168,973 results

1. C1A-C1A-EYFP

   Plasmid

   #61155

   Purpose

   Diacylglycerol (DAG) reporter

   Depositor

   Tobias Meyer

   Article

   Tsai et al Nat Cell Biol. 2014 Feb;16(2):133-44. do

   Insert

   C1A-C1A-EYFP, C1A from PKCG (aa 26-89, rat) (Prkcg Rat)
   

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   C1A from PKCG amino acids 26-89

   Promoter

   CMV

   Available Since

   Dec. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-2dV-Camui (T286A)

   Plasmid

   #220367

   Purpose

   T286A phosphodead mutant of pCAG-2dV-Camui

   Depositor

   Ryohei Yasuda

   Article

   Jain et al Nature. 2024 Oct 9. doi: 10.1038/s41586-

   Insert

   mVenus(Y145W)-mVenus(Y145W)-rat CaMK2 alpha(T286A)-mEGFP(A206K) (Camk2a Rat, Synthetic)
   

   Tags

   mEGFP(A206K) and mVenus(Y145W)-mVenus(Y145W)

   Expression

   Mammalian

   Mutation

   changed Threonine 286 to Alanine

   Promoter

   CAG

   Available Since

   Oct. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAV-HAPLN1

   Plasmid

   #200777

   Purpose

   Expresses full-length mouse HAPLN1 fused with cysteine-free GFP (cfGFP) under synapsin promoter

   Depositor

   Alexander Dityatev

   Article

   Ramsaran et al Science. 2023 May 5;380(6644):543-551. d

   Has Service

   AAV8

   Insert

   Hyaluronan And Proteoglycan Link Protein 1 (Hapln1 Mouse)
   

   Use

   AAV

   Tags

   cfGFP

   Promoter

   Synapsin

   Available Since

   June 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. The Mammalian Toolkit

   Plasmid Kit

   #1000000180

   Purpose

   A ready-made extensive library of plasmids that enable Golden Gate high-throughput assembly of genetic circuits that can be delivered to cells in a multitude of ways.

   Depositor

   Hana El-Samad

   Available Since

   March 11, 2022

   Availability

   Academic Institutions and Nonprofits only

5. UBC plasmid

   Plasmid

   #169746

   Purpose

   Unregulated "open-loop" UBC promoter plasmid. This plasmid encodes eGFP to report circuit output levels.

   Depositor

   Francois St-Pierre

   Article

   Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

   Insert

   EGFP
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   UBC

   Available Since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pENTR221-Renilla

   Plasmid

   #79496

   Purpose

   MultiSite Gateway entry clones, second fragment (attL5, attL2) Renilla Luciferase CDS

   Depositor

   Peter Jon Nelson

   Article

   Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

   Insert

   Renilla Luciferase
   

   Use

   Multisite gateway entry clone

   Promoter

   no Promoter

   Available Since

   May 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. miniCMV-iRFP670-24xMS2

   Plasmid

   #238915

   Purpose

   Contains miniCMV promoter expressing iRFP670 mRNA taged 24xMS2 motif

   Depositor

   Jian-Hui Jiang

   Article

   Zhou et al Angew Chem Int Ed Engl. 2025 Apr 10:e202

   Insert

   iRFP670-24xMS2
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   miniCMV (GGTAGGCGTGTACGGTGGGAGGCCTATATAAGCAGAGCT)

   Available Since

   July 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUHD Cdk1 WT HA

   Plasmid

   #27652

   Depositor

   Greg Enders

   Insert

   Cdk1 (CDK1 Human)
   

   Tags

   HA (YDVPDYA)

   Expression

   Mammalian

   Available Since

   March 18, 2011

   Availability

   Academic Institutions and Nonprofits only

9. pJMP1337

   Plasmid

   #119270

   Purpose

   for cloning new sgRNAs

   Depositor

   Carol Gross, Jason Peters, Oren Rosenberg

   Article

   Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

   Insert

   dcas9
   

   Expression

   Bacterial

   Mutation

   D10A and H840A

   Available Since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. myc-NLRC5

    Plasmid

    #37509

    Depositor

    Thomas Kufer

    Article

    Neerincx et al J Immunol. 2012 May 15;188(10):4940-50.

    Insert

    NLRC5 (NLRC5 Human)
    

    Tags

    myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 9, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET21b-Caspase-3

    Plasmid

    #90087

    Purpose

    Expresses wild type caspase 3 in bacterial cells

    Depositor

    Clay Clark

    Article

    Pop et al Biochemistry. 2001 Nov 27;40(47):14224-3

    Insert

    Caspase 3 (CASP3 Human)
    

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Wild type

    Available Since

    May 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA5/FRT/TO_LAMP1_HaloTag9

    Plasmid

    #175541

    Purpose

    Lyzosomal expression of HaloTag9 in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    LAMP1-HaloTag9
    

    Expression

    Mammalian

    Mutation

    HaloTag9 = HaloTag7-Q165H-P174R

    Available Since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA5/FRT/TO_CalR_HaloTag9_KDEL

    Plasmid

    #175540

    Purpose

    Expression of HaloTag9 in the endoplasmic reticulum of mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    CalR-HaloTag9-KDEL
    

    Expression

    Mammalian

    Mutation

    HaloTag9 = HaloTag7-Q165H-P174R

    Available Since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti-Cre-MS2_UbC-NLS-tdMCP-GFP (Cre_tdMCP-GFP)

    Plasmid

    #219537

    Purpose

    a. Expresses Cre recombinase tagged with 23x repeats of MS2 binding sequence under EF1A promotor and b. NLS-tdMCP_GFP reporter under UbC promotor

    Depositor

    Jeffrey Gerst

    Article

    Haimovich et al bioRxiv 2024.11.06.622258

    Inserts

    Cre recombinase (cre )
    NLS-tdMCP-GFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Nov. 11, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pAAV-nEF-ChRmine-mScarlet (AAV8)

    Viral Prep

    #137158-AAV8

    Purpose

    Ready-to-use AAV8 particles produced from pAAV-nEF-ChRmine-mScarlet (#137158). In addition to the viral particles, you will also receive purified pAAV-nEF-ChRmine-mScarlet plasmid DNA. nEF-driven expression of ChRmine-mScarlet. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Karl Deisseroth, INTRSECT 2.0 Project

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Promoter

    nEF

    Tags

    mScarlet

    Available Since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

16. pLenti-C-Myc-DDK-IRES-Puro HEXIM1

    Plasmid

    #200726

    Purpose

    Lentiviral expression of Flag-Tagged human HEXIM1

    Depositor

    Claudio Alarcón

    Insert

    HEXIM1 (HEXIM1 Human)
    

    Use

    Lentiviral

    Tags

    Myc-DDK from backbone plasmid

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDONR221_SLC4A5

    Plasmid

    #132284

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC4A5 (SLC4A5 Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 14, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pT7-CalfluxVTN

    Plasmid

    #83926

    Purpose

    Bioluminescent Reporter of Intracellular Calcium

    Depositor

    Carl Johnson

    Article

    Yang et al Nat Commun. 2016 Oct 27;7:13268. doi: 10

    Insert

    CalfluxVTN
    

    Expression

    Bacterial

    Mutation

    None

    Promoter

    T7

    Available Since

    Oct. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. AiP12558 - pAAV-AiE0390m_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2558)

    Plasmid

    #208141

    Purpose

    AiE0390m_3xC2 is an optimized enhancer sequence designed to drive AAV-mediated transgene expression in brain astrocytes

    Depositor

    Allen Institute for Brain Science, Jonathan Ting

    Article

    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

    Insert

    SYFP2
    

    Use

    AAV

    Mutation

    NA

    Promoter

    Beta Globin minimal promoter

    Available Since

    Oct. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. 13XLexAop2 IVS ChrimsonR mCherry_tr

    Plasmid

    #111548

    Purpose

    Optogenetics in Drosophila: LexAop2-induced expression of ChrimsonR mCherry

    Depositor

    Vivek Jayaraman

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    ChrimsonR_mCherry_trafficked
    

    Expression

    Insect

    Mutation

    wt

    Promoter

    hsp70

    Available Since

    June 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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