

    Available Since

    July 3, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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11. CLYBL-(Ef1a-SBP-LNGFR-T2A-mApple)-(CAG-rtTA)-(TRE-hNIL))

    Plasmid

    #105842

    Purpose

    Donor construct for introduction of hNIL factors and SBP-LNGFR for streptavidin bead enrichment

    Depositor

    Michael Ward

    Article

    Fernandopulle et al Curr Protoc Cell Biol. 2018 Jun;79(1):e5

    Inserts

    Neurogenin 2 (NEUROG2 Human)
    ISL LIM Homeobox 1 (ISL1 Human)
    LIM Homeobox 3 (LHX3 Human)
    Optimized mApple
    rtTA3G
    SBP Delta-LNGFR
    

    Use

    CRISPR and TALEN

    Promoter

    CAG, EF-1alpha, and TRE3G

    Available Since

    April 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-CaMKIIa-rsChRmine-oScarlet-WPRE

    Plasmid

    #183522

    Purpose

    Optogenetics

    Depositor

    Karl Deisseroth

    Article

    Kishi et al Cell. 2022 Feb 17;185(4):672-689.e23. do

    Insert

    rsChRmine-oScarlet
    

    Use

    AAV

    Mutation

    I146M/G174S

    Promoter

    CaMKIIa

    Available Since

    May 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pKM201

    Plasmid

    #13078

    Depositor

    Kenan Murphy

    Article

    Murphy et al BMC Mol Biol. 2003 Dec 13;4:11. doi: 10.

    Insert

    Ptac-gam-bet-exo operon
    

    Expression

    Bacterial

    Available Since

    Oct. 7, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBB550

    Plasmid

    #27396

    Depositor

    Bernd Bukau

    Article

    de Marco et al BMC Biotechnol. 2007 Jun 12;7:32. doi: 1

    Insert

    dnaK, dnaJ, GroESL
    

    Expression

    Bacterial

    Available Since

    Sept. 7, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHelper_NoMutL_V2_gentR

    Plasmid

    #205293

    Purpose

    Helper plasmid for ORBIT in E. coli. Expresses RecT for oligo recombineering and Bxb-1 for pInt incorporation. Has sacB for counter selection.

    Depositor

    Scott Saunders

    Article

    Saunders et al bioRxiv 2023

    Insert

    CspRecT / Bxb-1
    

    Use

    Synthetic Biology

    Available Since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGEX6P1-mCherry-Nanobody (LaM-2)

    Plasmid

    #162276

    Purpose

    Expresses GST-tagged anti-mCherry Nanobody (LaM-2) in E. coli cells.

    Depositor

    Kazuhisa Nakayama

    Article

    Ishida et al Hum Mol Genet. 2021 Apr 26;30(3-4):213-2

    Insert

    mCherry-Nanobody (LaM-2)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. ER-SPLICSs-cFAST10

    Plasmid

    #210431

    Purpose

    Expresses ER-targeted split-CFAST10 for Short-Range ER-Mito Contact sites

    Depositor

    Tito Calì

    Insert

    ER-SPLICSs-cFAST10
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Nov. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-HyPer7-MEM

    Plasmid

    #136465

    Purpose

    Mammalian expression of cytosolic side of plasma membrane targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

    Depositor

    Vsevolod Belousov

    Article

    Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

    Insert

    HyPer7
    

    Tags

    Farnselation tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGBdest-GST-PI3Kc1

    Plasmid

    #187992

    Purpose

    Plasmid for the protein expression of GST-3C-ATG14/Vps34/Vps15/BECN1 in a bacterial and insect expression system. Internal_Reference: SMC1326

    Depositor

    Sascha Martens

    Inserts

    ATG14 (ATG14 Human)
    VPS34 (PIK3C3 Human)
    VPS15 (PIK3R4 Human)
    BECN1 (BECN1 Human)
    

    Tags

    GST

    Expression

    Bacterial and Insect

    Available Since

    Feb. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV.CMV.HI.eGFP-Cre.WPRE.SV40 (AAV1)

    Viral Prep

    #105545-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pAAV.CMV.HI.eGFP-Cre.WPRE.SV40 (#105545). In addition to the viral particles, you will also receive purified pAAV.CMV.HI.eGFP-Cre.WPRE.SV40 plasmid DNA. CMV-driven EGFP-Cre expression. These AAV preparations are suitable purity for injection into animals.

    Depositor

    James M. Wilson

    Promoter

    HI

    Tags

    EGFP

    Available Since

    June 29, 2018

    Availability

    Academic Institutions and Nonprofits only