208,756 results

1. pEGFP C3-Lats1

   Plasmid

   #19053

   Depositor

   Marius Sudol

   Article

   Oka et al J Biol Chem. 2008 Oct 10;283(41):27534-2

   Insert

   Large tumor suppressor kinase 1, N mutant (LATS1 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   **

   Available Since

   Sept. 4, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. tubP-FRT>GAL80-6-FRT>

   Plasmid

   #63173

   Purpose

   ubiquitous expression of a "flp-Out" Drosophila codon-optimized GAL80 gene cassette

   Depositor

   Gerald Rubin

   Article

   Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

   Insert

   "Flp-Out" GAL80
   

   Expression

   Insect

   Available Since

   May 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCSC-NGN2-IRES-GFP-T2A-Sox11

   Plasmid

   #90214

   Purpose

   To convert human skin fibroblasts into induced motor neurons (hiMN) in combination with ISL1, LHX3, FGF2 and two small molecules, forskolin and dorsomorphin.

   Depositor

   Chun-Li Zhang

   Article

   Liu et al Cell Rep. 2016 Jan 5;14(1):115-28. doi:

   Insert

   NGN2-IRES-GFP-T2A-Sox11 (NEUROG2, Sox11 Human, Mouse)
   

   Use

   Lentiviral

   Promoter

   CMV

   Available Since

   Aug. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pOpen-MMLV_RT (lack H)

   Plasmid

   #165556

   Purpose

   Single 75 kDa monomer, cDNA synthesis; high enzyme activity and processivity. Comparable to SuperScriptII from Thermo Fisher.

   Depositor

   Drew Endy, Jennifer Molloy, FreeGenes Project

   Insert

   Moloney Murine Leukemia Virus (MMLV) Reverse Transcriptase RNaseH - (lacking RNaseH domain)
   

   Use

   Synthetic Biology

   Available Since

   Aug. 16, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. pHR_TetO_CD19-P2A-mCherry

   Plasmid

   #133803

   Purpose

   repressible expression of CD19 and mCherry in mammalian cells

   Depositor

   Wendell Lim

   Article

   Toda et al Science. 2018 Jul 13;361(6398):156-162.

   Insert

   putative ECD of H. sapiens CD19 (CD19 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   June 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pGPI-SceI

   Plasmid

   #32060

   Purpose

   Construction of unmarked gene deletions. Suicide cloning vector designed to introduce a targeted I-SceI restriction site in the genome of Burkholderia

   Depositor

   Miguel Valvano

   Article

   Flannagan et al Environ Microbiol. 2008 Jun;10(6):1652-6

   Type

   Empty backbone

   Use

   Mutagenesis plasmid

   Available Since

   Aug. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. DH10B-ALT

   Bacterial Strain

   #61151

   Purpose

   DH10B modified to constitutively express araC, lacI, and tetR, integrated at the attB site on the E Coli genome. This is DH10B-ALT-Tet with the tetracycline resistance cassette deleted.

   Depositor

   Matthew Bennett

   Article

   Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

   Bacterial Resistance

   None

   Available Since

   Oct. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

8. EF1a_mCherry_P2A_Hygro

   Plasmid

   #135003

   Purpose

   Lentiviral vector to express mCherry or to clone and express gene of interest, under control of EF1a promoter. Barcodes compatible with detection in single cell RNA-seq may be inserted at HpaI site.

   Depositor

   Prashant Mali

   Article

   Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pUC-L3L2

   Plasmid

   #114019

   Purpose

   Create Entry clones for Gateway LR reactions through simple restriction enzyme based cloning

   Depositor

   Christopher Grefen

   Insert

   ccdB gene, Chloramphenicol resistance gene
   

   Use

   Gateway

   Available Since

   Jan. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. FH-TET3-pEF

    Plasmid

    #49446

    Purpose

    This plasmid expresses full-length human TET3.

    Depositor

    Anjana Rao

    Article

    Ko et al Nature. 2013 May 2;497(7447):122-6. doi:

    Insert

    Tet methylcytosine dioxygenase 3 (TET3 Human)
    

    Tags

    Flag and HA

    Expression

    Mammalian

    Promoter

    EF-1alpha

    Available Since

    Jan. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pUC-L1L4

    Plasmid

    #114018

    Purpose

    Create Entry clones for Gateway LR reactions through simple restriction enzyme based cloning

    Depositor

    Christopher Grefen

    Insert

    ccdB gene, Chloramphenicol resistance gene
    

    Use

    Gateway

    Available Since

    Jan. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pFA6a-TID-HA3-KanMX6

    Plasmid

    #227980

    Purpose

    To insert biotin ligase, TurboID, at the C-terminus of genes; yeast BioID, KanMX6 marker

    Depositor

    Joseph Reese

    Article

    Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

    Insert

    TurboID
    

    Tags

    HA tag

    Expression

    Yeast

    Available Since

    Nov. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pACAGW-ChR2-Venus-AAV (AAV1)

    Viral Prep

    #20071-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pACAGW-ChR2-Venus-AAV (#20071). In addition to the viral particles, you will also receive purified pACAGW-ChR2-Venus-AAV plasmid DNA. CAG-driven, channelrhodopsin fused to Venus for optogenetic activation. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Karel Svoboda

    Article

    Leopoldo et al Nature. 2009 Jan 18.

    Promoter

    CAGG

    Tags

    Venus

    Available Since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

14. pLJM1 flag-cFLIP

    Plasmid

    #211529

    Purpose

    Expresses flag-tagged cFLIP (CFLAR)

    Depositor

    Richard Possemato

    Article

    Nam et al Nat Metab. 2024 Dec;6(12):2338-2353. doi

    Insert

    cFLIP (CFLAR Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    Jan. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. FUW-TetO-Nr5a1

    Plasmid

    #41081

    Depositor

    Rudolf Jaenisch

    Article

    Buganim et al Cell Stem Cell. 2012 Sep 7;11(3):373-86.

    Insert

    Nr5a1 (Nr5a1 Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    TetO + minimal CMV

    Available Since

    Nov. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pmEGFP-DRD2

    Plasmid

    #190752

    Purpose

    Expression of DRD2 with mEGFP tag on N-terminus.

    Depositor

    Dave Piston

    Article

    Foust et al bioRxiv 2021.11.03.467169

    Insert

    DRD2 (DRD2 Human)
    

    Tags

    mEGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEF5B-FRT-GFP-αTAT1[D157N]

    Plasmid

    #27100

    Depositor

    Maxence Nachury

    Article

    Shida et al Proc Natl Acad Sci U S A. 2010 Dec 14;10

    Insert

    αTubulin K40 acetyltransferase (ATAT1 Human)
    

    Tags

    GFP and S-tag

    Expression

    Mammalian

    Mutation

    D157N

    Available Since

    Jan. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Rosa26 mT/mG

    Plasmid

    #17787

    Depositor

    Liqun Luo

    Article

    Muzumdar et al Genesis. 2007 Sep . 45(9):593-605.

    Insert

    Rosa26 mT/mG
    

    Use

    Cre/Lox and Mouse Targeting

    Tags

    membrane tag

    Expression

    Mammalian

    Available Since

    May 14, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLVX-CMV-Galpha-i1 ONE-GO

    Plasmid

    #189735

    Purpose

    GPCR/G protein BRET biosensor

    Depositor

    Mikel Garcia-Marcos

    Article

    Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

    Inserts

    Galpha-i1 ONE-GO
    GNAI1 (Gnai1 Rat)
    

    Use

    Lentiviral

    Available Since

    Jan. 19, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. TFORF2703

    Plasmid

    #144151

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ESRRA (ESRRA Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    April 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge