-
Plasmid#54611PurposeLocalization: C1 Cloning Vector, Excitation: 558, Emission: 583DepositorTypeEmpty backboneUseTagsDsRed2ExpressionMammalianMutationPromoterAvailable sinceJune 12, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits
-
pDEST17-UbE2B
Plasmid#15781DepositorInsertUbiquitin conjugating enzyme UbE2B (UBE2B Human)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceSept. 28, 2007AvailabilityAcademic Institutions and Nonprofits only -
pRK172-6xHis-0N4R-P301S-tau-HiBiT
Plasmid#187835PurposeExpression of 6xHis-tagged P301S tau of 0N4R isoform with a C-terminal HiBiT tag for bimolecular luminescence with LgBiT.DepositorInsert6XHis-0N4R-P301S-tau-HiBiT
UseTags6xHistidine and HiBiTExpressionBacterialMutationPromoterT7Available sinceSept. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSAM_AraC
Plasmid#91569PurposeMariner transposon mutagenesis & sequencing vector - Transposon contains KanR and Illumina sequencing adapters. Arabinose promoter expresses transposase. Ampicillin resistance on backbone.DepositorInsertAraC/PBAD
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAbaMCi-BsaI_pJMP2776
Plasmid#208890PurposeAcinetobacter baumannii CRISPRi plasmid for cloning new guides (BsaI cloning site in gRNA cassette).DepositorInsertdCas9, sgRNA expression cassette
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pICSL11024 (pICH47732::NOSp-NPTII-OCST)
Plasmid#51144PurposeConfers KAN resistance in plantaDepositorInsertNOSp-NPTII-OCST
UseTagsExpressionMutationPromoterAvailable sinceFeb. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
MP1
Plasmid#69627PurposeUntargetted Bacterial MutagenesisDepositorInsertdnaQ926 umuD' umuC recA730
UseTagsExpressionBacterialMutationPromoterE. coli PBADAvailable sinceSept. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
FUW-tetO-hMYC
Plasmid#20723DepositorInsertc-MYC (MYC Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pAAV-mDlx-NLS-mRuby2 (AAV1)
Viral Prep#99130-AAV1PurposeReady-to-use AAV1 particles produced from pAAV-mDlx-NLS-mRuby2 (#99130). In addition to the viral particles, you will also receive purified pAAV-mDlx-NLS-mRuby2 plasmid DNA. mDlx-driven NLS-mRuby2 expression. These AAV preparations are suitable purity for injection into animals.DepositorPromotermDLX5/6TagsmRuby2Available sinceJan. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
Cultivarium POSSUM Toolkit
Plasmid Kit#1000000234PurposeThe POSSUM Toolkit is a Golden Gate genetic toolkit developed to help researchers identify functional plasmids and establish genetic tractability in non-model microbes.DepositorAvailable sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pX603-AAV-CMV::NLS-dSaCas9(D10A,N580A)-NLS-3xHA-bGHpA
Plasmid#61594PurposeA catalytically inactive SaCas9 (dSaCas9) with the D10A and N580A mutations.DepositorInserthSaCas9
UseAAV and CRISPRTags3xHA and NLSExpressionMammalianMutationD10A, N580APromoterAvailable sinceJuly 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBb(RK2)1k-GFPuv
Plasmid#106394PurposeIPTG-inducible trc promoter expressing gfpuv on RK2 originDepositorInsertGFPuv
UseTagsExpressionBacterialMutationPromotertrcAvailable sinceMarch 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
p28-2iDMD-puro
Plasmid#88895Purpose28°C full-length DMD luc-puro-red plasmid. Co-expresses human DMD, firefly luciferase, puroR and mCherry in mammalian cells. Plasmid carries phiC31 and Bxb1 attB sites, but *cannot* be grown at 37°C.DepositorInsertsUseTagsExpressionMammalianMutationcodon optimizedPromoterAvailable sinceJan. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLminP_Luc2P_RE55
Plasmid#90400PurposeC-REL - NFkB gene reporterDepositorInsertFirefly luciferase
UseLentiviralTagsExpressionMutationPromoterCEBPaAvailable sinceNov. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-RiboL1-jGCaMP8s
Plasmid#192611PurposeAAV transfer plasmid for CAG mediated expression of soma-targeted (RiboL1) jGCaMP8s.DepositorInsertRiboL1-jGCaMP8s
UseAAVTags6xHisExpressionMammalianMutationPromoterAvailable sinceDec. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
Str-Ii_neomycin
Plasmid#65312PurposeCytoplasmic ER hook only (RUSH system)DepositorInsertInvariant chain (p33)
UseTagsExpressionMammalianMutationStreptavidin in its cytoplasmid partPromoterCMVAvailable sinceJune 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
Fuw-dCas9-Dnmt3a_IM
Plasmid#84478PurposeLentiviral construct to express dCas9 fused with an inactive Dnmt3a catalytic domain (E664A, E756A)DepositorInsertdCas9-Dnmt3a_IM
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pORI28
Plasmid#71595PurposeEC1000 containing pORI28. Used as the source of plasmid pORI28. With pTRK669, part of a system for chromosomal integration in gram positive bacteria.DepositorTypeEmpty backboneUseIntegration vector for bacteriaTagsExpressionMutationPromoterAvailable sinceFeb. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
Su9-EGFP
Plasmid#23214DepositorInsertSubunit 9 of the F0-ATPase
UseTagsEGFPExpressionMammalianMutationcontains AA 1-69 of subunit 9PromoterAvailable sinceJan. 28, 2010AvailabilityAcademic Institutions and Nonprofits only -
pERM2-nhpSer
Plasmid#201922PurposeEnables translational incorporation of non-hydrolyzable phosphoserine into proteins at TAG codons in E coli. Do not use for phosphoserine incorporation.DepositorInsertsSepRS(2)
Sep-tRNA v2.0
SerB
EF-Sep
UseTagsNoneExpressionBacterialMutationE412P, E414F, T417K, P495M, I496W, F529S and H67R…PromoterGlnS (constitutive), OXB20 (constitutive), lpp (c…Available sinceJune 7, 2023AvailabilityAcademic Institutions and Nonprofits only