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Plasmid#92236PurposeExpress D661A mutant of prolyl oligopeptidase from Galerina marginataDepositorInsertProlyl oligopeptidase B from Galerina marginata-D661A mutant
UseTags6x-Histag, TEV cleavage siteExpressionBacterialMutationAspartate 661 to AlaninePromoterT7 promoterAvailable sinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
His8-TEV-PseC
Plasmid#89724PurposeExpresses PseC from C. jejuni with an N-terminal octaHis tag and TEV cleavage siteDepositorInsertPseC (pseC Campylobacter jejuni)
UseTagsHis8 and TEVExpressionBacterialMutationPromoterAvailable sinceFeb. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
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GRM3 (3SM9)
Plasmid#32870PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertGRM3 (GRM3 Human)
UseTagsHis tag with TEV cleavage and Honeybee melittin S…ExpressionBacterialMutationPromoterAvailable sinceJan. 13, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
M18 pCS-twhh-nBeta gal Vec
Plasmid#17155DepositorInserttwhh promoter (shhb Zebrafish)
UseTagsnuclear beta-galExpressionMutationPromoterAvailable sinceFeb. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
pEMS1938
Plasmid#82563PurposeHigh copy number plasmid containing Ple261 (CLDN5 MiniPromoter) insert.DepositorInsertPle261
UsePleiades promoter project [sic, pleaides plieades]TagsExpressionMutationPromoterAvailable sinceSept. 14, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDGE65
Plasmid#79447Purposerecipient plasmid [single nuclease]; pPcUbi:Cas9, BASTADepositorInsertccdB (ccdB(letD) )
UseCRISPRTagsExpressionPlantMutationBsaI site eliminatedPromoterAvailable sinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEG608
Plasmid#40194DepositorInsertMex-5 (RNAi resistant) (mex-5 Nematode)
UseTags3xFLAG (DYKDHDGDYKDHDIDYKDDDDK), GFP, and TEV pro…ExpressionWormMutationExon 2 recoded to be RNAi-resistantPromoter4.4 kb mex-5 promoter fragmentAvailable sinceSept. 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
HECTD1
Plasmid#32860PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertHECTD1 (HECTD1 Human)
UseTagsHis (with TEV cleavage)ExpressionBacterialMutationamino acids 1879-1966 onlyPromoterAvailable sinceJan. 6, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCS2-FAK0
Plasmid#59128PurposeCloning vector for FAK fret sensor without helixDepositorInsertFocal Adhesion Kinase
UseTagsFLAG, mCerulean, and mCitrineExpressionMammalianMutationPromoterCMVAvailable sinceNov. 12, 2014AvailabilityAcademic Institutions and Nonprofits only -
mEmerald-PCNA-19-SV40NLS-4
Plasmid#54223PurposeLocalization: Nucleus, Excitation: 487, Emission: 509DepositorInsertPCNA-19 SV40NLS (PCNA Human)
UseTags3xSV40NLS and mEmeraldExpressionMammalianMutationPromoterCMVAvailable sinceJuly 10, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
CJ41 (U1 snRNA (U1;21d))
Plasmid#17774DepositorInsertU1 snRNA (U1;21d) (snRNA:U1:21D Fly)
UseTagsExpressionMutationPromoterAvailable sinceApril 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
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LI B-C N-NES KOZ b c7
Plasmid#54445DepositorInsertNuclear Export Signal (NES) of heat stable inhibitor (PKI)
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceJune 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
mEos2-LaminA-C-18
Plasmid#57394PurposeLocalization: Nuclear Envelope, Excitation: 505 / 569, Emission: 516 / 581DepositorInsertLaminA (LMNA Human)
UseTagsmEos2ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 5, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
MAC3-C-GFP-NLS
Plasmid#222604PurposeExpression of MAC3-tagged GFP-NLS (C-terminal)DepositorInsertEGFP and 5' nuclear localizaition sequence (NLS) (PPKTTRKVED)
UseTagsMAC3ExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceAug. 6, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
MAC3-N-GFP-NES
Plasmid#222606PurposeExpression of MAC3-tagged GFP-NES (N-terminal)DepositorInsertEGFP and 3' nuclear exclusion sequence (NES)
UseTagsMAC3ExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceAug. 5, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
MAC3-N-GFP-NLS
Plasmid#222607PurposeExpression of MAC3-tagged GFP-NLS (N-terminal)DepositorInsertEGFP and 3' nuclear localizaition sequence (NLS)
UseTagsMAC3ExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceAug. 5, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
MAC3-C-GFP-NES
Plasmid#222603PurposeExpression of MAC3-tagged GFP-NES (C-terminal)DepositorInsertEGFP and 5' nuclear exclusion sequence (NES)
UseTagsMAC3ExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceAug. 5, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
AiP14496: pAAV-AiE0873m_3xC2-minBG-SYFP2-P2A-3XFLAG-10aa-H2B-WPRE3-BGHpA (Alias: CN4496)
Plasmid#214858PurposeExpression of SYFP2 (yellow fluorescent protein) plus separate nuclear FLAG epitope in striatal cholinergic neuronsInsertSYFP2
UseAAVTagsExpressionMutationPromoterAvailable sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only