294,010 results

1. pCW57.1-FOXM1c

   Plasmid

   #68810

   Purpose

   Doxycycline inducible lentiviral vector for FOXM1c expression in mammalian cells

   Depositor

   Adam Karpf

   Article

   Barger et al Oncotarget. 2015 Jul 16.

   Insert

   FOXM1c (FOXM1 Human)
   

   Use

   Lentiviral; Doxycycline inducible

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   TRE promoter, Tet ON

   Available Since

   Sept. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. CROPseq-puro-v2

   Plasmid

   #127458

   Purpose

   Entry plasmid for guide cloning

   Depositor

   Paul Blainey

   Article

   Feldman et al Cell. 2019 Oct 17;179(3):787-799.e17. do

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   July 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. mU6-CR1-CRISPRiv2_hU6-CR3-NT-ctrl-guide_EF1a-PuroR-T2A-BFP

   Pooled Library

   #197348

   Purpose

   To allow for genome-wide genetic modifier screening, the library has a second fixed non-targeting control guide.

   Depositor

   Rebecca Voorhees, Jonathan Weissman

   Article

   Guna et al BMC Genomics. 2023 Oct 30;24(1):651. doi

   Species

   Homo sapiens

   Use

   Lentiviral

   Available Since

   May 25, 2023

   Availability

   Academic Institutions and Nonprofits only

4. pINDUCER21-puro_Gateway-3xFLAG

   Plasmid

   #172981

   Purpose

   Gateway Destination vector for doxycycline-inducible expression of ORF

   Depositor

   Michael Wehr

   Article

   Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Available Since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-Syn-ChrimsonR-tdT

   Plasmid

   #59171

   Purpose

   AAV-mediated expression of ChrimsonR-tdTomato under the Syn promoter

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Has Service

   AAV Retrograde, AAV1, AAV5, and AAV9

   Insert

   ChrimsonR-tdTomato
   

   Use

   AAV

   Tags

   tdTomato

   Expression

   Mammalian

   Promoter

   Syn

   Available Since

   Aug. 13, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAV-P3-evoCjCas9-ABE8e_gRNA

   Plasmid

   #202559

   Purpose

   P3-evoCjCas9-ABE8e and hU6-BsmBI-gRNA in AAV2 backbone (ITRs)

   Depositor

   Gerald Schwank

   Article

   Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

   Insert

   evoCjCas9-ABE8e
   

   Use

   AAV

   Available Since

   Sept. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3 Myc2- Hs TTP WT

   Plasmid

   #107008

   Purpose

   Expresses epitope-tagged wild-type human TTP

   Depositor

   Julian Downward

   Article

   Coelho et al Immunity. 2017 Dec 19;47(6):1083-1099.e6

   Insert

   tristetraprolin (ZFP36 Human)
   

   Tags

   Double Myc peptide epitope tag

   Expression

   Mammalian

   Available Since

   March 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. mCRISPRa-v2, Membrane Proteins (m6), top 5 sgRNAs/gene

   Pooled Library

   #84003

   Purpose

   Mouse CRISPRa Pooled Library targeting membrane proteins

   Depositor

   Jonathan Weissman

   Article

   Horlbeck et al Elife. 2016 Sep 23;5. pii: e19760. doi:

   Available Since

   Jan. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

9. pC035 - dLwCas13a-msfGFP

   Plasmid

   #91925

   Purpose

   Expresses catalytically inactive LwCas13a for mammalian RNA targeting

   Depositor

   Feng Zhang

   Article

   Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

   Insert

   dLwCas13a
   

   Use

   CRISPR

   Tags

   NLS-3xHA and NLS-msfGFP

   Expression

   Mammalian

   Mutation

   R488A and R1060A

   Promoter

   EF-1a

   Available Since

   Nov. 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. TLCV2-RB1

    Plasmid

    #87836

    Purpose

    LentiCRISPR v2 was modified into an all-in-one dox inducible system. The addition of doxycycline induces Cas9-2A-eGFP. The U6 promoter drives constitutive expression of an sgRNA targeting human RB1.

    Depositor

    Adam Karpf

    Article

    Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:

    Insert

    sgRB1 (RB1 Human)
    

    Use

    CRISPR and Lentiviral; Doxycycline inducible; egf…

    Expression

    Mammalian

    Promoter

    Tight TRE promoter and U6

    Available Since

    April 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRDA_867

    Plasmid

    #231109

    Purpose

    A>G base editor

    Depositor

    John Doench, David Root

    Article

    Kaplan et al Nat Genet. 2025 Nov;57(11):2920-2929. do

    Insert

    ABE8e-nCas9-SpG-HA
    

    Use

    CRISPR and Lentiviral

    Mutation

    D10A

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pC1-SypHer3s

    Plasmid

    #108118

    Purpose

    Ratiometric pH probe with enhanced brightness for mammalian cells expression.

    Depositor

    Vsevolod Belousov

    Article

    Ermakova et al Chem Commun (Camb). 2018 Mar 2. doi: 10.

    Insert

    SypHer3s
    

    Tags

    ratiometric YFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJSC111 - Bacterial expression plasmid for SpCas9-HF1 variant

    Plasmid

    #101209

    Purpose

    Bacterial expression plasmid for SpCas9-HF1 variant

    Depositor

    Jennifer Doudna, Keith Joung

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant N497A/R661A/Q695A/Q926A
    

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    N497A, R661A, Q695A and Q926A

    Promoter

    T7

    Available Since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pU6-tmpknot-GG-acceptor

    Plasmid

    #174039

    Purpose

    Prime editing in mammalian cells

    Depositor

    David Liu

    Article

    Nelson et al Nat Biotechnol. 2021 Oct 4. pii: 10.1038

    Type

    Empty backbone

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Oct. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBG-ttLbUV2

    Plasmid

    #231386

    Purpose

    LbCas12a variant with D156R and E795L mutations and harboring same NLS sequence as PEmax but codon-optimized for dicot species; for Agrobacterium-mediated Arabidopsis transformation.

    Depositor

    Qi-Jun Chen

    Article

    Xin et al aBIOTECH. 2024 Mar 26;5(2):117-126. doi:

    Insert

    ttLbCas12a Ultra V2
    

    Use

    CRISPR

    Expression

    Plant

    Mutation

    D156R and E795L

    Available Since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-HA2-CAND1

    Plasmid

    #20719

    Depositor

    Yue Xiong

    Article

    Liu et al Mol Cell. 2002 Dec . 10(6):1511-8.

    Insert

    CAND1 (cullin-associated and neddylation-dissociated 1) (CAND1 Human)
    

    Tags

    HA2

    Expression

    Mammalian

    Available Since

    April 21, 2009

    Availability

    Academic Institutions and Nonprofits only

17. FR_HNF1B

    Plasmid

    #31101

    Depositor

    Gerhart Ryffel

    Article

    Thomas et al Nucleic Acids Res. 2004 . 32(19):e150.

    Insert

    HNF1B (HNF1B Human)
    

    Use

    Flp/frt

    Tags

    myc

    Expression

    Mammalian

    Mutation

    wild type

    Available Since

    Oct. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAGGs-RFP

    Plasmid

    #127347

    Purpose

    Expresses RFP constitutively via the CAGGS promoter in mammalian cells

    Depositor

    Phil Sharp

    Insert

    Red Fluorescent Protein
    

    Expression

    Mammalian

    Promoter

    CAGGS Promoter

    Available Since

    Dec. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. PE-P3-maize

    Plasmid

    #185611

    Purpose

    For plant prime editing in maize plants

    Depositor

    Jinxiao Yang

    Article

    Xu et al Nat Plants. 2022 Jan;8(1):45-52. doi: 10

    Insert

    3XFLAG-4XNLS-M-MLV-nCas9(H840A)-4XNLS
    

    Use

    CRISPR

    Promoter

    ZmUbi

    Available Since

    Aug. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLV-neo-CD2-FAK

    Plasmid

    #37013

    Depositor

    Bob Weinberg

    Article

    Shibue et al Proc Natl Acad Sci U S A. 2009 Jun 23;10

    Insert

    CD2-FAK (CD2, PTK2 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Oct. 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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