Addgene

We narrowed to 24,726 results for: sta

Showing: 10001 - 10020 of 24726 results

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pCMV-myc-CD4-Sema4D

Plasmid

#51603

Purpose

expresses Sema4D/CD4 chimera with intracellular Sema4D, extracellular CD4

Depositor

Suzanne Paradis

Article

Raissi et al Mol Cell Neurosci. 2013 Nov;57:23-32. do

Insert

CD4-Sema4D

Use

Tags

myc

Expression

Mammalian

Mutation

Sema4D amino acids 1-754 have been replaced with …

Promoter

CMV

Available since

April 7, 2014

Availability

Academic Institutions and Nonprofits only
pKP4010

Plasmid

#113336

Purpose

Beta-estradiol inducible 3X-FLAG Ty3 Integrase; Z4 promoter-3XFLAG Ty3 IN-CYC1 terminator; artificial transcription factor under ACT1 promoter

Depositor

Suzanne Sandmeyer

Article

Patterson et al Genome Res. 2019 Aug;29(8):1298-1309. do

Inserts

Artificial Transcription Factor (McIsaac et al, 2013)
Ty3 Integrase

Use

Tags

3X-FLAG/8X-Gly linker

Expression

Yeast

Mutation

Promoter

Available since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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psbA2-Ptrc-T7-g10-PHLS (e)

Plasmid

#52310

Purpose

Replaces the psbA2 gene in Synechocystis with the Ptrc promoter-T7-g10-PHLS gene

Depositor

Anastasios Melis

Article

Formighieri et al Planta. 2014 Aug;240(2):309-24. doi: 10.

Inserts

beta-phellandrene synthase
Chloramphenicol resistance

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Ptrc-T7

Available since

June 10, 2014

Availability

Academic Institutions and Nonprofits only

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pCRII-TOPO CMV-cGFP-mMALAT1_3' Comp.14 Sense

Plasmid

#46838

Purpose

Express cGFP transcript ending in the minimal functional MALAT1 triple helix

Depositor

Phil Sharp

Article

Wilusz et al Genes Dev. 2012 Nov 1;26(21):2392-407. d

Insert

Coral Green Fluorescent Protein (cGFP)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 25, 2013

Availability

Academic Institutions and Nonprofits only

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pRRG260-Cyp1A1

Plasmid

#99081

Purpose

dsRNA and riboprobe synthesis for Schmidtea mediterranea Cyp1A1 (also known as h.49.4f)

Depositor

Phillip Newmark

Article

Roberts-Galbraith et al Proc Natl Acad Sci U S A. 2013 Jan 22;11

Insert

Cyp1A1 (also known as h.49.4f) in situ probe

Use

T/a cloning vector for dsrna generation and the g…

Tags

Expression

Mutation

Promoter

Available since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pCEP4-SHIV327C-HuA10-gp160

Plasmid

#123270

Purpose

Mammalian expression plasmid for Env from the SHIV327C-Hu A10 strain

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

SHIV327C-Hu A10 Env

Use

Tags

CD5 leader peptide

Expression

Mammalian

Mutation

Codon-optimized synthetic gene

Promoter

CMV

Available since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

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pVRa38_1322

Plasmid

#49698

Depositor

Christopher Voigt

Article

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

ECF38_1322

Use

Synthetic Biology

Tags

His6-PreScission

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pT7-lacO

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pTC303

Plasmid

#91210

Purpose

protoplast vector expressing 2 gRNAs targeting tomato ARF8A (AtU6 and AtSL7 promoters)

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

2 gRNAs targeting tomato ARF8A

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

July 11, 2017

Availability

Academic Institutions and Nonprofits only

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p230-pOsAct1::hpt-pZmUbi::BdPetC

Plasmid

#129649

Purpose

Rieske FeS overexpression

Depositor

Susanne von Caemmerer

Article

Ermakova et al Commun Biol. 2019 Aug 16;2:314. doi: 10.

Insert

CDS of PetC gene encoding for the Rieske FeS subunit

Use

Tags

Expression

Plant

Mutation

Codon modified for Golden Gate

Promoter

pZmUbi

Available since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

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DVA_DH

Plasmid

#66049

Purpose

MoClo Destination Vector: (based on pSB1A2 with fixed illegal site) [D:LacZa:H]

Depositor

Douglas Densmore

Article

Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

Type

Empty backbone

Use

Synthetic Biology; Destination vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 21, 2016

Availability

Academic Institutions and Nonprofits only

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DVA_EB

Plasmid

#66050

Purpose

MoClo Destination Vector: (based on pSB1A2 with fixed illegal site) [E:LacZa:B]

Depositor

Douglas Densmore

Article

Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

Type

Empty backbone

Use

Synthetic Biology; Destination vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pJG484

Plasmid

#91194

Purpose

WDV replicon T-DNA for gene targeting in wheat scutella, H840A single nickase (nTaCas9_H840A+gUbi8+donor)

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

nTaCas9_H840A+gUbi8+donor

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

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MSCV T274V KSR1 IRES GFP

Plasmid

#25977

Depositor

Rob Lewis

Article

Razidlo et al J Biol Chem. 2004 Nov 12. 279(46):47808-

Insert

Kinase suppressor of Ras 1 (Ksr1 Mouse)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

changed threonine 274 to valine

Promoter

Available since

Aug. 24, 2010

Availability

Academic Institutions and Nonprofits only
MSCV S392A KSR1 IRES GFP

Plasmid

#25978

Depositor

Rob Lewis

Article

Razidlo et al J Biol Chem. 2004 Nov 12. 279(46):47808-

Insert

Kinase suppressor of Ras 1 (Ksr1 Mouse)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

changed serine 392 to alanine

Promoter

Available since

Aug. 24, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-mETV3

Plasmid

#33374

Depositor

Peter Murray

Article

El Kasmi et al J Immunol. 2007 Dec 1;179(11):7215-9.

Insert

Etv3 (Etv3 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 20, 2011

Availability

Academic Institutions and Nonprofits only
pBad-CA TAG233

Plasmid

#105838

Purpose

Expresses thermostable variant of human carbonic anhydrase II with TAG stop codon at site 233 and C-terminal 6x his tag

Depositor

Ryan Mehl

Article

Mehl et al 2020

Insert

human carbonic anhydrase II thermostable variant (CA2 Human)

Use

Tags

6x his tag

Expression

Bacterial

Mutation

TAG stop codon at site 233

Promoter

pBad/arabinose

Available since

March 14, 2018

Availability

Academic Institutions and Nonprofits only

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pMOD_C3306

Plasmid

#91102

Purpose

Module C, Promoter: FMV 34S, Gene: TALE-VP64 backbone with Esp3I ccdb cassette for repeat cloning, Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

TALE-VP64 backbone with Esp3I ccdb cassette for repeat cloning

Use

TALEN

Tags

Expression

Mutation

Promoter

FMV 34S

Available since

Jan. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-zebrafish lyn(C466A)–GFP

Plasmid

#35957

Depositor

Anna Huttenlocher

Article

Yoo et al Nature. 2011 Nov 20;480(7375):109-12. do

Insert

humanized zebrafish lyn (lyn Zebrafish)

Use

Tags

EGFP

Expression

Mammalian

Mutation

C466A

Promoter

cmv

Available since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

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pVRa27_4265

Plasmid

#49682

Depositor

Christopher Voigt

Article

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

ECF27_4265

Use

Synthetic Biology

Tags

His6-PreScission

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pT7-lacO

Available since

Jan. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pVRb25_up4311

Plasmid

#49717

Depositor

Christopher Voigt

Article

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

sfgfp

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Pecf25_up4311

Available since

Jan. 3, 2014

Availability

Academic Institutions and Nonprofits only

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