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Plasmid#178362PurposeNuclear localized GCaMP-X sensor for monitoring calcium signal in nucleus.DepositorInsertjGCaMP7b-XN
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV5-AML1B
Plasmid#12426PurposepCMV5-AML1B is the original plasmid used in Meyers et al 1995. However, please note that the insert sequence has now been identified as AML1C.DepositorInsertAML1B (RUNX1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 17, 2006AvailabilityAcademic Institutions and Nonprofits only -
MR015-H11-L2-TALEN
Plasmid#51554PurposeExpresses the H11-L2 TALEN in mammalian cells. This TALEN was coexpressed with H11-R2 (plasmid #51555) to cleave the H11 locus.DepositorInsertH11-L2 TALEN
UseTALENTagsExpressionMammalianMutationPromoterAvailable sinceMarch 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
MR015-H11-R2-TALEN
Plasmid#51555PurposeExpresses the H11-R2 TALEN in mammalian cells. To cleave the H11 locus, this TALEN was coexpressed with H11-L2 (plasmid #51554).DepositorInsertH11-R2 TALEN
UseTALENTagsExpressionMammalianMutationPromoterAvailable sinceMarch 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_IgKchL_HA_HaloTag7_myc_PDGFRtmb
Plasmid#175534PurposeExpression of HaloTag7 at the outer leaflet of the plasma membrane of mammalian cellsDepositorInsertIgKchL_HA-HaloTag7-myc_PDGFRtmb
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pETZZtevPP7CP(C68A,C73A)
Plasmid#27548DepositorInsertPseudomonas phage PP7 coat protein (cp Pseudomonas phage PP7)
UseTagsTEV protease cleavage site, six histidine tag, an…ExpressionBacterialMutationchanged two cysteine to alanine (C68A,C73A) to in…PromoterAvailable sinceMarch 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
mEmerald-PCNA-19
Plasmid#54221PurposeLocalization: Nucleus, Excitation: 487, Emission: 509DepositorInsertPCNA-19 (PCNA Human)
UseTagsmEmeraldExpressionMammalianMutationPromoterCMVAvailable sinceJuly 10, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3-StagRFP
Plasmid#173014PurposeRationally enhanced red fluorescent protein derived from TagRFP-T. For mammalian expression.DepositorInsertStagRFP
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tagExpressionMammalianMutationPromoterCMVAvailable sinceSept. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBS-TarHom-Tol2-kanaR-cryaa-CFP
Plasmid#74151PurposeBAC targeting cassette for pTARBAC2 with kanaR selection marker and lens-specific transgenesis marker CeruleanDepositorInsertTol2-kanaR-cryaa-CFP
UseTagsExpressionMutationPromoterAvailable sinceMarch 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_HaloTag9_LamB1
Plasmid#175538PurposeExpression of HaloTag9 at the nuclear lamina of the mammalian cellsDepositorInsertHaloTag9-LamB1
UseTagsExpressionMammalianMutationHaloTag9 = HaloTag7-Q165H-P174RPromoterAvailable sinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
MXS_P2A
Plasmid#62433PurposeMXS_chaining vector with P2ADepositorInsertSelf-cleaving peptide
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
AiP14496: pAAV-AiE0873m_3xC2-minBG-SYFP2-P2A-3XFLAG-10aa-H2B-WPRE3-BGHpA (Alias: CN4496)
Plasmid#214858PurposeExpression of SYFP2 (yellow fluorescent protein) plus separate nuclear FLAG epitope in striatal cholinergic neuronsHas ServiceAAV PHP.eBInsertSYFP2
UseAAVTagsExpressionMutationPromoterAvailable sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_Lyn11-HaloTag7
Plasmid#175533PurposeExpression of HaloTag7 at the inner leaflet of the plasma membrane of mammalian cellsDepositorInsertLyn11-HaloTag7
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-SpyCatcher002
Plasmid#102827PurposeBacterial expression of SpyCatcher002 for covalent coupling to SpyTag002-linked proteinsDepositorInsertSpyCatcher002
UseTagsGSSGS, His6, and TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceDec. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
mCherry-BAF
Plasmid#172447PurposeExpression of nuclear protein BAF with an N-terminal mCherry tagDepositorInsertBAF
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceApril 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pENTR4-myc-nuc (w556-1)
Plasmid#20026PurposeGateway entry vector for nuclear targeting of domains (C-term NLS, myc tags)DepositorTypeEmpty backboneUseGateway entry vectorTagsMyc and NLSExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-H2B_Halo_T2A_EGFP
Plasmid#135444PurposeMamalian cell expression of nuclear HaloTag7 and cytosolic EGFPDepositorInsertH2B-HaloTag7 and EGFP
UseTagsH2BExpressionMammalianMutationPromoterCMV/TetOAvailable sinceDec. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
Prab-3::NLS::GFP
Plasmid#68120PurposeNuclear Localized GFP for pan-neuronal expression in C. ElegansDepositorInsertGFP
UseTagsExpressionWormMutationPromoterrab-3Available sinceAug. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-OTI-TCRA_p2_TCRB
Plasmid#131033PurposeExpresses OT-I TCRA linked to TCRB with the P2A self-cleaving peptideDepositorArticleUseTagsExpressionMammalianMutationPromoterCMV/T7Available sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only